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Gclc (NM_012815) Rat Tagged ORF Clone
CAT＃: RR201392
- TrueORF®
Gclc (Myc-DDK-tagged ORF) - Rat glutamate-cysteine ligase, catalytic subunit (Gclc), (10 ug)
"NM_012815" in other vectors (3)
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CNY 8,270.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Glclc |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR201392 representing NM_012815
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTGCTGTCCCAAGGCTCGCCACTGAGCTGGGAAGAGACCCAGCGCCACGCCGACCACGTGCGGA GACACGGCATCCTCCAGTTCCTGCACATCTACCACGCAGTCAAGGACCGGCACAAGGACGTGCTCAAGTG GGGTGACGAGGTGGAGTACATGTTGGTGTCCTTTGATCATGAAAATAGGAAAGTCCAGTTGTTACTGAAT GGCGGCGATGTTCTTGAAACTCTGCAAGAGAAGGGGGAGAGGACAAACCCCAACCACCCAACCCTCTGGA GACCAGAGTATGGGAGTTACATGATTGAAGGGACACCTGGCCAGCCGTACGGAGGAACGATGTCCGAGTT CAACACAGTGGAGGACAACATGAGGAAACGCCGGAAGGAGGCTACTTCTGTATTAGGAGAACATCAGGCT CTTTGCACGATAACTTCATTTCCCAGGCTAGGCTGCCCTGGATTCACACTGCCAGAGCACAGACCCAACC CAGAGGAAGGAGGTGCATCTAAGTCCCTCTTCTTTCCAGACGAAGCCATAAACAAGCACCCCCGCTTTGG TACTCTAACAAGAAACATCCGGCATCGGAGAGGAGAAAAGGTTGTCATCAATGTGCCAATATTCAAGGAC AAGAACACACCATCTCCGTTTGTAGAAACATTTCCTGAGGATGAGGAGGCATCAAAGGCCTCTAAGCCAG ACCACATCTACATGGATGCCATGGGATTTGGGATGGGCAACTGCTGTCTTCAGGTGACATTCCAAGCCTG CAGTATATCTGAGGCAAGATACCTTTATGACCAGTTGGCCACTATCTGCCCAATTGTTATGGCTTTGAGT GCTGCATCGCCATTTTACCGAGGCTACGTGTCAGACATTGATTGTCGCTGGGGAGTGATTTCTGCATCTG TAGATGATAGAACACGGGAGGAGAGAGGACTGGAGCCCCTGAAGAACAATCGCTTTAAAATCAGTAAGTC TCGGTATGACTCAATAGATAGCTACCTGTCCAAGTGTGGAGAGAAGTACAATGACATCGACCTGACCATC GACACGGAGATCTACGAGCAGCTCTTAGAGGAAGGCATCGATCACCTTCTGGCACAGCACGTTGCTCATC TCTTTATTAGAGACCCACTGACCCTTTTTGAAGAGAAAATTCATCTGGATGATGCCAACGAGTCTGACCA TTTTGAGAATATTCAGTCCACAAACTGGCAGACAATGAGGTTTAAGCCTCCTCCTCCAAACTCAGATATT GGATGGAGAGTAGAGTTCCGACCAATGGAGGTACAGTTGACAGACTTTGAGAACTCTGCCTATGTGGTAT TTGTGGTACTGCTGACCAGGGTGATCCTCTCATACAAACTAGACTTCCTCATTCCACTGTCCAAGGTTGA CGAGAACATGAAAGTGGCACAGGAGCGAGATGCCGTCTTACAGGGGATGTTTTATTTCAGGAAAGACATT TGCAAAGGTGGCAACGCCGTGGTGGATGGGTGTAGCAAGGCCCAGACCAGCTCCGAGCCATCTGCAGAGG AGTACACGCTCATGAGCATAGACACCATCATCAATGGGAAGGAAGGCGTGTTTCCTGGACTCATCCCCAT TCTGAACTCCTACCTTGAAAACATGGAAGTCGACGTGGACACCCGATGCAGTATTCTGAACTACCTGAAG CTAATTAAGAAGAGAGCATCTGGAGAACTAATGACTGTTGCCAGGTGGATGAGAGAGTTTATTGCAAACC ATCCTGACTACAAGCAAGACAGTGTAATAACTGATGAGATCAACTATAGCCTCATTTTGAAATGCAATCA AATTGCAAATGAATTGTGTGAATGTCCAGAGTTACTTGGATCAGGCTTTAGAAAAGCGAAGTACAGTGGA GGTAAAAGCGACCCTTCAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR201392 representing NM_012815
Red=Cloning site Green=Tags(s) MGLLSQGSPLSWEETQRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENRKVQLLLN GGDVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEDNMRKRRKEATSVLGEHQA LCTITSFPRLGCPGFTLPEHRPNPEEGGASKSLFFPDEAINKHPRFGTLTRNIRHRRGEKVVINVPIFKD KNTPSPFVETFPEDEEASKASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALS AASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNRFKISKSRYDSIDSYLSKCGEKYNDIDLTI DTEIYEQLLEEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDI GWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQERDAVLQGMFYFRKDI CKGGNAVVDGCSKAQTSSEPSAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLK LIKKRASGELMTVARWMREFIANHPDYKQDSVITDEINYSLILKCNQIANELCECPELLGSGFRKAKYSG GKSDPSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012815 |
| ORF Size | 1911 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012815.2, NP_036947.1 |
| RefSeq Size | 3079 bp |
| RefSeq ORF | 1914 bp |
| Locus ID | 25283 |
| UniProt ID | P19468 |
| MW | 72.6 kDa |
| Gene Summary | catalyzes the first step in the synthesis of glutathione [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN201392 | Gclc (untagged ORF) - Rat glutamate-cysteine ligase, catalytic subunit (Gclc), (10 ug) |
CNY 8,270.00 |
|
| RR201392L3 | Lenti ORF clone of Gclc (Myc-DDK-tagged ORF) - Rat glutamate-cysteine ligase, catalytic subunit (Gclc), (10 ug) |
CNY 9,980.00 |
|
| RR201392L4 | Lenti ORF clone of Gclc (mGFP-tagged ORF) - Rat glutamate-cysteine ligase, catalytic subunit (Gclc), (10 ug) |
CNY 10,930.00 |
