Egf (NM_012842) Rat Untagged Clone

Name: Egf (NM_012842) Rat Untagged Clone
SKU: RN201131
Price: 11840.00

CAT＃: RN201131

Egf (untagged ORF) - Rat epidermal growth factor (Egf), (10 ug)

Datasheet

SDS

"NM_012842" in other vectors (3)

CNY 11,840.00

货期*

现货

规格

Product images

经常一起买 (2)

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN201131 representing NM_012842 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTTCTCGCTCACCTTCCTGTCGGTGTTTTTAAAGATTACTGTACTCAGTGTCACAGCACAGCAGA CCAGGAACTGTCAGTCAGGTCCTCTCGAGAGAAGCGGGACTACCACGTATGCCGCCGCCGGTCCTCCCAG GTTCCTGATTTTCTTACAAGGAAACAGCATCTTTCGGATTAACACAGATGGAACAAATCACCAGCAATTG GTGGTGGATGCCGGCGTCTCAGTGGTCATGGATTTTCATTACAAGGAAGAGAGACTCTATTGGGTGGATT TAGAAAGACAACTTTTGCAAAGAGTTTTCTTTAATGGGTCAGGACAAGAGACAGTGTGCAAGGTGGATAA GAATGTGTCTGGGCTGGCCATAAACTGGATAGATGGGGAGATTCTCCGGACGGACCGATGGAAGGGAGTC ATCACAGTAACAGATATGAACGGGAACAATTCCCGTGTTCTTCTGAGTTCCTTAAAACGTCCTGCAAATA TATTAGTGGATCCAACAGAGAGGTTGATATTTTGGTCTTCAGTGGTGACTGGCAACCTTCACAGAGCAGA TCTCGGGGGTATGGATGTAAAAACACTGCTGGAGGCACCAGAGAGGATATCAGTGCTGATTCTGGATATC CTGGACAAAAGGCTCTTCTGGGCTCAGGACGGTAGAGAAGGAAGCCACGGTTACATTCACTCCTGTGACT ATAACGGTGGCTCCATCCATCATATCAGACATCAAGCACGGCACGATTTGCTTACTATGGCCATTTTCGG TGACAAGATCTTATACTCAGCACTGAAAGAGAAGGCGATTTGGATAGCCGACAAACACACTGGGAAGAAT GTGGTTCGAGTTAACCTCGATCCAGCCTCTGTGCCGCCAAGAGAACTGAGAGTCGTGCACCTACATGCAC AGCCCGGGACAGAGAACCGTGCTCAGGCCTCTGACTCCGAACGATGCAAACAGAGAAGAGGACAGTGTCT CTACAGTCTCTCTGAGCGAGACCCCAACTCAGACTCGTCGGCATGCGCTGAAGGCTATACGTTAAGCCGA GACCGGAAGTACTGCGAAGATGTCAATGAGTGTGCCTTGCAGAATCACGGCTGTACTCTTGGGTGTGAAA ACATCCCTGGATCCTATTACTGCACATGCCCTACAGGCTTTGTTCTGCTTCCTGATGGGAAACGATGTCA CGAACTTGTTGCCTGTCCAGGCAACAGATCAGAGTGTAGCCATGATTGCATCCTGACATCAGATGGTCCT CTGTGCATCTGTCCAGCAGGTTCAGTGCTCGGAAAAGATGGGAAGACATGCACTGGTTGTTCCTTCTCCG ATAATGGTGGATGCAGCCAGATCTGCCTTCCTCTCAGCCTAGCATCCTGGGAATGTGATTGCTTTCCTGG GTACGACCTACAATTGGACCGAAAGAGCTGTGCAGCTTCCATGGGACCGCAGCCATTTTTACTGTTTGCA AATTCCCAGGACATACGACACATGCATTTTGATGGAACAGACTACAAAACTCTGCTCAGCCGGCAGATGG GAATGGTTTTTGCCTTGGATTATGACCCCGTGGAAAGCAAGATATATTTTGCACAGACAGCCCTGAAGTG GATAGAGAGGGCTAATCTGGATGGCTCCCAGCGAGAAAGACGGATCACGGAAGGAGTAGACACGCCAGAA GGTCTTGCCGTGGACTGGATTGGCCGGAGAATCTACTGGACGGACAGTGGGAAGTCTGTCATTGAAGGGA GTGATTTGAGCGGGAAGCATCATCAAATAATCATCAAAGAGAGCATCTCAAGGCCACGAGGAATAGCTGT GCATCCAAAGGCCAGGAGACTATTCTGGACGGACACGGGGATGTCTCCGCGGATTGAAAGCTCTTCCCTT CAAGGTTCTGACCGGACGCTGATAGCCAGCTCTAATCTACTGGAACCCAGTGGAATCGCGATTGACTACT TAACAGACACTTTGTACTGGTGTGACACCAAGCTGTCTGTGATTGAAATGGCCGATCTAGATGGTTCCAA ACGCCGCAGACTTACCCAGAACGATGTAGGTCACCCATTCTCTCTAGCTGTGTTTGAGGATCACGTGTGG TTCTCGGATTGGGCTATCCCATCGGTAATAAGGGTGAACAAGAGGACTGGTCAAAACAGGGTACGTCTCC GAGGCAGCATGCTGAAGCCCTCGTCACTGGTTGTGGTCCACCCATTGGCAAAACCAGGTGCAGACCCCTG CTTACACAGGAATGGAGGCTGTGAACACATCTGCCAAGAGAGCCTGGGCACGGCTCAGTGTCTGTGTCGG GAAGGATTCGTGAAGGCCCCAGATGGGAAAATGTGTCTCACTCGGAAGGATGATCAGATACTGGCCGGTG ACAATGCTGATCTTAGTAAAGAGGTGGCATCGTTGGACAACTCCCCTAAGGCTTATGTACCAGACGATGA TAGGACAGAGTCCTCCACACTAGTGGCTGAGATCATGGTGTCAGGGCTGAACTATGAAGATGACTGCGGC CCTGGTGGGTGTGGCAGCCATGCCCACTGTATTTCAGAGGGAGAGGCAGCTGTGTGTCAGTGTTTGAAAG GATTTGCTGGCGATGGAAACCTGTGTTCTGATATAGACGAATGTGAGCTGGGTAGCTCAGACTGTCCTCC CACCTCGTCCAGGTGCATCAACACCGAAGGTGGCTATGTCTGCCAATGCTCAGAAGGCTACGAGGGAGAT GGGATCTACTGTCTCGACGTTGATGAGTGCCAGCAGGGGTCGCACGGCTGCAGCGAGAATGCCACCTGCA CCAACACGGAGGGAGGCTACAACTGCACCTGTGCAGGCTGCCCATCAGCACCTGGACTGCCTTGCCCTGA CTCTACCTCACCCTCTCTCCTTGGAAAAGATGGCTGCCACTGGGTCCGAAACAGTAACACAGGATGCCCG CCGTCGTACGATGGGTACTGCCTCAATGGTGGCGTGTGCATGTATGTTGAATCCGTGGACCGCTACGTGT GCAACTGTGTCATTGGCTATATTGGAGAACGATGTCAGCACCGAGACTTACGTTGGTGGAAGCTGCGCCA TGCTGACTACGGGCAGAGGCACGACATCACTGTGGTGTCTGTCTGTGTGGTGGCGCTGGCCCTGCTGCTC CTCTTAGGGATGTGGGGGACTTACTACTACAGGACTCGGAAGCAGCTATCAGAGAGCTCAAAGAAGCCTT CCGAAGAGTCAAGCAGCAACGTGAGCAGTAACGGGCCTGACAGCAGCGGGGCTGGGGTGTCTTCTGGTCC CCAACCTTGGTTTGTGGTCCTAGAGGAACACCAACAGCCCAAGAATGGGCGTCTGCCTGCCGCTGGCACG AACGGCGCAGTAGTAGAGGCTGGCCTGTCTTCCTCCCTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_012842
Insert Size	3402 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_012842.1, NP_036974.1
RefSeq Size	4801 bp
RefSeq ORF	3402 bp
Locus ID	25313
UniProt ID	P07522
Gene Summary	binds the epidermal growth factor receptor; may play a role in MAP kinase mediated signaling pathways [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RR201131	Egf (Myc-DDK-tagged ORF) - Rat epidermal growth factor (Egf), (10 ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 12,624.00
RR201131L3	Lenti ORF clone of Egf (Myc-DDK-tagged ORF) - Rat epidermal growth factor (Egf), (10 ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 19,760.00
RR201131L4	Lenti ORF clone of Egf (mGFP-tagged ORF) - Rat epidermal growth factor (Egf), (10 ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 21,760.00

其它Egf产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies