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CA6 (NM_001270501) Human Tagged ORF Clone
CAT＃: RG232277
- TrueORF®
CA6 (tGFP-tagged) - Homo sapiens carbonic anhydrase VI (CA6), transcript variant 3
ORF Plasmid: DDK
"NM_001270501" in other vectors (2)
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CNY 4,370.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CA-VI; GUSTIN |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG232277 representing NM_001270501
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGCCCTGGTGCTTCTGCTGTCCCTGTTCCTGCTGGGTGGCCAGGCCCAGCATGTGTCTGACTGGA CCTACTCAGTGCAGATCAGCCTGCCCTCCACCATGCGCATGACAGTGGCTGACGGCACTGTATACATAGC CCAGCAGATGCACTTTCACTGGGGAGGTGCGTCCTCGGAGATCAGCGGCTCTGAGCACACCGTGGACGGG ATCAGACATGTGATCGAGATTCACATTGTTCACTACAATTCTAAATACAAGAGCTATGATATAGCCCAAG ATGCGCCGGATGGTTTGGCTGTACTGGCAGCCTTCGTTGAGGTGAAGAATTACCCTGAAAACACTTATTA CAGCAACTTCATTTCTCATCTGGCCAACATCAAGTACCCAGGACAAAGAACAACCCTGACTGGCCTTGAC GTTCAGGACATGCTGCCCAGGAACCTCCAGCACTACTACACCTACCATGGCTCACTCACCACGCCTCCCT GCACTGAGAACGTCCACTGGTTTGTGCTGGCAGATTTTGTCAAGCTCTCCAGGACACAGGTTTGGAAGCT GGAGAATTCCTTACTGGATCACCGCAACAAGACCATCCACAACGATTACCGCAGGACCCAGCCCCTGAAC CACAGAGTGGTGGAATCCAACTTCCCGAATCAGGAATACACTCTAGGCTCTGAATTCCAGTTTTACCTAC ATAAGATTGAGGAAATTCTTGACTACTTAAGAAGAGCATTGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232277 representing NM_001270501
Red=Cloning site Green=Tags(s) MRALVLLLSLFLLGGQAQHVSDWTYSVQISLPSTMRMTVADGTVYIAQQMHFHWGGASSEISGSEHTVDG IRHVIEIHIVHYNSKYKSYDIAQDAPDGLAVLAAFVEVKNYPENTYYSNFISHLANIKYPGQRTTLTGLD VQDMLPRNLQHYYTYHGSLTTPPCTENVHWFVLADFVKLSRTQVWKLENSLLDHRNKTIHNDYRRTQPLN HRVVESNFPNQEYTLGSEFQFYLHKIEEILDYLRRALN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001270501 |
| ORF Size | 744 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001270501.2 |
| RefSeq Size | 1198 bp |
| RefSeq ORF | 747 bp |
| Locus ID | 765 |
| UniProt ID | P23280 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Nitrogen metabolism |
| Gene Summary | The protein encoded by this gene is one of several isozymes of carbonic anhydrase. This protein is found only in salivary glands and saliva and protein may play a role in the reversible hydratation of carbon dioxide though its function in saliva is unknown. [provided by RefSeq, Jul 2008] |
Documents
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