United States
Germany
Japan
United Kingdom
China
DACH2 (NM_001139514) Human Tagged ORF Clone
CAT＃: RG227672
- TrueORF®
DACH2 (tGFP-tagged) - Human dachshund homolog 2 (Drosophila) (DACH2), transcript variant 2
ORF Plasmid: DDK
"NM_001139514" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG227672 representing NM_001139514
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTCTCCGCATCTCCAGTGATCTCTGCAACTTCCAGCGGCGCCGGCGTCCCGGGGGGCTTATTCC GGGCCGAACCCCTGTACTCGACTCCCAGAGAGCCCCCTCGTCTTACTCCTAATATGATCAATAGTTTCGT GGTTAATAACCACAGCAACAGTGCCGGAGGCGGCGGCAGGGGCAACACCAACACCAACGAGTGCCGCATG GTCGACATGCACGGGATGAAGGTGGCTTCGTTCCTGATGGACGGCCAGGAACTGATCTGCCTGCCGCAAG TCTTTGATCTTTTTCTCAAGCACCTGGTGGGAGGCTTGCACACTGTGTACACCAAGCTGAAGAGACTGGA TATATCCCCCGTGGTGTGTACTGTTGAGCAGGTCCGGATCCTCCGCGGGCTGGGGGCCATCCAGCCCGGG GTAAACCGCTGCAAACTCATCACCAGGAAAGACTTCGAAACTTTGTTCACCGATTGCACCAATGCCAGTT CAAGACCCGGCAGGCCCCCTAAGCGTTCTTTGGGAGTGTTGCAGGAAAATGCCCGCCTTCTGACCCATGC AGTCCCAGGCCTCTTATCGCCAGGACTTATCACTCCGACAGGTATAACAGCTGCAGCGATGGCTGAGGCG ATGAAACTTCAGAAGATGAAGCTTATGGCTATGAACACTCTTCAGGGAAATGGAAGCCAAAATGGGACCG AATCAGAGCCTGATGATCTTAATTCTAACACAGGTGGAAGTGAATCCTCCTGGGATAAAGATAAGATGCA GTCTCCATTTGCTGCACCTGGACCCCAACATGGAATTGCTCATGCAGCCCTAGCTGGCCAGCCAGGCATT GGGGGTGCTCCAACCCTCAATCCACTGCAGCAGAACCACCTGCTAACCAATAGACTGGATCTGCCATTTA TGATGATGCCTCATCCCCTACTTCCAGTCAGCTTACCTCCTGCATCAGTTGCCATGGCAATGAATCAGAT GAACCATCTCAATACTATTGCCAACATGGCTGCTGCAGCACAGATTCACAGTCCACTCTCCAGAGCTGGT ACCTCTGTTATAAAGGAGCGGATCCCAGAGAGTCCTTCTCCTGCTCCTTCTCTAGAAGAGAATCATCGTC CTGGGAGCCAGACCTCTTCCCACACCAGCAGCAGTGTGTCCAGCTCTCCCTCTCAGATGGATCATCATTT GGAAAGAATGGAAGAGGTACCAGTTCAAATTCCAATAATGAAGTCACCCTTGGACAAGATACAGCTGACT CCTGGGCAGGCATTGCCCGCTGGATTCCCTGGACCATTCATTTTTGCTGATAGTCTGTCCTCCGTGGAGA CTCTGTTGACCAACATTCAGGGTCTGCTGAAAGTTGCTTTGGATAATGCTCGCATCCAGGAGAAGCAGAT TCAACAAGAAAAGAAGGAGCTGCGACTGGAGCTCTATAGAGAGAGAGAAATTAGAGAAAACCTTGAAAGA CAACTTGCAGTTGAGCTTCAAAGCAGAACTACTATGCAAAAGCGCCTGAAGAAGGAGAAAAAAACCAAGA GAAAATTGCAGGAAGCCTTGGAATTTGAATCAAAGCGCCGGGAGCAAGTGGAGCAGGCACTTAAGCAAGC CACCACTAGTGACAGTGGCCTGAGGATGTTAAAAGATACTGGAATTCCAGATATTGAAATAGAAAACAAT GGGACTCCTCATGATAGTGCTGCTATGCAAGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227672 representing NM_001139514
Red=Cloning site Green=Tags(s) MAVSASPVISATSSGAGVPGGLFRAEPLYSTPREPPRLTPNMINSFVVNNHSNSAGGGGRGNTNTNECRM VDMHGMKVASFLMDGQELICLPQVFDLFLKHLVGGLHTVYTKLKRLDISPVVCTVEQVRILRGLGAIQPG VNRCKLITRKDFETLFTDCTNASSRPGRPPKRSLGVLQENARLLTHAVPGLLSPGLITPTGITAAAMAEA MKLQKMKLMAMNTLQGNGSQNGTESEPDDLNSNTGGSESSWDKDKMQSPFAAPGPQHGIAHAALAGQPGI GGAPTLNPLQQNHLLTNRLDLPFMMMPHPLLPVSLPPASVAMAMNQMNHLNTIANMAAAAQIHSPLSRAG TSVIKERIPESPSPAPSLEENHRPGSQTSSHTSSSVSSSPSQMDHHLERMEEVPVQIPIMKSPLDKIQLT PGQALPAGFPGPFIFADSLSSVETLLTNIQGLLKVALDNARIQEKQIQQEKKELRLELYREREIRENLER QLAVELQSRTTMQKRLKKEKKTKRKLQEALEFESKRREQVEQALKQATTSDSGLRMLKDTGIPDIEIENN GTPHDSAAMQA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001139514 |
| ORF Size | 1713 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001139514.1, NP_001132986.1 |
| RefSeq Size | 2425 bp |
| RefSeq ORF | 1716 bp |
| Locus ID | 117154 |
| UniProt ID | Q96NX9 |
| Protein Families | Transcription Factors |
| Gene Summary | This gene is one of two genes which encode a protein similar to the Drosophila protein dachshund, a transcription factor involved in cell fate determination in the eye, limb and genital disc of the fly. The encoded protein contains two characteristic dachshund domains: an N-terminal domain responsible for DNA binding and a C-terminal domain responsible for protein-protein interactions. This gene is located on the X chromosome and is subject to inactivation by DNA methylation. The encoded protein may be involved in regulation of organogenesis and myogenesis, and may play a role in premature ovarian failure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227672 | DACH2 (Myc-DDK-tagged)-Human dachshund homolog 2 (Drosophila) (DACH2), transcript variant 2 |
CNY 4,256.00 |
|
| RC227672L3 | Lenti-ORF clone of DACH2 (Myc-DDK-tagged)-Human dachshund homolog 2 (Drosophila) (DACH2), transcript variant 2 |
CNY 6,460.00 |
|
| RC227672L4 | Lenti-ORF clone of DACH2 (mGFP-tagged)-Human dachshund homolog 2 (Drosophila) (DACH2), transcript variant 2 |
CNY 6,460.00 |
|
| SC326077 | DACH2 (untagged)-Human dachshund homolog 2 (Drosophila) (DACH2), transcript variant 2 |
CNY 9,220.00 |
