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COQ6 (NM_182480) Human Tagged ORF Clone
CAT＃: RG217898
- TrueORF®
COQ6 (tGFP-tagged) - Human coenzyme Q6 homolog, monooxygenase (S. cerevisiae) (COQ6), nuclear gene encoding mitochondrial protein, transcript variant 2
ORF Plasmid: DDK
"NM_182480" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CGI-10; CGI10; COQ10D6 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217898 representing NM_182480
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATATTCACTTTCATGACAAGAAAATCCTGTTGCTCGAAGCAGGTCCAAAGAAAGTACTGGAGAAATT GTCAGAAACTTACAGCAACAGGGTCAGCTCCATTTCCCCTGGCTCTGCAACGCTTCTCAGTAGTGTGGGA CGCCTGCTCAGAGGCCCTGATAATGTTTGATAAGGATAATTTAGATGACATGGGCTATATCGTGGAGAAT GATGTCATCATGCATGCTCTCACTAAGCAGTTGGAGGCTGTGTCTGACCGAGTGACGGTTCTCTACAGGA GCAAAGCCATTCGCTATACCTGGCCTTGTCCATTTCCTATGGCCGACTCCAGCCCTTGGGTTCATATTAC CCTAGGTGATGGCAGCACCTTCCAGACCAAATTGTTGATAGGTGCAGATGGTCACAACTCCGGAGTACGG CAGGCTGTTGGAATCCAGAATGTGAGCTGGAACTATGACCAGTCTGCTGTTGTGGCTACTCTGCATTTAT CAGAGGCCACAGAAAACAACGTAGCCTGGCAGAGATTTCTTCCCTCTGGGCCTATTGCTCTGCTCCCGCT CTCAGACACCTTGAGTTCCTTGGTTTGGTCCACGTCCCATGAACATGCAGCAGAGCTAGTTAGCATGGAT GAGGAAAAATTTGTGGATGCCGTTAACTCTGCCTTTTGGAGTGATGCTGACCACACGGACTTCATCGACA CAGCTGGTGCCATGCTGCAGTATGCTGTCAGCCTTCTGAAGCCCACTAAGGTCTCGGCTCGCCAGCTGCC CCCAAGCGTAGCCAGGGTGGATGCCAAAAGCCGAGTTCTGTTTCCTCTTGGGTTGGGACATGCTGCTGAG TACGTCAGGCCTCGGGTGGCGCTCATTGGGGATGCAGCCCACAGAGTCCATCCGCTTGCAGGACAGGGTG TCAACATGGGCTTTGGGGATATCTCCAGCTTGGCCCATCACCTCAGTACGGCAGCCTTCAATGGGAAGGA CTTAGGTTCCGTGAGCCACCTCACAGGTTATGAAACAGAAAGACAGCGTCACAACACTGCTCTTCTGGCT GCTACAGACTTACTAAAAAGGCTCTATTCTACCAGTGCCTCCCCGCTTGTGTTGCTCAGGACGTGGGGCT TGCAGGCCACAAATGCAGTGTCTCCACTCAAAGAACAGATTATGGCCTTTGCAAGCAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217898 representing NM_182480
Red=Cloning site Green=Tags(s) MIFTFMTRKSCCSKQVQRKYWRNCQKLTATGSAPFPLALQRFSVVWDACSEALIMFDKDNLDDMGYIVEN DVIMHALTKQLEAVSDRVTVLYRSKAIRYTWPCPFPMADSSPWVHITLGDGSTFQTKLLIGADGHNSGVR QAVGIQNVSWNYDQSAVVATLHLSEATENNVAWQRFLPSGPIALLPLSDTLSSLVWSTSHEHAAELVSMD EEKFVDAVNSAFWSDADHTDFIDTAGAMLQYAVSLLKPTKVSARQLPPSVARVDAKSRVLFPLGLGHAAE YVRPRVALIGDAAHRVHPLAGQGVNMGFGDISSLAHHLSTAAFNGKDLGSVSHLTGYETERQRHNTALLA ATDLLKRLYSTSASPLVLLRTWGLQATNAVSPLKEQIMAFASK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_182480 |
| ORF Size | 1179 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_182480.1, NP_872286.1 |
| RefSeq Size | 1553 bp |
| RefSeq ORF | 1332 bp |
| Locus ID | 51004 |
| UniProt ID | Q9Y2Z9 |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Ubiquinone and other terpenoid-quinone biosynthesis |
| Gene Summary | The protein encoded by this gene belongs to the ubiH/COQ6 family. It is an evolutionarily conserved monooxygenase required for the biosynthesis of coenzyme Q10 (or ubiquinone), which is an essential component of the mitochondrial electron transport chain, and one of the most potent lipophilic antioxidants implicated in the protection of cell damage by reactive oxygen species. Knockdown of this gene in mouse and zebrafish results in decreased growth due to increased apoptosis. Mutations in this gene are associated with autosomal recessive coenzyme Q10 deficiency-6 (COQ10D6), which manifests as nephrotic syndrome with sensorineural deafness. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217898 | COQ6 (Myc-DDK-tagged)-Human coenzyme Q6 homolog, monooxygenase (S. cerevisiae) (COQ6), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 3,656.00 |
|
| RC217898L3 | Lenti ORF clone of Human coenzyme Q6 homolog, monooxygenase (S. cerevisiae) (COQ6), nuclear gene encoding mitochondrial protein, transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217898L4 | Lenti ORF clone of Human coenzyme Q6 homolog, monooxygenase (S. cerevisiae) (COQ6), nuclear gene encoding mitochondrial protein, transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| SC307400 | COQ6 (untagged)-Human coenzyme Q6 homolog, monooxygenase (S. cerevisiae) (COQ6), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 6,370.00 |
