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ECE2 (EEF1AKMT4) (NM_001100120) Human Tagged ORF Clone
CAT＃: RG214966
- TrueORF®
ECE2 (tGFP-tagged) - Human endothelin converting enzyme 2 (ECE2), transcript variant 4
ORF Plasmid: DDK
"NM_001100120" in other vectors (4)
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CNY 8,930.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | EEF1AKMT4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214966 representing NM_001100120
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGTCGCGCTGCAGGAGCTGGGAGCTGGCAGCAACATGGTGGAGTACAAACGGGCCACGCTTCGGG ATGAAGACGCACCCGAGACCCCCGTAGAGGGCGGGGCCTCCCCGGACGCCATGGAGGTGGGCAAGGGGGC TTCCCCTTTCTCACCAGGCCCCAGCCCTGGCATGACGCCTGGCACACCCAGGAGCTCTGGGCTGTTCTGG AGGGTCACCTGCCCCCACCTCCGCTCCATCTCTGGCCTCTGCTCTAGGACTATGGTGGGATTCCAGAAGG GGACAAGACAGCTGTTAGGCTCACGCACGCAGCTGGAGCTGGTCTTAGCAGGTGCCTCTCTACTGCTGGC TGCACTGCTTCTGGGCTGCCTTGTGGCCCTAGGGGTCCAGTACCACAGAGACCCATCCCACAGCACCTGC CTTACAGAGGCCTGCATTCGAGTGGCTGGAAAAATCCTGGAGTCCCTGGACCGAGGGGTGAGCCCCTGTG AGGACTTTTACCAGTTCTCCTGTGGGGGCTGGATTCGGAGGAACCCCCTGCCCGATGGGCGTTCTCGCTG GAACACCTTCAACAGCCTCTGGGACCAAAACCAGGCCATACTGAAGCACCTGCTTGAAAACACCACCTTC AACTCCAGCAGTGAAGCTGAGCAGAAGACACAGCGCTTCTACCTATCTTGCCTACAGGTGGAGCGCATTG AGGAGCTGGGAGCCCAGCCACTGAGAGACCTCATTGAGAAGATTGGTGGTTGGAACATTACGGGGCCCTG GGACCAGGACAACTTTATGGAGGTGTTGAAGGCAGTAGCAGGGACCTACAGGGCCACCCCATTCTTCACC GTCTACATCAGTGCCGACTCTAAGAGTTCCAACAGCAATGTTATCCAGGTGGACCAGTCTGGGCTCTTTC TGCCCTCTCGGGATTACTACTTAAACAGAACTGCCAATGAGAAAGTGCTCACTGCCTATCTGGATTACAT GGAGGAACTGGGGATGCTGCTGGGTGGGCGGCCCACCTCCACGAGGGAGCAGATGCAGCAGGTGCTGGAG TTGGAGATACAGCTGGCCAACATCACAGTGCCCCAGGACCAGCGGCGCGACGAGGAGAAGATCTACCACA AGATGAGCATTTCGGAGCTGCAGGCTCTGGCGCCCTCCATGGACTGGCTTGAGTTCCTGTCTTTCTTGCT GTCACCATTGGAGTTGAGTGACTCTGAGCCTGTGGTGGTGTATGGGATGGATTATTTGCAGCAGGTGTCA GAGCTCATCAACCGCACGGAACCAAGCATCCTGAACAATTACCTGATCTGGAACCTGGTGCAAAAGACAA CCTCAAGCCTGGACCGACGCTTTGAGTCTGCACAAGAGAAGCTGCTGGAGACCCTCTATGGCACTAAGAA GTCCTGTGTGCCGAGGTGGCAGACCTGCATCTCCAACACGGATGACGCCCTTGGCTTTGCTTTGGGGTCC CTCTTCGTGAAGGCCACGTTTGACCGGCAAAGCAAAGAAATTGCAGAGGGGATGATCAGCGAAATCCGGA CCGCATTTGAGGAGGCCCTGGGACAGCTGGTTTGGATGGATGAGAAGACCCGCCAGGCAGCCAAGGAGAA AGCAGATGCCATCTATGATATGATTGGTTTCCCAGACTTTATCCTGGAGCCCAAAGAGCTGGATGATGTT TATGACGGGTACGAAATTTCTGAAGATTCTTTCTTCCAAAACATGTTGAATTTGTACAACTTCTCTGCCA AGGTTATGGCTGACCAGCTCCGCAAGCCTCCCAGCCGAGACCAGTGGAGCATGACCCCCCAGACAGTGAA TGCCTACTACCTTCCAACTAAGAATGAGATCGTCTTCCCCGCTGGCATCCTGCAGGCCCCCTTCTATGCC CGCAACCACCCCAAGGCCCTGAACTTCGGTGGCATCGGTGTGGTCATGGGCCATGAGTTGACGCATGCCT TTGATGACCAAGGGCGCGAGTATGACAAAGAAGGGAACCTGCGGCCCTGGTGGCAGAATGAGTCCCTGGC AGCCTTCCGGAACCACACGGCCTGCATGGAGGAACAGTACAATCAATACCAGGTCAATGGGGAGAGGCTC AACGGCCGCCAGACGCTGGGGGAGAACATTGCTGACAACGGGGGGCTGAAGGCTGCCTACAATGCTTACA AAGCATGGCTGAGAAAGCATGGGGAGGAGCAGCAACTGCCAGCCGTGGGGCTCACCAACCACCAGCTCTT CTTCGTGGGATTTGCCCAGGTGTGGTGCTCGGTCCGCACACCAGAGAGCTCTCACGAGGGGCTGGTGACC GACCCCCACAGCCCTGCCCGCTTCCGCGTGCTGGGCACTCTCTCCAACTCCCGTGACTTCCTGCGGCACT TCGGCTGCCCTGTCGGCTCCCCCATGAACCCAGGGCAGCTGTGTGAGGTGTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214966 representing NM_001100120
Red=Cloning site Green=Tags(s) MNVALQELGAGSNMVEYKRATLRDEDAPETPVEGGASPDAMEVGKGASPFSPGPSPGMTPGTPRSSGLFW RVTCPHLRSISGLCSRTMVGFQKGTRQLLGSRTQLELVLAGASLLLAALLLGCLVALGVQYHRDPSHSTC LTEACIRVAGKILESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENTTF NSSSEAEQKTQRFYLSCLQVERIEELGAQPLRDLIEKIGGWNITGPWDQDNFMEVLKAVAGTYRATPFFT VYISADSKSSNSNVIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGMLLGGRPTSTREQMQQVLE LEIQLANITVPQDQRRDEEKIYHKMSISELQALAPSMDWLEFLSFLLSPLELSDSEPVVVYGMDYLQQVS ELINRTEPSILNNYLIWNLVQKTTSSLDRRFESAQEKLLETLYGTKKSCVPRWQTCISNTDDALGFALGS LFVKATFDRQSKEIAEGMISEIRTAFEEALGQLVWMDEKTRQAAKEKADAIYDMIGFPDFILEPKELDDV YDGYEISEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYA RNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLAAFRNHTACMEEQYNQYQVNGERL NGRQTLGENIADNGGLKAAYNAYKAWLRKHGEEQQLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVT DPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001100120 |
| ORF Size | 2433 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001100120.2 |
| RefSeq Size | 3387 bp |
| RefSeq ORF | 2436 bp |
| Locus ID | 9718 |
| UniProt ID | P0DPD6 |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| Gene Summary | Converts big endothelin-1 to endothelin-1. May also have methyltransferase activity (By similarity). May play a role in amyloid-beta processing (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214966 | ECE2 (Myc-DDK-tagged)-Human endothelin converting enzyme 2 (ECE2), transcript variant 4 |
CNY 8,080.00 |
|
| RC214966L3 | Lenti-ORF clone of ECE2 (Myc-DDK-tagged)-Human endothelin converting enzyme 2 (ECE2), transcript variant 4 |
CNY 9,980.00 |
|
| RC214966L4 | Lenti-ORF clone of ECE2 (mGFP-tagged)-Human endothelin converting enzyme 2 (ECE2), transcript variant 4 |
CNY 9,980.00 |
|
| SC316837 | ECE2 (untagged)-Human endothelin converting enzyme 2 (ECE2), transcript variant 4 |
CNY 15,580.00 |
