SOCS3 (NM_003955) Human Tagged ORF Clone
CAT#: RG209305
- TrueORF®
SOCS3 (tGFP-tagged) - Human suppressor of cytokine signaling 3 (SOCS3)
ORF Plasmid: DDK
"NM_003955" in other vectors (6)
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CNY 5,200.00
Cited in 2 publications. |
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ATOD4; CIS3; Cish3; SOCS-3; SSI-3; SSI3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG209305 representing NM_003955
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCACCCACAGCAAGTTTCCCGCCGCCGGGATGAGCCGCCCCCTGGACACCAGCCTGCGCCTCAAGA CCTTCAGCTCCAAGAGCGAGTACCAGCTGGTGGTGAACGCAGTGCGCAAGCTGCAGGAGAGCGGCTTCTA CTGGAGCGCAGTGACCGGCGGCGAGGCGAACCTGCTGCTCAGTGCCGAGCCCGCCGGCACCTTTCTGATC CGCGACAGCTCGGACCAGCGCCACTTCTTCACGCTCAGCGTCAAGACCCAGTCTGGGACCAAGAACCTGC GCATCCAGTGTGAGGGGGGCAGCTTCTCTCTGCAGAGCGATCCCCGGAGCACGCAGCCCGTGCCCCGCTT CGACTGCGTGCTCAAGCTGGTGCACCACTACATGCCGCCCCCTGGAGCCCCCTCCTTCCCCTCGCCACCT ACTGAACCCTCCTCCGAGGTGCCCGAGCAGCCGTCTGCCCAGCCACTCCCTGGGAGTCCCCCCAGAAGAG CCTATTACATCTACTCCGGGGGCGAGAAGATCCCCCTGGTGTTGAGCCGGCCCCTCTCCTCCAACGTGGC CACTCTTCAGCATCTCTGTCGGAAGACCGTCAACGGCCACCTGGACTCCTATGAGAAAGTCACCCAGCTG CCGGGGCCCATTCGGGAGTTCCTGGACCAGTACGATGCCCCGCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209305 representing NM_003955
Red=Cloning site Green=Tags(s) MVTHSKFPAAGMSRPLDTSLRLKTFSSKSEYQLVVNAVRKLQESGFYWSAVTGGEANLLLSAEPAGTFLI RDSSDQRHFFTLSVKTQSGTKNLRIQCEGGSFSLQSDPRSTQPVPRFDCVLKLVHHYMPPPGAPSFPSPP TEPSSEVPEQPSAQPLPGSPPRRAYYIYSGGEKIPLVLSRPLSSNVATLQHLCRKTVNGHLDSYEKVTQL PGPIREFLDQYDAPL TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_003955 |
ORF Size | 675 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003955.5 |
RefSeq Size | 2746 bp |
RefSeq ORF | 678 bp |
Locus ID | 9021 |
UniProt ID | O14543 |
Domains | SH2, SOCS |
Protein Families | Druggable Genome |
Protein Pathways | Adipocytokine signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, Type II diabetes mellitus, Ubiquitin mediated proteolysis |
Gene Summary | This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. [provided by RefSeq, Jul 2008] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Human Th9 differentiation is dependent upon STAT3 to restrain STAT1-mediated inhibition
,Zhang, Y;Siegel, AM;Sun, G;Dimaggio, T;Freeman, AF;Milner, JD;,
J. Allergy Clin. Immunol.
,PubMed ID 30030006
[SOCS3]
|
PD-L1 up-regulation restrains Th17 cell differentiation in STAT3 loss- and STAT1 gain-of-function patients
,Zhang, Y;Ma, CA;Lawrence, MG;Break, TJ;O'Connell, MP;Lyons, JJ;López, DB;Barber, JS;Zhao, Y;Barber, DL;Freeman, AF;Holland, SM;Lionakis, MS;Milner, JD;,
J. Exp. Med.
,PubMed ID 28710273
[SOCS3]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209305 | SOCS3 (Myc-DDK-tagged)-Human suppressor of cytokine signaling 3 (SOCS3) |
CNY 3,600.00 |
|
RC209305L1 | Lenti ORF clone of Human suppressor of cytokine signaling 3 (SOCS3), Myc-DDK-tagged |
CNY 6,000.00 |
|
RC209305L2 | Lenti ORF clone of Human suppressor of cytokine signaling 3 (SOCS3), mGFP tagged |
CNY 6,000.00 |
|
RC209305L3 | Lenti ORF clone of Human suppressor of cytokine signaling 3 (SOCS3), Myc-DDK-tagged |
CNY 6,000.00 |
|
RC209305L4 | Lenti ORF clone of Human suppressor of cytokine signaling 3 (SOCS3), mGFP tagged |
CNY 6,000.00 |
|
SC117660 | SOCS3 (untagged)-Human suppressor of cytokine signaling 3 (SOCS3) |
CNY 3,600.00 |