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PLC delta 3 (PLCD3) (NM_133373) Human Tagged ORF Clone
CAT＃: RG209120
- TrueORF®
PLCD3 (tGFP-tagged) - Human phospholipase C, delta 3 (PLCD3)
ORF Plasmid: DDK
"NM_133373" in other vectors (6)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PLC-delta-3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG209120 representing NM_133373
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTGCGGCCGCTGGAGGCGTTGCCGCCGCCCGCCCGAGGAGCCCCCGGTGGCCGCCCAGGTCGCAG CCCAAGTCGCGGCGCCGGTCGCTCTCCCGTCCCCGCCGACTCCCTCCGATGGCGGCACCAAGAGGCCCGG GCTGCGGGCGCTGAAGAAGATGGGCCTGACGGAGGACGAGGACGTGCGCGCCATGCTGCGGGGCTCCCGG CTCCGCAAGATCCGCTCGCGCACGTGGCACAAGGAGCGGCTGTACCGGCTGCAGGAGGACGGCCTGAGCG TGTGGTTCCAGCGGCGCATCCCGCGTGCGCCATCGCAGCACATCTTCTTCGTGCAGCACATCGAGGCGGT CCGCGAGGGCCACCAGTCCGAGGGCCTGCGGCGCTTCGGGGGTGCCTTCGCGCCAGCGCGCTGCCTCACC ATCGCCTTCAAGGGCCGCCGCAAGAACCTGGACCTGGCGGCGCCCACGGCTGAGGAAGCGCAGCGCTGGG TGCGCGGTCTGACCAAGCTCCGCGCGCGCCTGGACGCCATGAGCCAGCGCGAGCGGCTAGACCACTGGAT CCACTCCTATCTGCACCGGGCTGACTCCAACCAGGACAGCAAGATGAGCTTCAAGGAGATCAAGAGCCTG CTGAGAATGGTCAACGTGGACATGAACGACATGTACGCCTACCTCCTCTTCAAGGAGTGTGACCACTCCA ACAACGACCGTCTAGAGGGGGCTGAGATCGAGGAGTTCCTGCGGCGGCTGCTGAAGCGGCCGGAGCTGGA GGAGATCTTCCATCAGTACTCGGGCGAGGACCGCGTGCTGAGTGCCCCTGAGCTGCTGGAGTTCCTGGAG GACCAGGGCGAGGAGGGCGCCACACTGGCCCGCGCCCAGCAGCTCATTCAGACCTATGAGCTCAACGAGA CAGCCAAGCAGCATGAGCTGATGACACTGGATGGCTTCATGATGTACCTGTTGTCGCCGGAGGGGGCTGC CTTGGACAACACCCACACGTGTGTGTTCCAGGACATGAACCAGCCCCTTGCCCACTACTTCATCTCTTCC TCCCACAACACCTATCTGACTGACTCCCAGATCGGGGGGCCCAGCAGCACCGAGGCCTATGTTAGGGCCT TTGCCCAGGGATGCCGCTGCGTGGAGCTGGACTGCTGGGAGGGGCCAGGAGGGGAGCCCGTCATCTATCA TGGCCATACCCTCACCTCCAAGATTCTCTTCCGGGACGTGGTCCAAGCCGTGCGCGACCATGCCTTCACG CTGTCCCCTTACCCTGTCATCCTATCCCTGGAGAACCACTGCGGGCTGGAGCAGCAGGCTGCCATGGCCC GCCACCTCTGCACCATCCTGGGGGACATGCTGGTGACACAGGCGCTGGACTCCCCAAATCCCGAGGAGCT GCCATCCCCAGAGCAGCTGAAGGGCCGGGTCCTGGTGAAGGGAAAGAAGCTGCCCGCTGCTCGGAGCGAG GATGGCCGGGCTCTGTCGGATCGGGAGGAGGAGGAGGAGGATGACGAGGAGGAAGAAGAGGAGGTGGAGG CTGCAGCGCAGAGGCGGCTGGCCAAGCAGATCTCCCCGGAGCTGTCGGCCCTGGCTGTGTACTGCCACGC CACCCGCCTGCGGACCCTGCACCCTGCCCCCAACGCCCCACAACCCTGCCAGGTCAGCTCCCTCAGCGAG CGCAAAGCCAAGAAACTCATTCGGGAGGCAGGGAACAGCTTTGTCAGGCACAATGCCCGCCAGCTGACCC GCGTGTACCCGCTGGGGCTGCGGATGAACTCAGCCAACTACAGTCCCCAGGAGATGTGGAACTCGGGCTG TCAGCTGGTGGCCTTGAACTTCCAGACGCCAGGCTACGAGATGGACCTCAATGCCGGGCGCTTCCTAGTC AATGGGCAGTGTGGCTACGTCCTAAAACCTGCCTGCCTGCGGCAACCTGACTCGACCTTTGACCCCGAGT ACCCAGGACCTCCCAGAACCACTCTCAGCATCCAGGTGCTGACTGCACAGCAGCTGCCCAAGCTGAATGC CGAGAAGCCACACTCCATTGTGGACCCCCTGGTGCGCATTGAGATCCATGGGGTGCCCGCAGACTGTGCC CGGCAGGAGACTGACTACGTGCTCAACAATGGCTTCAACCCCCGCTGGGGGCAGACCCTGCAGTTCCAGC TGCGGGCTCCGGAGCTGGCACTGGTCCGGTTTGTGGTGGAAGATTATGACGCCACCTCCCCCAATGACTT TGTGGGCCAGTTTACACTGCCTCTTAGCAGCCTAAAGCAAGGGTACCGCCACATACACCTGCTTTCCAAG GACGGGGCCTCACTGTCACCAGCCACGCTCTTCATCCAAATCCGCATCCAGCGCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209120 representing NM_133373
Red=Cloning site Green=Tags(s) MLCGRWRRCRRPPEEPPVAAQVAAQVAAPVALPSPPTPSDGGTKRPGLRALKKMGLTEDEDVRAMLRGSR LRKIRSRTWHKERLYRLQEDGLSVWFQRRIPRAPSQHIFFVQHIEAVREGHQSEGLRRFGGAFAPARCLT IAFKGRRKNLDLAAPTAEEAQRWVRGLTKLRARLDAMSQRERLDHWIHSYLHRADSNQDSKMSFKEIKSL LRMVNVDMNDMYAYLLFKECDHSNNDRLEGAEIEEFLRRLLKRPELEEIFHQYSGEDRVLSAPELLEFLE DQGEEGATLARAQQLIQTYELNETAKQHELMTLDGFMMYLLSPEGAALDNTHTCVFQDMNQPLAHYFISS SHNTYLTDSQIGGPSSTEAYVRAFAQGCRCVELDCWEGPGGEPVIYHGHTLTSKILFRDVVQAVRDHAFT LSPYPVILSLENHCGLEQQAAMARHLCTILGDMLVTQALDSPNPEELPSPEQLKGRVLVKGKKLPAARSE DGRALSDREEEEEDDEEEEEEVEAAAQRRLAKQISPELSALAVYCHATRLRTLHPAPNAPQPCQVSSLSE RKAKKLIREAGNSFVRHNARQLTRVYPLGLRMNSANYSPQEMWNSGCQLVALNFQTPGYEMDLNAGRFLV NGQCGYVLKPACLRQPDSTFDPEYPGPPRTTLSIQVLTAQQLPKLNAEKPHSIVDPLVRIEIHGVPADCA RQETDYVLNNGFNPRWGQTLQFQLRAPELALVRFVVEDYDATSPNDFVGQFTLPLSSLKQGYRHIHLLSK DGASLSPATLFIQIRIQRS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133373 |
| ORF Size | 2367 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133373.2, NP_588614.1 |
| RefSeq Size | 3477 bp |
| RefSeq ORF | 2370 bp |
| Locus ID | 113026 |
| UniProt ID | Q8N3E9 |
| Domains | C2, PI-PLC-X, PI-PLC-Y, PH |
| Protein Families | Druggable Genome |
| Protein Pathways | Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| Gene Summary | This gene encodes a member of the phospholipase C family, which catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate the second messengers diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Diacylglycerol and IP3 mediate a variety of cellular responses to extracellular stimuli by inducing protein kinase C and increasing cytosolic Ca(2+) concentrations. This enzyme localizes to the plasma membrane and requires calcium for activation. Its activity is inhibited by spermine, sphingosine, and several phospholipids. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209120 | PLCD3 (Myc-DDK-tagged)-Human phospholipase C, delta 3 (PLCD3) |
CNY 8,664.00 |
|
| RC209120L1 | Lenti-ORF clone of PLCD3 (Myc-DDK-tagged)-Human phospholipase C, delta 3 (PLCD3) |
CNY 11,064.00 |
|
| RC209120L2 | Lenti-ORF clone of PLCD3 (mGFP-tagged)-Human phospholipase C, delta 3 (PLCD3) |
CNY 5,890.00 |
|
| RC209120L3 | Lenti-ORF clone of PLCD3 (Myc-DDK-tagged)-Human phospholipase C, delta 3 (PLCD3) |
CNY 5,890.00 |
|
| RC209120L4 | Lenti-ORF clone of PLCD3 (mGFP-tagged)-Human phospholipase C, delta 3 (PLCD3) |
CNY 5,890.00 |
|
| SC120539 | PLCD3 (untagged)-Human phospholipase C, delta 3 (PLCD3) |
CNY 2,950.00 |
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