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JRK (NM_003724) Human Tagged ORF Clone
CAT＃: RG208137
- TrueORF®
JRK (tGFP-tagged) - Human jerky homolog (mouse) (JRK), transcript variant 1
ORF Plasmid: DDK
"NM_003724" in other vectors (6)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | jerky; JH8 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208137 representing NM_003724
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCCAAGCCGGCTGCCGGGAAGAGCAGAGGGGAGAAGCGGAAGAGGGTGGTGCTGACACTGAAGG AGAAGATTGACATCTGCACGCGCCTGGAGAAGGGCGAGAGCCGGAAGGCACTGATGCAGGAGTACAATGT GGGCATGTCCACCCTCTACGACATCAGGGCCCACAAGGCGCAGCTGCTCCGGTTCTTCGCCAGCTCCGAC TCCAACAAGGCGCTGGAGCAGCGGCGCACGCTGCACACGCCCAAGCTGGAGCACCTGGACCGCGTCCTGT ACGAGTGGTTCCTGGGGAAGCGCTCCGAGGGCGTCCCCGTGTCAGGCCCCATGCTCATCGAGAAGGCCAA GGACTTCTACGAGCAGATGCAGCTCACTGAGCCCTGCGTGTTCTCCGGAGGGTGGCTTTGGCGCTTTAAG GCCAGACACGGCATTAAAAAGCTAGATGCATCCAGTGAAAAGCAGTCAGCCGACCACCAGGCCGCGGAGC AGTTCTGTGCGTTTTTCAGGAGCTTGGCTGCTGAGCACGGGCTGTCCGCCGAGCAGGTTTACAACGCTGA TGAGACCGGCCTTTTCTGGCGGTGCCTGCCAAATCCCACTCCGGAAGGCGGGGCTGTGCCTGGCCCCAAG CAGGGCAAGGACCGGCTGACCGTGCTGATGTGTGCCAACGCCACGGGCTCCCACAGGCTCAAGCCCTTGG CCATCGGGAAGTGCAGCGGTCCCAGGGCTTTCAAAGGCATCCAGCACCTGCCCGTCGCCTATAAGGCCCA GGGGAACGCCTGGGTGGACAAGGAGATTTTTTCCGATTGGTTCCATCATATCTTTGTGCCCTCGGTGAGA GAGCACTTCAGAACCATAGGTTTGCCGGAAGACAGCAAAGCCGTTCTCTTGCTGGACAGCTCCCGGGCTC ACCCGCAGGAGGCCGAGCTGGTGTCCAGTAACGTTTTCACCATCTTCCTGCCTGCCAGCGTGGCCTCATT GGTGCAGCCCATGGAGCAGGGCATTCGGAGAGATTTCATGAGGAACTTCATTAACCCTCCGGTCCCCCTG CAGGGCCCCCACGCCCGCTACAACATGAACGATGCCATATTCAGCGTGGCCTGTGCCTGGAACGCAGTCC CTAGCCACGTCTTCAGGCGGGCCTGGAGGAAGCTGTGGCCGTCGGTTGCGTTTGCCGAAGGCTCCTCCTC TGAGGAGGAGTTGGAGGCAGAGTGCTTCCCAGTGAAGCCCCACAACAAGTCCTTTGCACACATCCTGGAG CTTGTGAAGGAAGGCTCCTCCTGCCCGGGCCAGCTTCGCCAGCGCCAGGCCGCCAGCTGGGGGGTAGCGG GAAGGGAGGCAGAAGGGGGACGGCCCCCTGCTGCCACGTCGCCAGCAGAGGTTGTGTGGAGTTCAGAAAA GACTCCGAAAGCTGACCAGGACGGCAGAGGAGATCCTGGTGAGGGCGAGGAGGTGGCCTGGGAGCAGGCG GCCGTGGCCTTTGACGCAGTCCTGCGCTTTGCGGAGCGGCAGCCATGCTTCAGTGCGCAGGAAGTGGGGC AGCTGCGGGCGCTGCGTGCCGTGTTCCGGAGCCAGCAGCAGGTGAGGAGGCGGCGTGGTGCCCTCGGGGC TGTGGTCAAGGTTGAAGCCCTCCAGGAGGGCCCTGGTGGCTGTGGGGCCACAGCTCAGTCTCCCTTGCCC TGCTCATCCACAGCAGGTGACAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208137 representing NM_003724
Red=Cloning site Green=Tags(s) MASKPAAGKSRGEKRKRVVLTLKEKIDICTRLEKGESRKALMQEYNVGMSTLYDIRAHKAQLLRFFASSD SNKALEQRRTLHTPKLEHLDRVLYEWFLGKRSEGVPVSGPMLIEKAKDFYEQMQLTEPCVFSGGWLWRFK ARHGIKKLDASSEKQSADHQAAEQFCAFFRSLAAEHGLSAEQVYNADETGLFWRCLPNPTPEGGAVPGPK QGKDRLTVLMCANATGSHRLKPLAIGKCSGPRAFKGIQHLPVAYKAQGNAWVDKEIFSDWFHHIFVPSVR EHFRTIGLPEDSKAVLLLDSSRAHPQEAELVSSNVFTIFLPASVASLVQPMEQGIRRDFMRNFINPPVPL QGPHARYNMNDAIFSVACAWNAVPSHVFRRAWRKLWPSVAFAEGSSSEEELEAECFPVKPHNKSFAHILE LVKEGSSCPGQLRQRQAASWGVAGREAEGGRPPAATSPAEVVWSSEKTPKADQDGRGDPGEGEEVAWEQA AVAFDAVLRFAERQPCFSAQEVGQLRALRAVFRSQQQVRRRRGALGAVVKVEALQEGPGGCGATAQSPLP CSSTAGDN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003724 |
| ORF Size | 1704 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003724.4 |
| RefSeq Size | 9116 bp |
| RefSeq ORF | 1707 bp |
| Locus ID | 8629 |
| UniProt ID | O75564 |
| Domains | DDE, CENPB, CENP-B_N |
| Protein Families | Transcription Factors |
| Gene Summary | This gene encodes a conserved protein that is similar to DNA-binding proteins, such as major centromere autoantigen B (CENPB). Inactivation of the related gene in mice resulted in epileptic seizures. Childhood Absence Epilepsy (CAE) has been mapped to the same chromosomal location (8q24.3) as this gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208137 | JRK (Myc-DDK-tagged)-Human jerky homolog (mouse) (JRK), transcript variant 1 |
CNY 6,344.00 |
|
| RC208137L1 | Lenti-ORF clone of JRK (Myc-DDK-tagged)-Human jerky homolog (mouse) (JRK), transcript variant 1 |
CNY 8,744.00 |
|
| RC208137L2 | Lenti-ORF clone of JRK (mGFP-tagged)-Human jerky homolog (mouse) (JRK), transcript variant 1 |
CNY 6,460.00 |
|
| RC208137L3 | Lenti-ORF clone of JRK (Myc-DDK-tagged)-Human jerky homolog (mouse) (JRK), transcript variant 1 |
CNY 6,460.00 |
|
| RC208137L4 | Lenti-ORF clone of JRK (mGFP-tagged)-Human jerky homolog (mouse) (JRK), transcript variant 1 |
CNY 6,460.00 |
|
| SC100271 | JRK (untagged)-Human jerky homolog (mouse) (JRK), transcript variant 1 |
CNY 6,352.00 |
