MAG (NM_001199216) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GMA; S-MAG; SIGLEC-4A; SIGLEC4A; SPG75
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234527 representing NM_001199216 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTCGTCCATCTCGGCCTTCGAAGGCACGTGCGTCTCCATCCCCTGCCGCTTTGACTTCCCGGATG AGCTGCGGCCCGCTGTGGTGCATGGTGTCTGGTACTTCAATAGCCCCTACCCCAAGAACTACCCCCCGGT GGTCTTCAAGTCGCGCACCCAAGTAGTCCACGAGAGCTTCCAGGGCCGCAGCCGCCTCCTGGGGGACCTG GGCCTGCGAAACTGCACCCTCCTGCTCAGCAACGTCAGCCCCGAGCTGGGCGGGAAGTACTACTTCCGTG GGGACCTGGGCGGCTACAACCAGTACACCTTCTCAGAGCACAGCGTCCTGGATATCGTCAACACCCCCAA CATCGTGGTGCCCCCAGAGGTGGTGGCAGGCACGGAGGTGGAGGTCAGCTGCATGGTGCCGGACAACTGC CCAGAGCTGCGCCCTGAGCTGAGCTGGCTGGGCCACGAGGGGCTGGGGGAGCCCGCTGTGCTGGGCCGGC TGCGGGAGGACGAGGGCACCTGGGTGCAGGTGTCACTGCTGCACTTCGTGCCCACGAGGGAGGCCAACGG CCACAGGCTGGGCTGCCAGGCCTCCTTCCCCAACACCACCCTGCAGTTCGAGGGCTACGCCAGCATGGAC GTCAAGTACCCCCCGGTGATTGTGGAGATGAACTCCTCGGTGGAGGCCATCGAGGGCTCCCACGTGAGCC TGCTCTGTGGGGCTGACAGCAACCCCCCGCCGCTGCTGACCTGGATGCGGGACGGGACAGTCCTCCGGGA GGCGGTGGCCGAGAGCCTGCTCCTGGAGCTGGAGGAGGTGACCCCCGCCGAAGACGGCGTCTATGCCTGC CTGGCCGAGAATGCCTATGGCCAGGACAACCGCACCGTGGGGCTCAGTGTCATGTATGCACCCTGGAAGC CAACAGTGAACGGGACAATGGTGGCCGTAGAGGGGGAGACGGTCTCTATCTTGTGCTCCACACAGAGCAA CCCGGACCCTATTCTCACCATCTTCAAGGAGAAGCAGATCCTGTCCACGGTCATCTACGAGAGCGAGCTG CAGCTGGAGCTGCCGGCCGTGTCACCCGAGGATGATGGAGAGTACTGGTGTGTGGCTGAGAACCAGTATG GCCAGAGGGCCACCGCCTTCAACCTGTCTGTGGAGTTCGCCCCTGTGCTCCTCCTGGAGTCCCACTGCGC GGCAGCCCGAGACACGGTGCAGTGCCTGTGCGTGGTGAAGTCCAACCCGGAGCCGTCCGTGGCCTTTGAG CTGCCATCGCGCAATGTGACCGTGAACGAGAGCGAGCGGGAGTTCGTGTACTCGGAGCGCAGCGGCCTCG TGCTCACCAGCATCCTCACGCTGCGGGGGCAGGCCCAGGCCCCGCCCCGCGTCATCTGCACCGCGAGGAA CCTCTATGGCGCCAAGAGCCTGGAGCTGCCCTTCCAGGGAGCCCATCGACTGATGTGGGCCAAGATCGGG CCTGTGGGCGCCGTGGTCGCCTTTGCCATCCTGATTGCCATCGTCTGCTACATTACCCAGACACGCAGGA AAAAGAACGTGACAGAGAGCCCCAGCTTCTCGGCAGGGGACAACCCTCCCGTCCTGTTCAGCAGCGACTT CCGCATCTCTGGGGCACCAGAGAAGTACGAGAGCGAGAGGCGCCTGGGATCTGAGAGGAGGCTGCTGGGC CTTCGGGGTGAGCCCCCAGAGCTGGACCTGAGCTATTCTCACTCGGACCTGGGGAAACGGCCCACCAAGG ACAGCTACACGCTGACGGAGGAGCTAGCTGAGTATGCTGAAATCCGGGTCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234527 representing NM_001199216 Red=Cloning site Green=Tags(s) MPSSISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDL GLRNCTLLLSNVSPELGGKYYFRGDLGGYNQYTFSEHSVLDIVNTPNIVVPPEVVAGTEVEVSCMVPDNC PELRPELSWLGHEGLGEPAVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQASFPNTTLQFEGYASMD VKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGTVLREAVAESLLLELEEVTPAEDGVYAC LAENAYGQDNRTVGLSVMYAPWKPTVNGTMVAVEGETVSILCSTQSNPDPILTIFKEKQILSTVIYESEL QLELPAVSPEDDGEYWCVAENQYGQRATAFNLSVEFAPVLLLESHCAAARDTVQCLCVVKSNPEPSVAFE LPSRNVTVNESEREFVYSERSGLVLTSILTLRGQAQAPPRVICTARNLYGAKSLELPFQGAHRLMWAKIG PVGAVVAFAILIAIVCYITQTRRKKNVTESPSFSAGDNPPVLFSSDFRISGAPEKYESERRLGSERRLLG LRGEPPELDLSYSHSDLGKRPTKDSYTLTEELAEYAEIRVK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199216
ORF Size	1803 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199216.1, NP_001186145.1
RefSeq Size	2445 bp
RefSeq ORF	1806 bp
Locus ID	4099
UniProt ID	P20916
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs)
MW	66.7 kDa
Gene Summary	The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2010]

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