SEMA4F (NM_001271661) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	M-SEMA; m-Sema-M; PRO2353; S4F; SEMAM; SEMAW
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233014 representing NM_001271661 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGCCTCTGCTGCGCGGCCCCGCCCGGGTCCCGGGCAGCCTACAGCCTCGCCCTTCCCGCTACTGC TGCTGGCGGTGCTGAGCGGCCCGGTATCCGGCCGCGTCCCCCGCTCGGTGCCCAGAACCTCGCTTCCAAT CTCTGAGGCTGACTCCTGTCTCACCCGGTTCGCAGTCCCTCACACATACAATTACTCTGTTCTCCTTGTG GATCCTGCCTCCCACACACTTTATGTTGGCGCCCGGGACACCATCTTCGCTTTATCCCTGCCCTTCTCAG GGGAGAGACCCCGCAGGATTGACTGGATGGTTCCTGAGGCTCACAGACAGAACTGTAGGAAGAAAGGCAA GAAAGAGGGGGACCTCGGGGGCCGGAAGACCCTCCAGCAGAGATGGACGACGTTTTTGAAAGCTGACCTG CTCTGTCCAGGGCCTGAGCATGGCCGGGCCTCCAGTGTCCTGCAGGATGTTGCTGTGCTTCGACCTGAGC TTGGGGCAGGGACTCCCATCTTTTATGGCATCTTTTCTTCCCAGTGGGAGGGGGCTACTATCTCTGCTGT CTGTGCCTTCCGACCACAAGACATTCGGACAGTGCTGAATGGTCCCTTCAGAGAACTAAAACATGACTGC AACAGAGGACTGCCTGTCGTGGACAATGATGTGCCCCAGCCCAGACCTGGAGAGTGCATCACCAACAACA TGAAGCTCCGGCACTTTGGCTCATCTCTCTCCCTGCCTGACCGCGTACTCACCTTCATCCGGGACCACCC ACTCATGGACAGGCCAGTGTTTCCAGCTGATGGCCACCCCCTGCTGGTCACTACAGATACAGCCTATCTC AGAGTCGTGGCCCACAGGGTGACCAGCCTCTCAGGGAAAGAGTATGATGTGCTCTACCTGGGGACAGAGG ATGGACACCTCCACCGAGCAGTGCGGATCGGAGCTCAGCTCAGCGTTCTTGAAGATCTGGCCTTATTCCC AGAGCCACAGCCAGTTGAGAACATGAAATTGTACCACAGCTGGCTCCTGGTTGGCTCCCGTACTGAGGTG ACACAAGTGAATACAACCAACTGTGGCCGTCTCCAGAGCTGCTCAGAGTGCATCCTGGCCCAGGACCCAG TCTGTGCCTGGAGCTTCCGGCTGGATGAGTGTGTGGCCCATGCCGGGGAGCACCGAGGGTTGGTCCAAGA CATAGAGTCAGCAGATGTCTCCTCTTTGTGTCCTAAAGAGCCTGGAGAACGTCCAGTAGTGTTTGAAGTT CCCGTGGCTACAGCTGCGCATGTGGTCTTGCCATGTTCTCCAAGCTCAGCATGGGCATCCTGTGTGTGGC ACCAGCCCAGTGGAGTGACTGCACTCACCCCCCGGCGGGATGGACTGGAGGTGGTGGTGACCCCAGGGGC CATGGGCGCTTATGCCTGTGAATGTCAGGAGGGTGGGGCAGCCCATGTGGTAGCAGCTTACAGCTTGGTA TGGGGCAGCCAGCGAGATGCTCCGAGCCGGGCCCACACAGTGGGGGCGGGACTGGCTGGCTTCTTCTTGG GGATTCTCGCAGCATCCCTGACTCTCATTCTGATTGGTCGGCGTCAGCAGCGACGGCGACAGAGGGAACT TCTGGCTAGAGACAAGGTGGGCCTGGACCTGGGGGCTCCACCTTCTGGGACCACAAGCTACAGCCAAGAC CCTCCCTCCCCCTCTCCTGAAGATGAGCGGTTGCCGCTGGCCCTGGCCAAGAGGGGCAGTGGCTTTGGTG GATTCTCACCACCCTTCCTGCTTGATCCTTGCCCAAGCCCAGCCCACATTCGGCTAACTGGGGCTCCTCT AGCCACATGTGATGAAACATCCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233014 representing NM_001271661 Red=Cloning site Green=Tags(s) MPASAARPRPGPGQPTASPFPLLLLAVLSGPVSGRVPRSVPRTSLPISEADSCLTRFAVPHTYNYSVLLV DPASHTLYVGARDTIFALSLPFSGERPRRIDWMVPEAHRQNCRKKGKKEGDLGGRKTLQQRWTTFLKADL LCPGPEHGRASSVLQDVAVLRPELGAGTPIFYGIFSSQWEGATISAVCAFRPQDIRTVLNGPFRELKHDC NRGLPVVDNDVPQPRPGECITNNMKLRHFGSSLSLPDRVLTFIRDHPLMDRPVFPADGHPLLVTTDTAYL RVVAHRVTSLSGKEYDVLYLGTEDGHLHRAVRIGAQLSVLEDLALFPEPQPVENMKLYHSWLLVGSRTEV TQVNTTNCGRLQSCSECILAQDPVCAWSFRLDECVAHAGEHRGLVQDIESADVSSLCPKEPGERPVVFEV PVATAAHVVLPCSPSSAWASCVWHQPSGVTALTPRRDGLEVVVTPGAMGAYACECQEGGAAHVVAAYSLV WGSQRDAPSRAHTVGAGLAGFFLGILAASLTLILIGRRQQRRRQRELLARDKVGLDLGAPPSGTTSYSQD PPSPSPEDERLPLALAKRGSGFGGFSPPFLLDPCPSPAHIRLTGAPLATCDETSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271661
ORF Size	1845 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271661.2
RefSeq Size	5657 bp
RefSeq ORF	1848 bp
Locus ID	10505
UniProt ID	O95754
Protein Families	Transmembrane
Protein Pathways	Axon guidance
MW	66.9 kDa
Gene Summary	This gene encodes a transmembrane class IV semaphorin family protein, which plays a role in neural development. This gene may be involved in neurogenesis in prostate cancer, the development of neurofibromas, and breast cancer tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

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