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SMYD1 (NM_198274) Human Tagged ORF Clone
CAT＃: RC221269
SMYD1 (Myc-DDK-tagged)-Human SET and MYND domain containing 1 (SMYD1)
ORF Plasmid: tGFP
"NM_198274" in other vectors (6)
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CNY 3,656.00
CNY 4,280.00
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BOP; KMT3D; ZMYND18; ZMYND22 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221269 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAATAGGGAGAATGGAGAACGTGGAGGTCTTCACCGCTGAGGGCAAAGGAAGGGGTCTGAAGGCCA CCAAGGAGTTCTGGGCTGCAGATATCATCTTTGCTGAGCGGGCTTATTCCGCAGTGGTTTTTGACAGCCT TGTTAATTTTGTGTGCCACACCTGCTTCAAGAGGCAGGAGAAGCTCCATCGCTGTGGGCAGTGCAAGTTT GCCCATTACTGCGACCGCACCTGCCAGAAGGATGCTTGGCTGAACCACAAGAATGAATGTTCGGCCATCA AGAGATATGGGAAGGTGCCCAATGAGAACATCAGGCTGGCGGCGCGCATCATGTGGAGGGTGGAGAGAGA AGGCACCGGGCTCACGGAGGGCTGCCTGGTGTCCGTGGACGACTTGCAGAACCACGTGGAGCACTTTGGG GAGGAGGAGCAGAAGGACCTGCGGGTGGACGTGGACACATTCTTGCAGTACTGGCCGCCGCAGAGCCAGC AGTTCAGCATGCAGTACATCTCGCACATCTTCGGAGTGATTAACTGCAACGGTTTTACTCTCAGTGATCA GAGAGGCCTGCAGGCCGTGGGCGTAGGCATCTTCCCCAACCTGGGCCTGGTGAACCATGACTGTTGGCCC AACTGTACTGTCATATTTAACAATGGCAATCATGAGGCAGTGAAATCCATGTTTCATACCCAGATGAGAA TTGAGCTCCGGGCCCTAGGCAAGATCTCAGAAGGAGAGGAGCTGACTGTGTCCTATATTGACTTCCTCAA CGTTAGTGAAGAACGCAAGAGGCAGCTGAAGAAGCAGTACTACTTTGACTGCACATGTGAACACTGCCAG AAAAAACTGAAGGATGACCTCTTCCTGGGGGTGAAAGACAACCCCAAGCCCTCTCAGGAAGTGGTGAAGG AGATGATACAATTCTCCAAGGATACATTGGAAAAGATAGACAAGGCTCGTTCCGAGGGTTTGTATCATGA GGTTGTGAAATTATGCCGGGAGTGCCTGGAGAAGCAGGAGCCAGTGTTTGCTGACACCAACATCTACATG CTGCGGATGCTGAGCATTGTTTCGGAGGTCCTTTCCTACCTCCAGGCCTTTGAGGAGGCCTCGTTCTATG CCAGGAGGATGGTGGACGGCTATATGAAGCTCTACCACCCCAACAATGCCCAACTGGGCATGGCCGTGAT GCGGGCAGGGCTGACCAACTGGCATGCTGGTAACATTGAGGTGGGGCACGGGATGATCTGCAAAGCCTAT GCCATTCTCCTGGTGACACACGGACCCTCCCACCCCATCACTAAGGACTTAGAGGCCATGCGGGTGCAGA CGGAGATGGAGCTACGCATGTTCCGCCAGAACGAATTCATGTACTACAAGATGCGCGAGGCTGCCCTGAA CAACCAGCCCATGCAGGTCATGGCCGAGCCCAGCAATGAGCCATCCCCAGCTCTGTTCCACAAGAAGCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221269 protein sequence
Red=Cloning site Green=Tags(s) MTIGRMENVEVFTAEGKGRGLKATKEFWAADIIFAERAYSAVVFDSLVNFVCHTCFKRQEKLHRCGQCKF AHYCDRTCQKDAWLNHKNECSAIKRYGKVPNENIRLAARIMWRVEREGTGLTEGCLVSVDDLQNHVEHFG EEEQKDLRVDVDTFLQYWPPQSQQFSMQYISHIFGVINCNGFTLSDQRGLQAVGVGIFPNLGLVNHDCWP NCTVIFNNGNHEAVKSMFHTQMRIELRALGKISEGEELTVSYIDFLNVSEERKRQLKKQYYFDCTCEHCQ KKLKDDLFLGVKDNPKPSQEVVKEMIQFSKDTLEKIDKARSEGLYHEVVKLCRECLEKQEPVFADTNIYM LRMLSIVSEVLSYLQAFEEASFYARRMVDGYMKLYHPNNAQLGMAVMRAGLTNWHAGNIEVGHGMICKAY AILLVTHGPSHPITKDLEAMRVQTEMELRMFRQNEFMYYKMREAALNNQPMQVMAEPSNEPSPALFHKKQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198274 |
| ORF Size | 1470 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_198274.4 |
| RefSeq Size | 4441 bp |
| RefSeq ORF | 1473 bp |
| Locus ID | 150572 |
| UniProt ID | Q8NB12 |
| Protein Families | Druggable Genome |
| MW | 56.6 kDa |
| Gene Summary | Methylates histone H3 at 'Lys-4' (H3K4me), seems able to perform both mono-, di-, and trimethylation. Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221269L1 | Lenti ORF clone of Human SET and MYND domain containing 1 (SMYD1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC221269L2 | Lenti ORF clone of Human SET and MYND domain containing 1 (SMYD1), mGFP tagged |
CNY 6,180.00 |
|
| RC221269L3 | Lenti ORF clone of Human SET and MYND domain containing 1 (SMYD1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC221269L4 | Lenti ORF clone of Human SET and MYND domain containing 1 (SMYD1), mGFP tagged |
CNY 6,056.00 |
|
| RG221269 | SMYD1 (tGFP-tagged) - Human SET and MYND domain containing 1 (SMYD1) |
CNY 4,750.00 |
|
| SC307665 | SMYD1 (untagged)-Human SET and MYND domain containing 1 (SMYD1) |
CNY 3,656.00 |
