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EBF2 (NM_022659) Human Tagged ORF Clone
CAT＃: RC220630
EBF2 (Myc-DDK-tagged)-Human early B-cell factor 2 (EBF2)
ORF Plasmid: tGFP
"NM_022659" in other vectors (6)
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CNY 6,424.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | COE2; EBF-2; O/E-3; OE-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC220630 representing NM_022659
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGGAATTCAAGATACTTTAGGAAGAGGACCAACTCTGAAAGAGAAATCGCTGGGCGCGGAGATGG ATTCGGTCAGGTCCTGGGTCCGGAATGTCGGAGTGGTGGACGCTAATGTCGCCGCGCAGAGCGGGGTCGC CCTGTCCCGGGCCCACTTTGAGAAACAGCCTCCTTCCAACTTGAGGAAATCCAACTTCTTTCACTTCGTC CTGGCGCTCTATGACAGGCAGGGCCAGCCGGTGGAGATCGAGCGGACGGCCTTCGTGGACTTTGTGGAGA ATGACAAAGAACAAGGCAACGAGAAGACCAACAACGGCACTCACTACAAGTTACAGCTCCTCTACAGCAA CGGTGTCCGCACGGAACAGGACCTCTATGTCAGGCTCATCGACTCGGTCACCAAGCAGCCCATCGCTTAC GAGGGACAGAATAAGAATCCGGAAATGTGCCGAGTTCTCCTGACGCACGAAGTGATGTGTAGTCGATGCT GCGAAAAGAAAAGCTGTGGAAACCGAAATGAGACTCCATCGGACCCAGTCATAATTGACAGATTCTTTTT AAAATTTTTCCTCAAGTGCAATCAGAATTGTTTGAAAACAGCAGGAAACCCAAGGGACATGAGACGGTTT CAGGTTGTGTTGTCAACAACGGTGAATGTGGATGGACACGTCCTGGCTGTTTCTGACAACATGTTTGTTC ATAACAACTCCAAGCATGGACGGAGAGCAAGAAGACTCGATCCATCGGAAGCTACCCCCTGCATCAAAGC CATTAGCCCGAGTGAAGGCTGGACCACAGGAGGAGCCATGGTCATCATCATCGGGGACAACTTCTTTGAT GGTCTCCAAGTGGTGTTTGGGACTATGCTTGTATGGAGCGAGCTAATAACCCCTCATGCCATCAGAGTAC AGACTCCTCCCCGGCACATCCCAGGCGTGGTAGAGGTGACATTATCTTATAAATCTAAACAGTTCTGCAA AGGAGCCCCAGGAAGGTTCATTTACACAGCATTAAATGAACCCACCATAGACTATGGCTTCCAGAGACTG CAGAAGGTCATCCCTAGGCATCCTGGAGATCCTGAGAGATTAGCTAAGGAGATGCTGTTGAAAAGAGCTG CAGATCTAGTGGAAGCTCTTTATGGCACACCACACAATAACCAGGACATCATTTTGAAGCGAGCCGCAGA CATTGCTGAAGCTCTCTACAGCGTCCCCAGGAATCCCAGCCAGCTTCCAGCCCTCTCTAGCTCCCCAGCG CACAGTGGCATGATGGGAATCAACTCCTATGGCAGCCAGCTTGGGGTCAGCATCTCAGAGTCAACACAAG GAAATAATCAAGGGTACATCCGCAACACAAGCAGCATCTCTCCGCGGGGATACTCTTCCAGCTCCACGCC TCAACAGTCTAATTACAGTACCTCCAGCAACAGTATGAATGGCTACAGCAATGTCCCCATGGCCAACTTG GGTGTTCCAGGTTCACCAGGATTTCTAAATGGCTCACCCACCGGCTCTCCTTATGGAATCATGTCATCAA GTCCCACCGTTGGGTCTTCCAGCACATCCTCCATCCTCCCATTTTCCTCTTCAGTTTTTCCTGCTGTCAA ACAGAAGAGTGCCTTTGCCCCTGTCATCAGGCCCCAAGGCTCCCCTTCACCTGCCTGCTCCAGCGGCAAT GGAAATGGATTCAGAGCCATGACCGGACTTGTTGTACCCCCGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220630 representing NM_022659
Red=Cloning site Green=Tags(s) MFGIQDTLGRGPTLKEKSLGAEMDSVRSWVRNVGVVDANVAAQSGVALSRAHFEKQPPSNLRKSNFFHFV LALYDRQGQPVEIERTAFVDFVENDKEQGNEKTNNGTHYKLQLLYSNGVRTEQDLYVRLIDSVTKQPIAY EGQNKNPEMCRVLLTHEVMCSRCCEKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKTAGNPRDMRRF QVVLSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEATPCIKAISPSEGWTTGGAMVIIIGDNFFD GLQVVFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGAPGRFIYTALNEPTIDYGFQRL QKVIPRHPGDPERLAKEMLLKRAADLVEALYGTPHNNQDIILKRAADIAEALYSVPRNPSQLPALSSSPA HSGMMGINSYGSQLGVSISESTQGNNQGYIRNTSSISPRGYSSSSTPQQSNYSTSSNSMNGYSNVPMANL GVPGSPGFLNGSPTGSPYGIMSSSPTVGSSSTSSILPFSSSVFPAVKQKSAFAPVIRPQGSPSPACSSGN GNGFRAMTGLVVPPM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022659 |
| ORF Size | 1725 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022659.4 |
| RefSeq Size | 2297 bp |
| RefSeq ORF | 1728 bp |
| Locus ID | 64641 |
| UniProt ID | Q9HAK2 |
| MW | 62.5 kDa |
| Gene Summary | The protein encoded by this gene belongs to the COE (Collier/Olf/EBF) family of non-basic, helix-loop-helix transcription factors that have a well conserved DNA binding domain. The COE family proteins play an important role in variety of developmental processes. Studies in mouse suggest that this gene may be involved in the differentiation of osteoblasts. [provided by RefSeq, Oct 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220630L1 | Lenti ORF clone of Human early B-cell factor 2 (EBF2), Myc-DDK-tagged |
CNY 8,824.00 |
|
| RC220630L2 | Lenti ORF clone of Human early B-cell factor 2 (EBF2), mGFP tagged |
CNY 6,460.00 |
|
| RC220630L3 | Lenti ORF clone of Human early B-cell factor 2 (EBF2), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC220630L4 | Lenti ORF clone of Human early B-cell factor 2 (EBF2), mGFP tagged |
CNY 6,460.00 |
|
| RG220630 | EBF2 (tGFP-tagged) - Human early B-cell factor 2 (EBF2) |
CNY 8,024.00 |
|
| SC317986 | EBF2 (untagged)-Human early B-cell factor 2 (EBF2) |
CNY 6,432.00 |
