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Glycine Receptor alpha 3 (GLRA3) (NM_001042543) Human Tagged ORF Clone
CAT＃: RC219102
- TrueORF®
GLRA3 (Myc-DDK-tagged)-Human glycine receptor, alpha 3 (GLRA3), transcript variant 2
ORF Plasmid: tGFP
"NM_001042543" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219102 representing NM_001042543
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCACGTGAGACACTTTCGGACATTAGTTTCGGGATTTTACTTCTGGGAAGCAGCACTGTTACTCA GTTTGGTTGCCACAAAGGAAACAGACAGTGCAAGATCTCGAAGTGCTCCAATGTCACCTTCTGATTTTCT GGATAAATTAATGGGCAGGACATCAGGATATGATGCAAGAATCAGACCCAATTTTAAAGGCCCTCCAGTT AATGTCACATGCAACATATTCATCAACAGCTTTGGCTCTATCGCAGAGACGACCATGGATTACAGAGTGA ATATCTTTCTTCGTCAGAAATGGAATGATCCCCGCCTCGCGTACAGTGAATATCCTGACGACTCTTTAGA CCTCGACCCCTCCATGTTGGACTCCATTTGGAAACCTGATTTGTTCTTTGCCAATGAAAAGGGTGCCAAC TTTCATGAAGTCACTACAGACAACAAATTGCTAAGAATTTTCAAAAATGGAAATGTTCTTTATTCAATAA GATTAACATTAACACTTTCCTGTCCAATGGATCTCAAGAATTTTCCCATGGATGTACAAACATGTATAAT GCAACTGGAAAGCTTTGGGTACACAATGAATGATCTCATTTTTGAATGGCAAGATGAGGCACCCGTACAA GTGGCAGAAGGACTCACTTTGCCCCAGTTTCTGTTGAAAGAAGAAAAAGATTTACGATACTGCACTAAAC ATTACAATACAGGAAAGTTTACGTGTATAGAAGTGCGATTCCATCTGGAGCGACAAATGGGATACTATCT GATCCAGATGTACATTCCCAGTCTCCTGATTGTTATTCTATCCTGGGTTTCATTCTGGATCAACATGGAT GCAGCACCGGCCAGGGTAGCTCTGGGGATAACCACTGTGCTAACGATGACTACACAGAGTTCAGGATCAC GAGCTTCCTTGCCAAAAGTTTCATATGTCAAAGCTATTGATATTTGGATGGCAGTATGCCTCCTTTTTGT GTTTTCAGCACTTCTGGAGTATGCAGCTGTAAATTTTGTATCAAGACAACACAAAGAACTTCTGAGATTT CGACGAAAGAGAAAGAATAAGGATGATGAGGTAAGGGAAAGCCGATTCAGCTTCACAGCCTATGGAATGG GACCATGTCTACAAGCAAAGGATGGCATGACTCCAAAGGGCCCCAACCACCCTGTCCAGGTAATGCCAAA AAGTCCTGATGAAATGAGGAAGGTCTTTATCGACCGGGCCAAGAAGATTGATACCATCTCCCGAGCCTGC TTCCCATTAGCTTTTTTGATTTTTAATATTTTCTACTGGGTTATCTATAAAATTCTTAGGCATGAGGATA TTCATCAGCAGCAAGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219102 representing NM_001042543
Red=Cloning site Green=Tags(s) MAHVRHFRTLVSGFYFWEAALLLSLVATKETDSARSRSAPMSPSDFLDKLMGRTSGYDARIRPNFKGPPV NVTCNIFINSFGSIAETTMDYRVNIFLRQKWNDPRLAYSEYPDDSLDLDPSMLDSIWKPDLFFANEKGAN FHEVTTDNKLLRIFKNGNVLYSIRLTLTLSCPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQDEAPVQ VAEGLTLPQFLLKEEKDLRYCTKHYNTGKFTCIEVRFHLERQMGYYLIQMYIPSLLIVILSWVSFWINMD AAPARVALGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRF RRKRKNKDDEVRESRFSFTAYGMGPCLQAKDGMTPKGPNHPVQVMPKSPDEMRKVFIDRAKKIDTISRAC FPLAFLIFNIFYWVIYKILRHEDIHQQQD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001042543 |
| ORF Size | 1347 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001042543.3 |
| RefSeq Size | 3592 bp |
| RefSeq ORF | 1350 bp |
| Locus ID | 8001 |
| UniProt ID | O75311 |
| Protein Families | Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 51.8 kDa |
| Gene Summary | This gene encodes a member of the ligand-gated ion channel protein family. The encoded protein is a member of the glycine receptor subfamily. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219102L3 | Lenti-ORF clone of GLRA3 (Myc-DDK-tagged)-Human glycine receptor, alpha 3 (GLRA3), transcript variant 2 |
CNY 5,890.00 |
|
| RC219102L4 | Lenti-ORF clone of GLRA3 (mGFP-tagged)-Human glycine receptor, alpha 3 (GLRA3), transcript variant 2 |
CNY 5,890.00 |
|
| RG219102 | GLRA3 (tGFP-tagged) - Human glycine receptor, alpha 3 (GLRA3), transcript variant 2 |
CNY 4,370.00 |
|
| SC311380 | GLRA3 (untagged)-Human glycine receptor, alpha 3 (GLRA3), transcript variant 2 |
CNY 5,488.00 |
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