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DEGS2 (NM_206918) Human Tagged ORF Clone
CAT＃: RC217308
DEGS2 (Myc-DDK-tagged)-Human degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) (DEGS2)
ORF Plasmid: tGFP
"NM_206918" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C14orf66; DES2; FADS8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217308 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAACAGCGCGAGCCGCAGCGACTTCGAGTGGGTCTACACCGACCAGCCGCACACGCAGCGGCGCA AGGAGATACTGGCCAAGTACCCGGCCATCAAGGCCCTGATGCGGCCAGACCCGCGCCTCAAGTGGGCGGT GCTGGTGCTGGTGCTGGTGCAGATGCTGACCTGCTGGCTGGTGCGCGGGCTGGCCTGGCGCTGGCTGCTG TTCTGGGCCTACGCCTTTGGTGGCTGCGTGAACCACTCGCTGACGCTGGCCATCCACGACATCTCGCACA ACGCGGCCTTCGGCACGGGCCGTGCGGCACGCAACCGCTGGCTGGCCGTGTTCGCCAACCTGCCCGTGGG TGTGCCCTACGCCGCCTCCTTCAAGAAGTACCACGTGGACCACCACCGCTACCTGGGCGGCGACGGGCTG GACGTGGACGTGCCCACGCGTCTGGAGGGCTGGTTCTTCTGCACGCCCGCCCGCAAGCTGCTCTGGCTGG TGCTGCAGCCCTTCTTCTACTCACTACGGCCGCTCTGCGTCCACCCCAAGGCCGTGACCCGCATGGAGGT GCTCAACACGCTGGTGCAGCTGGCGGCCGACCTGGCCATCTTTGCCCTTTGGGGGCTCAAGCCCGTGGTC TACCTGCTGGCCAGCTCCTTCCTGGGCCTGGGCCTGCACCCCATCTCGGGCCACTTCGTGGCCGAGCACT ACATGTTCCTCAAGGGCCACGAGACCTACTCCTACTATGGGCCTCTCAACTGGATCACCTTCAATGTGGG CTACCACGTGGAGCACCACGACTTCCCCAGCATCCCGGGCTACAACCTGCCGCTGGTGCGGAAGATCGCG CCCGAGTACTACGACCACCTGCCGCAGCACCACTCCTGGGTGAAGGTGCTCTGGGATTTTGTGTTTGAGG ACTCCCTGGGGCCCTATGCCAGGGTGAAGCGGGTGTACAGGCTGGCAAAAGATGGTCTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC217308 protein sequence
Red=Cloning site Green=Tags(s) MGNSASRSDFEWVYTDQPHTQRRKEILAKYPAIKALMRPDPRLKWAVLVLVLVQMLTCWLVRGLAWRWLL FWAYAFGGCVNHSLTLAIHDISHNAAFGTGRAARNRWLAVFANLPVGVPYAASFKKYHVDHHRYLGGDGL DVDVPTRLEGWFFCTPARKLLWLVLQPFFYSLRPLCVHPKAVTRMEVLNTLVQLAADLAIFALWGLKPVV YLLASSFLGLGLHPISGHFVAEHYMFLKGHETYSYYGPLNWITFNVGYHVEHHDFPSIPGYNLPLVRKIA PEYYDHLPQHHSWVKVLWDFVFEDSLGPYARVKRVYRLAKDGL SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_206918 |
| ORF Size | 969 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_206918.1, NP_996801.1 |
| RefSeq Size | 1411 bp |
| RefSeq ORF | 972 bp |
| Locus ID | 123099 |
| UniProt ID | Q6QHC5 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, Sphingolipid metabolism |
| MW | 37.2 kDa |
| Gene Summary | This gene encodes a bifunctional enzyme that is involved in the biosynthesis of phytosphingolipids in human skin and in other phytosphingolipid-containing tissues. This enzyme can act as a sphingolipid delta(4)-desaturase, and also as a sphingolipid C4-hydroxylase. [provided by RefSeq, Oct 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Dihydroceramide-based Response to Hypoxia *
,null,
The Journal of Biological Chemistry
,PubMed ID 21914808
[DEGS2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217308L1 | Lenti ORF clone of Human degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) (DEGS2), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC217308L2 | Lenti ORF clone of Human degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) (DEGS2), mGFP tagged |
CNY 5,890.00 |
|
| RC217308L3 | Lenti ORF clone of Human degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) (DEGS2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217308L4 | Lenti ORF clone of Human degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) (DEGS2), mGFP tagged |
CNY 5,890.00 |
|
| RG217308 | DEGS2 (tGFP-tagged) - Human degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) (DEGS2) |
CNY 4,000.00 |
|
| SC124595 | DEGS2 (untagged)-Human degenerative spermatocyte homolog 2, lipid desaturase (Drosophila) (DEGS2) |
CNY 2,400.00 |
