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A1CF (NM_138933) Human Tagged ORF Clone
CAT＃: RC216911
A1CF (Myc-DDK-tagged)-Human APOBEC1 complementation factor (A1CF), transcript variant 3
ORF Plasmid: tGFP
"NM_138933" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ACF; ACF64; ACF65; APOBEC1CF; ASP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216911 representing NM_138933
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGCAGTGTGTCTGGGCACATGCCCAGAGCCAGAAGCGAGCATGAGCACAGCAATTCCTGGCCTCA AAAAAGGAAATAATGCTTTGCAGTCCATCATTTTGCAAACTTTGCTGGAAAAGGAAAATGGACAAAGAAA ATATGGTGGCCCTCCACCTGGTTGGGATGCTGCACCCCCTGAAAGGGGCTGTGAAATTTTTATTGGAAAA CTTCCCCGAGACCTTTTTGAGGATGAGCTTATACCATTATGTGAAAAAATCGGTAAAATTTATGAAATGA GAATGATGATGGATTTTAATGGCAACAATAGAGGATATGCATTTGTAACATTTTCAAATAAAGTGGAAGC CAAGAATGCAATCAAGCAACTTAATAATTATGAAATTAGAAATGGGCGCCTCTTAGGGGTTTGTGCCAGT GTGGACAACTGCCGATTATTTGTTGGGGGCATCCCAAAAACCAAAAAGAGAGAAGAAATCTTATCGGAGA TGAAAAAGGTTACTGAAGGTGTTGTCGATGTCATCGTCTACCCAAGCGCTGCAGATAAAACCAAAAACCG AGGCTTTGCCTTCGTGGAGTATGAGAGTCATCGAGCAGCTGCCATGGCGAGGAGGAAACTGCTACCAGGA AGAATTCAGTTATGGGGACATGGTATTGCAGTAGACTGGGCAGAGCCAGAAGTAGAAGTTGATGAAGATA CAATGTCTTCAGTGAAAATCCTATATGTAAGAAATCTTATGCTGTCTACCTCTGAAGAGATGATTGAAAA GGAATTCAACAATATCAAACCAGGTGCTGTGGAGAGGGTGAAGAAAATTCGAGACTATGCTTTTGTGCAC TTCAGTAACCGAGAAGATGCAGTTGAGGCTATGAAAGCTTTAAATGGCAAGGTGCTGGATGGTTCCCCCA TTGAAGTCACCCTAGCAAAACCAGTGGACAAGGACAGTTATGTTAGGTATACCCGAGGCACAGGTGGAAG GGGCACCATGCTGCAAGGAGAGTATACCTACTCTTTGGGCCAAGTTTATGATCCCACCACAACCTACCTT GGAGCTCCTGTCTTCTATGCCCCCCAGACCTATGCAGCAATTCCCAGTCTTCATTTCCCAGCCACCAAAG GACATCTCAGCAACAGAGCCATTATCCGAGCCCCTTCTGTTAGAGGGGCTGCGGGAGTGAGAGGACTGGG CGGCCGTGGCTATTTGGCATACACAGGCCTGGGTCGAGGATACCAGGTCAAAGGAGACAAAAGAGAAGAC AAACTCTATGACATTTTACCTGGGATGGAGCTCACCCCAATGAATCCTGTCACATTAAAACCCCAAGGAA TTAAACTCGCTCCCCAGATATTAGAAGAGATTTGTCAGAAAAATAACTGGGGACAGCCAGTGTACCAGCT GCACTCTGCTATTGGACAAGACCAAAGACAGCTATTCTTGTACAAAATAACTATTCCTGCTCTAGCCAGC CAGAATCCTGCAATCCACCCTTTCACACCTCCAAAGCTGAGTGCCTTTGTGGATGAAGCAAAGACGTATG CAGCCGAATACACCCTGCAGACCCTGGGCATCCCCACTGATGGAGGCGATGGCACCATGGCTACTGCTGC TGCTGCTGCTACTGCTTTCCCAGGATATGCTGTCCCTAATGCAACTGCACCCGTGTCTGCAGCCCAGCTC AAGCAAGCGGTAACCCTTGGACAAGACTTAGCAGCATATACAACCTATGAGGTCTACCCAACTTTTGCAG TGACTGCCCGAGGGGATGGATATGGCACCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216911 representing NM_138933
Red=Cloning site Green=Tags(s) MEAVCLGTCPEPEASMSTAIPGLKKGNNALQSIILQTLLEKENGQRKYGGPPPGWDAAPPERGCEIFIGK LPRDLFEDELIPLCEKIGKIYEMRMMMDFNGNNRGYAFVTFSNKVEAKNAIKQLNNYEIRNGRLLGVCAS VDNCRLFVGGIPKTKKREEILSEMKKVTEGVVDVIVYPSAADKTKNRGFAFVEYESHRAAAMARRKLLPG RIQLWGHGIAVDWAEPEVEVDEDTMSSVKILYVRNLMLSTSEEMIEKEFNNIKPGAVERVKKIRDYAFVH FSNREDAVEAMKALNGKVLDGSPIEVTLAKPVDKDSYVRYTRGTGGRGTMLQGEYTYSLGQVYDPTTTYL GAPVFYAPQTYAAIPSLHFPATKGHLSNRAIIRAPSVRGAAGVRGLGGRGYLAYTGLGRGYQVKGDKRED KLYDILPGMELTPMNPVTLKPQGIKLAPQILEEICQKNNWGQPVYQLHSAIGQDQRQLFLYKITIPALAS QNPAIHPFTPPKLSAFVDEAKTYAAEYTLQTLGIPTDGGDGTMATAAAAATAFPGYAVPNATAPVSAAQL KQAVTLGQDLAAYTTYEVYPTFAVTARGDGYGTF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138933 |
| ORF Size | 1782 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138933.2, NP_620311.1 |
| RefSeq Size | 2223 bp |
| RefSeq ORF | 1785 bp |
| Locus ID | 29974 |
| UniProt ID | Q9NQ94 |
| MW | 64.8 kDa |
| Gene Summary | Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216911L3 | Lenti ORF clone of Human APOBEC1 complementation factor (A1CF), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216911L4 | Lenti ORF clone of Human APOBEC1 complementation factor (A1CF), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG216911 | A1CF (tGFP-tagged) - Human APOBEC1 complementation factor (A1CF), transcript variant 3 |
CNY 4,370.00 |
|
| SC306093 | A1CF (untagged)-Human APOBEC1 complementation factor (A1CF), transcript variant 3 |
CNY 7,220.00 |
