C14orf130 (UBR7) (NM_001100417) Human Tagged ORF Clone
CAT#: RC215315
UBR7 (Myc-DDK-tagged)-Human ubiquitin protein ligase E3 component n-recognin 7 (putative) (UBR7), transcript variant 3
ORF Plasmid: tGFP
"NM_001100417" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C14orf130; FLJ10483; MGC9518 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC215315 representing NM_001100417
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCGTGCGCTGTCCTGGGCGGCAGCGACTCCGAGAAGTGCTCCTACTCTCAGAAATTTTCGTTGTG ATTGTGGAAACAGCAAGTTTAAAAATTTGGAATGCAAATTACTTCCTGACAAAGCAAAGGTAAATTCTGG CAATAAGTACAATGACAACTTTTTTGGATTGTACTGCATTTGCAAGAGACCTTATCCTGATCCTGAAGAC GAGATTCCAGATGAGATGATCCAGTGCGTAGTCTGTGAAGACTGGTTCCATGGAAGGCATCTTGGTGCCA TTCCCCCTGAGAGTGGGGATTTTCAGGAGATGGTATGCCAGGCCTGCATGAAACGTTGTTCTTTTTTGTG GGCTTATGCTGCACAATTGGCAGTAACCAAAATATCCACTGAGGATGATGGATTGGTGCGGAACATTGAT GGAATAGGTGATCAGGAAGTTATCAAACCTGAAAATGGAGAGCATCAAGATAGTACCCTCAAAGAGGATG TTCCAGAACAGGGAAAGGATGATGTCCGGGAGGTTAAAGTAGAGCAGAACAGTGAACCATGTGCCGGCTC TAGTTCTGAATCTGATCTCCAGACAGTGTTTAAGAATGAAAGCCTCAACGCAGAATCAAAATCTGGCTGC AAACTTCAGGAGCTTAAAGCTAAGCAGCTTATAAAGAAAGACACTGCCACCTATTGGCCCCTGAACTGGC GTAGCAAGTTGTGTACCTGCCAAGACTGTATGAAAATGTATGGAGATCTAGATGTCTTATTCCTGACAGA TGAATACGACACAGTTCTGGCTTATGAAAACAAAGGGAAGATTGCCCAGGCCACTGACAGGAGCGATCCC CTAATGGATACCCTTAGCAGCATGAATAGAGTCCAGCAAGTGGAACTCATTTGTGAATACAATGATTTGA AGACTGAACTTAAAGACTATCTCAAGAGATTTGCTGATGAAGGCACGGTTGTTAAGAGAGAGGACATTCA GCAGTTCTTTGAAGAGTTTCAGTCAAAAAAGAGAAGAAGAGTGGATGGGATGCAGTATTACTGCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215315 representing NM_001100417
Red=Cloning site Green=Tags(s) MRRALSWAAATPRSAPTLRNFRCDCGNSKFKNLECKLLPDKAKVNSGNKYNDNFFGLYCICKRPYPDPED EIPDEMIQCVVCEDWFHGRHLGAIPPESGDFQEMVCQACMKRCSFLWAYAAQLAVTKISTEDDGLVRNID GIGDQEVIKPENGEHQDSTLKEDVPEQGKDDVREVKVEQNSEPCAGSSSESDLQTVFKNESLNAESKSGC KLQELKAKQLIKKDTATYWPLNWRSKLCTCQDCMKMYGDLDVLFLTDEYDTVLAYENKGKIAQATDRSDP LMDTLSSMNRVQQVELICEYNDLKTELKDYLKRFADEGTVVKREDIQQFFEEFQSKKRRRVDGMQYYCS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001100417 |
ORF Size | 1047 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001100417.1, NP_001093887.1 |
RefSeq Size | 3575 bp |
RefSeq ORF | 1049 bp |
Locus ID | 55148 |
Protein Families | Druggable Genome |
MW | 39.8 kDa |
Gene Summary | This gene encodes a UBR box-containing protein that belongs to the E3 ubiquitin ligase family. The protein also contains a plant homeodomain (PHD) in the C-terminus. In mammals, the encoded protein recognizes N-degrons, the destabilizing N-terminal residues of short-lived proteins, which results in ubiquitinylation, and proteolysis via the proteasome. [provided by RefSeq, Jul 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215315L1 | Lenti ORF clone of Human ubiquitin protein ligase E3 component n-recognin 7 (putative) (UBR7), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC215315L2 | Lenti ORF clone of Human ubiquitin protein ligase E3 component n-recognin 7 (putative) (UBR7), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
RC215315L3 | Lenti ORF clone of Human ubiquitin protein ligase E3 component n-recognin 7 (putative) (UBR7), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC215315L4 | Lenti ORF clone of Human ubiquitin protein ligase E3 component n-recognin 7 (putative) (UBR7), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
RG215315 | UBR7 (tGFP-tagged) - Human ubiquitin protein ligase E3 component n-recognin 7 (putative) (UBR7), transcript variant 3 |
CNY 4,370.00 |
|
SC316729 | UBR7 (untagged)-Human ubiquitin protein ligase E3 component n-recognin 7 (putative) (UBR7), transcript variant 3 |
CNY 5,700.00 |
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