AP3M1 (NM_207012) Human Tagged ORF Clone

CAT#: RC214850

AP3M1 (Myc-DDK-tagged)-Human adaptor-related protein complex 3, mu 1 subunit (AP3M1), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_207012" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal AP3M1 Antibody
    • 100 ug

CNY 5,808.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC214850 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCCACAGTCTATTTCTCATAAACTGTTCCGGTGACATATTTCTAGAGAAGCACTGGAAGAGCGTTG
TGAGCCAGTCTGTCTGTGATTATTTCTTTGAAGCTCAAGAGAAAGCTGCTGATGTTGAAAATGTACCACC
TGTCATTTCAACACCTCACCACTACCTCATCAGTATCTACCGGGATAAGCTCTTCTTTGTATCTGTCATA
CAGACCGAAGTGCCACCTCTCTTTGTAATTGAGTTCCTACATCGAGTTGCTGACACTTTTCAGGACTACT
TTGGTGAGTGTTCAGAGGCTGCAATTAAGGATAATGTGGTCATAGTATATGAACTCTTAGAAGAAATGTT
AGACAATGGATTTCCACTGGCTACCGAATCTAACATTTTGAAAGAATTGATTAAACCACCAACAATTCTA
CGCTCTGTTGTCAACTCTATTACAGGCAGTAGTAATGTTGGGGACACACTCCCCACCGGGCAGCTGTCCA
ACATACCATGGCGTCGGGCAGGGGTAAAGTACACAAACAATGAAGCCTATTTTGATGTTGTTGAAGAAAT
AGACGCAATTATAGATAAATCAGGATCTACAGTCTTTGCAGAAATTCAGGGGGTCATTGATGCTTGCATT
AAACTATCTGGAATGCCTGATCTCTCCCTTTCTTTCATGAACCCTAGGCTTCTGGATGATGTCAGCTTTC
ACCCCTGCATCCGGTTCAAGCGTTGGGAATCTGAAAGAGTTTTGTCATTTATTCCTCCAGATGGAAATTT
CCGACTCATATCATACCGTGTCAGCTCACAAAATCTAGTGGCAATACCAGTGTATGTGAAACATAGTATC
AGCTTTAAGGAGAACAGTTCTTGCGGCAGATTTGATATAACAATTGGACCAAAGCAGAATATGGGGAAAA
CTATTGAAGGAATTACAGTGACAGTTCACATGCCAAAAGTTGTGCTGAACATGAACCTGACACCCACACA
AGGCAGCTATACATTTGATCCAGTCACCAAGGTACTAACATGGGATGTGGGAAAAATTACTCCACAAAAG
CTCCCAAGTCTTAAAGGACTGGTAAATTTACAGTCTGGAGCCCCCAAACCAGAAGAGAATCCGAGCCTCA
ACATACAGTTTAAGATCCAGCAGCTTGCTATTTCAGGCTTAAAAGTAAACCGTTTGGACATGTATGGGGA
GAAATATAAGCCATTTAAAGGAGTCAAATACGTCACGAAAGCTGGAAAGTTCCAAGTGAGGACA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC214850 protein sequence
Red=Cloning site Green=Tags(s)

MIHSLFLINCSGDIFLEKHWKSVVSQSVCDYFFEAQEKAADVENVPPVISTPHHYLISIYRDKLFFVSVI
QTEVPPLFVIEFLHRVADTFQDYFGECSEAAIKDNVVIVYELLEEMLDNGFPLATESNILKELIKPPTIL
RSVVNSITGSSNVGDTLPTGQLSNIPWRRAGVKYTNNEAYFDVVEEIDAIIDKSGSTVFAEIQGVIDACI
KLSGMPDLSLSFMNPRLLDDVSFHPCIRFKRWESERVLSFIPPDGNFRLISYRVSSQNLVAIPVYVKHSI
SFKENSSCGRFDITIGPKQNMGKTIEGITVTVHMPKVVLNMNLTPTQGSYTFDPVTKVLTWDVGKITPQK
LPSLKGLVNLQSGAPKPEENPSLNIQFKIQQLAISGLKVNRLDMYGEKYKPFKGVKYVTKAGKFQVRT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_207012
ORF Size 1254 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_207012.4
RefSeq Size 5270 bp
RefSeq ORF 1257 bp
Locus ID 26985
UniProt ID Q9Y2T2
Protein Pathways Lysosome
MW 46.9 kDa
Gene Summary The protein encoded by this gene is the medium subunit of AP-3, which is an adaptor-related protein complex associated with the Golgi region as well as more peripheral intracellular structures. AP-3 facilitates the budding of vesicles from the Golgi membrane, and it may directly function in protein sorting to the endosomal/lysosomal system. AP-3 is a heterotetrameric protein complex composed of two large subunits (delta and beta3), a medium subunit (mu3), and a small subunit (sigma 3). Mutations in one of the large subunits of AP-3 have been associated with the Hermansky-Pudlak syndrome, a genetic disorder characterized by defective lysosome-related organelles. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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