Neuronal membrane glycoprotein M6 a (GPM6A) (NM_201592) Human Tagged ORF Clone

CAT#: RC213529

GPM6A (Myc-DDK-tagged)-Human glycoprotein M6A (GPM6A), transcript variant 3

ORF Plasmid: DDK tGFP



  "NM_201592" in other vectors (4)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


GPM6A Antibody - C-terminal region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms GPM6; M6A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC213529 representing NM_201592
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGTGTTTTGAATGCTGTATCAAATGCCTGGGGGGCATTCCCTATGCCTCTCTGATTGCCACCATCC
TGCTCTATGCGGGTGTTGCCCTGTTCTGTGGCTGCGGTCATGAAGCGCTTTCTGGAACTGTCAACATTCT
GCAAACCTACTTTGAGATGGCAAGAACTGCTGGAGACACACTGGATGTTTTTACCATGATTGACATCTTT
AAGTATGTGATCTACGGCATCGCAGCTGCGTTCTTTGTGTATGGCATTTTGCTGATGGTGGAAGGTTTCT
TCACAACTGGGGCCATCAAAGATCTCTATGGGGATTTCAAAATCACCACTTGTGGCAGATGTGTGAGCGC
TTGGTTCATTATGCTGACATATCTTTTCATGTTGGCCTGGCTGGGAGTCACGGCTTTCACCTCACTGCCA
GTTTACATGTACTTCAATCTGTGGACCATCTGCCGGAACACCACATTAGTGGAGGGAGCAAATCTCTGCT
TGGACCTTCGTCAGTTTGGAATTGTGACAATTGGAGAGGAAAAGAAAATTTGTACTGTCTCTGAGAATTT
CTTGAGGATGTGCGAATCTACTGAGCTGAACATGACCTTCCACTTGTTTATTGTGGCACTTGCTGGAGCT
GGGGCAGCAGTCATTGCTATGGTTCACTACCTTATGGTTCTGTCTGCCAACTGGGCCTATGTGAAAGACG
CCTGCCGGATGCAGAAGTATGAAGACATCAAGTCGAAGGAAGAGCAAGAGCTTCATGACATCCACTCTAC
TCGCTCCAAAGAGCGGCTCAATGCATACACA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC213529 representing NM_201592
Red=Cloning site Green=Tags(s)

MGCFECCIKCLGGIPYASLIATILLYAGVALFCGCGHEALSGTVNILQTYFEMARTAGDTLDVFTMIDIF
KYVIYGIAAAFFVYGILLMVEGFFTTGAIKDLYGDFKITTCGRCVSAWFIMLTYLFMLAWLGVTAFTSLP
VYMYFNLWTICRNTTLVEGANLCLDLRQFGIVTIGEEKKICTVSENFLRMCESTELNMTFHLFIVALAGA
GAAVIAMVHYLMVLSANWAYVKDACRMQKYEDIKSKEEQELHDIHSTRSKERLNAYT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_201592
ORF Size 801 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_201592.2
RefSeq Size 2962 bp
RefSeq ORF 804 bp
Locus ID 2823
UniProt ID P51674
Protein Families Transmembrane
MW 29.7 kDa
Gene Summary Involved in neuronal differentiation, including differentiation and migration of neuronal stem cells. Plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation. GPM6A-induced filopodia formation involves mitogen-activated protein kinase (MAPK) and Src signaling pathways. May be involved in neuronal NGF-dependent Ca(2+) influx. May be involved in regulation of endocytosis and intracellular trafficking of G-protein-coupled receptors (GPCRs); enhances internalization and recycling of mu-type opioid receptor.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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