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MGAT5B (NM_198955) Human Tagged ORF Clone
CAT＃: RC212693
- TrueORF®
MGAT5B (Myc-DDK-tagged)-Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B (MGAT5B), transcript variant 2
ORF Plasmid: tGFP
"NM_198955" in other vectors (4)
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CNY 8,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GnT-IX; GnT-VB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212693 representing NM_198955
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTCATTCGTAAAACATTTGTGCAGCCGGTACGTGGTGGAGCGTCAGGGCACGATGGCCCTTCCTG CCCTCCTGACCCGCCTCCTTCCTCTCCGCAGGCTTTTTGTCCTGGGCATCGGCTTCTTCACTCTCTGCTT CCTGATGACGTCTCTGGGAGGCCAGTTCTCGGCCCGGCGCCTGGGGGACTCGCCATTCACCATCCGCACA GAAGTGATGGGGGGCCCCGAGTCCCGCGGCGTCCTGCGCAAGATGAGCGACCTGCTGGAGCTGATGGTGA AGCGCATGGACGCACTGGCCAGGCTGGAGAACAGCAGTGAGCTGCACCGGGCCGGCGGCGACCTGCACTT TCCCGCAGACAGGATGCCCCCTGGGGCCGGCCTCATGGAGCGGATCCAGGCTATTGCCCAGAACGTCTCC GACATCGCTGTGAAGGTGGACCAGATCCTGCGCCACAGTCTGCTCCTGCACAGCAAGGTGTCAGAAGGCC GGCGGGACCAGTGTGAGGCACCCAGTGACCCCAAGTTCCCTGACTGCTCAGGGAAGGTGGAGTGGATGCG TGCCCGCTGGACCTCTGACCCCTGCTACGCCTTCTTTGGGGTGGACGGCACCGAGTGCTCCTTCCTCATC TACCTCAGTGAGGTCGAGTGGTTCTGCCCCCCGCTGCCCTGGAGGAACCAGACGGCTGCCCAGAGGGCAC CCAAGCCCCTCCCCAAAGTCCAGGCAGTTTTCCGAAGCAACCTGTCCCACCTTCTGGACCTGATGGGCAG CGGGAAGGAGTCCCTGATCTTCATGAAGAAGCGGACCAAGAGGCTCACAGCCCAGTGGGCGCTGGCTGCC CAGCGCCTGGCACAGAAGCTGGGGGCCACCCAGAGGGACCAGAAGCAGATCCTGGTCCACATCGGCTTCC TGACGGAGGAGTCCGGGGACGTGTTCAGCCCTCGGGTCCTGAAGGGCGGGCCCCTAGGGGAGATGGTGCA GTGGGCGGACATTCTGACTGCACTCTATGTCCTGGGCCATGGCCTGCGGGTCACAGTCTCCCTGAAGGAG CTGCAGAGTAACTTAGGGGTACCGCCAGGCCGGGGAAGCTGCCCGCTCACCATGCCCCTGCCCTTCGACC TCATCTACACCGACTACCACGGCCTGCAGCAGATGAAGCGGCACATGGGACTCTCCTTCAAGAAGTACCG GTGCCGAATCAGGGTCATCGACACCTTCGGGACGGAACCTGCGTACAACCACGAGGAGTACGCCACGCTG CACGGCTACCGGACCAACTGGGGCTACTGGAACCTCAACCCCAAGCAGTTCATGACCATGTTTCCTCATA CCCCCGACAACTCCTTCATGGGCTTCGTGTCCGAGGAGCTCAACGAGACGGAGAAGCGGCTCATCAAAGG CGGCAAGGCCAGCAACATGGCCGTGGTGTACGGCAAGGAGGCGAGCATCTGGAAGGGGAAGGAGAAGTTC CTGGGCATCCTGAACAAATACATGGAGATCCATGGCACCGTGTACTACGAGAGCCAGCGGCCCCCCGAGG TGCCAGCCTTTGTGAAGAACCACGGCCTCTTACCGCAGCCTGAGTTTCAGCAGCTGCTGCGCAAGGCCAA ACTCTTCATCGGGTTTGGCTTCCCCTACGAGGGCCCCGCCCCCCTGGAGGCCATCGCCAATGGTTGCATC TTCCTGCAGTCCCGCTTCAGCCCGCCCCACAGCTCCCTCAACCACGAGTTCTTCCGAGGCAAGCCCACCT CCAGAGAGGTGTTCTCCCAGCATCCCTACGCGGAGAACTTCATCGGCAAGCCCCACGTGTGGACAGTCGA CTACAACAACTCAGAGGAGTTTGAAGCAGCCATCAAGGCCATTATGAGAACTCAGGTAGACCCCTACCTA CCCTACGAGTACACCTGCGAGGGGATGCTGGAGCGGATCCACGCCTACATCCAGCACCAGGACTTCTGCA GAGCTCCAGACCCTGCCCTACCAGAGGCCCACGCCCCGCAGAGCCCCTTTGTCCTGGCCCCCAATGCCAC CCACCTCGAGTGGGCTCGGAACACCAGCTTGGCTCCTGGGGCCTGGCCCCCCGCGCACGCCCTGCGGGCC TGGCTGGCCGTGCCTGGGAGGGCCTGCACCGACACCTGCCTGGACCACGGGCTAATCTGTGAGCCCTCCT TCTTCCCCTTCCTGAACAGCCAGGACGCCTTCCTCAAGCTGCAGGTGCCCTGTGACAGCACCGAGTCGGA GATGAACCACCTGTACCCGGCGTTCGCCCAGCCTGGCCAGGAGTGCTACCTGCAGAAGGAGCCTCTGCTC TTCAGCTGCGCCGGCTCCAACACCAAGTACCGCCGGCTCTGCCCCTGCCGCGACTTCCGCAAGGGCCAGG TGGCCTTGTGCCAGGGCTGTCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212693 representing NM_198955
Red=Cloning site Green=Tags(s) MHSFVKHLCSRYVVERQGTMALPALLTRLLPLRRLFVLGIGFFTLCFLMTSLGGQFSARRLGDSPFTIRT EVMGGPESRGVLRKMSDLLELMVKRMDALARLENSSELHRAGGDLHFPADRMPPGAGLMERIQAIAQNVS DIAVKVDQILRHSLLLHSKVSEGRRDQCEAPSDPKFPDCSGKVEWMRARWTSDPCYAFFGVDGTECSFLI YLSEVEWFCPPLPWRNQTAAQRAPKPLPKVQAVFRSNLSHLLDLMGSGKESLIFMKKRTKRLTAQWALAA QRLAQKLGATQRDQKQILVHIGFLTEESGDVFSPRVLKGGPLGEMVQWADILTALYVLGHGLRVTVSLKE LQSNLGVPPGRGSCPLTMPLPFDLIYTDYHGLQQMKRHMGLSFKKYRCRIRVIDTFGTEPAYNHEEYATL HGYRTNWGYWNLNPKQFMTMFPHTPDNSFMGFVSEELNETEKRLIKGGKASNMAVVYGKEASIWKGKEKF LGILNKYMEIHGTVYYESQRPPEVPAFVKNHGLLPQPEFQQLLRKAKLFIGFGFPYEGPAPLEAIANGCI FLQSRFSPPHSSLNHEFFRGKPTSREVFSQHPYAENFIGKPHVWTVDYNNSEEFEAAIKAIMRTQVDPYL PYEYTCEGMLERIHAYIQHQDFCRAPDPALPEAHAPQSPFVLAPNATHLEWARNTSLAPGAWPPAHALRA WLAVPGRACTDTCLDHGLICEPSFFPFLNSQDAFLKLQVPCDSTESEMNHLYPAFAQPGQECYLQKEPLL FSCAGSNTKYRRLCPCRDFRKGQVALCQGCL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198955 |
| ORF Size | 2403 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_198955.1, NP_945193.1 |
| RefSeq Size | 4068 bp |
| RefSeq ORF | 2406 bp |
| Locus ID | 146664 |
| UniProt ID | Q3V5L5 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| MW | 90.4 kDa |
| Gene Summary | The MGAT5B gene encodes a beta-1,6-N-acetylglucosaminyltransferase (EC 2.4.1.155) that functions in the synthesis of complex cell surface N-glycans (Kaneko et al., 2003 [PubMed 14623122]).[supplied by OMIM, Nov 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212693L3 | Lenti-ORF clone of MGAT5B (Myc-DDK-tagged)-Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B (MGAT5B), transcript variant 2 |
CNY 7,700.00 |
|
| RC212693L4 | Lenti-ORF clone of MGAT5B (mGFP-tagged)-Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B (MGAT5B), transcript variant 2 |
CNY 7,700.00 |
|
| RG212693 | MGAT5B (tGFP-tagged) - Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B (MGAT5B), transcript variant 2 |
CNY 6,370.00 |
|
| SC307830 | MGAT5B (untagged)-Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B (MGAT5B), transcript variant 2 |
CNY 15,390.00 |
