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Influenza Virus NS1A Binding Protein (IVNS1ABP) (NM_006469) Human Tagged ORF Clone
CAT＃: RC209701
IVNS1ABP (Myc-DDK-tagged)-Human influenza virus NS1A binding protein (IVNS1ABP)
ORF Plasmid: tGFP
"NM_006469" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARA3; FLARA3; HSPC068; IMD70; KLHL39; ND1; NS-1; NS1-BP; NS1BP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209701 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCCCAATGGATATTTGATGTTTGAGGATGAAAATTTTATTGAGTCTTCTGTTGCCAAATTAAATG CCCTGAGGAAAAGTGGCCAGTTCTGTGATGTTCGACTTCAGGTCTGTGGCCATGAAATGTTAGCACACAG AGCAGTGCTAGCTTGCTGCAGTCCCTATTTATTTGAAATCTTTAATAGTGATAGTGATCCTCATGGAATT TCTCACGTTAAATTTGATGATCTCAATCCAGAAGCTGTTGAAGTCTTGTTGAATTATGCCTACACTGCTC AGTTGAAAGCAGATAAGGAATTGGTAAAAGATGTTTATTCTGCAGCAAAAAAGCTGAAGATGGATCGAGT AAAGCAGGTTTGTGGTGATTATTTACTGTCTAGAATGGATGTTACCAGCTGCATCTCTTACCGAAATTTT GCAAGTTGTATGGGAGACTCCCGTTTGTTGAATAAGGTTGATGCTTATATTCAGGAGCATTTGTTACAAA TTTCTGAAGAGGAGGAGTTTCTTAAGCTTCCAAGGCTAAAGTTGGAGGTAATGCTTGAAGATAATGTTTG CTTGCCCAGCAATGGCAAATTATATACAAAGGTAATCAACTGGGTGCAGCGTAGCATCTGGGAGAATGGA GACAGTCTGGAAGAGCTGATGGGAGAGGTTCAAACCTTGTACTACTCAGCTGATCACAAGCTGCTTGATG GGAACCTACTAGATGGACAGGCTGAGGTGTTTGGCAGTGATGATGACCACATTCAGTTTGTGCAGAAAAA GCCACCACGTGAGAATGGCCATAAGCAGATAAGTAGCAGTTCAACTGGATGTCTCTCTTCTCCAAATGCT ACAGTACAAAGCCCTAAGCATGAGTGGAAAATCGTTGCTTCAGAAAAGACTTCAAATAACACTTACTTGT GCCTGGCTGTGCTGGATGGTATATTCTGTGTCATTTTTCTTCATGGGAGAAACAGCCCACAGAGCTCACC AACAAGTACTCCAAAACTAAGTAAGAGTTTAAGCTTTGAGATGCAACAAGATGAGCTAATCGAAAAGCCC ATGTCTCCTATGCAGTACGCACGATCTGGTCTGGGAACAGCAGAGATGAATGGCAAACTCATAGCTGCAG GTGGCTATAACAGAGAGGAATGTCTTCGAACAGTCGAATGCTATAATCCACATACAGATCACTGGTCCTT TCTTGCTCCCATGAGAACACCAAGAGCCCGATTTCAAATGGCTGTACTCATGGGCCAGCTCTATGTGGTA GGTGGATCAAATGGCCACTCAGATGACCTGAGTTGTGGAGAGATGTATGATTCAAACATAGATGACTGGA TTCCTGTTCCAGAATTGAGAACTAACCGTTGTAATGCAGGAGTGTGTGCTCTGAATGGAAAGTTATACAT CGTTGGTGGCTCTGATCCATATGGTCAAAAAGGACTGAAAAATTGTGATGTATTTGATCCTGTAACAAAG TTGTGGACAAGCTGTGCCCCTCTTAACATTCGGAGACACCAGTCTGCAGTCTGTGAGCTTGGTGGTTATT TGTACATAATCGGAGGTGCAGAATCTTGGAATTGTCTGAACACAGTAGAACGATACAATCCTGAAAATAA TACCTGGACTTTAATTGCACCCATGAATGTGGCTAGGCGAGGAGCTGGAGTGGCTGTTCTTAATGGAAAA CTGTTTGTATGTGGTGGCTTTGATGGTTCTCATGCCATCAGTTGTGTGGAAATGTATGATCCAACTAGAA ATGAATGGAAGATGATGGGAAATATGACTTCACCAAGGAGCAATGCTGGGATTGCAACTGTAGGGAACAC CATTTATGCAGTGGGAGGATTCGATGGCAATGAATTTCTGAATACGGTGGAAGTCTATAACCTTGAGTCA AATGAATGGAGCCCCTATACAAAGATTTTCCAGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209701 protein sequence
Red=Cloning site Green=Tags(s) MIPNGYLMFEDENFIESSVAKLNALRKSGQFCDVRLQVCGHEMLAHRAVLACCSPYLFEIFNSDSDPHGI SHVKFDDLNPEAVEVLLNYAYTAQLKADKELVKDVYSAAKKLKMDRVKQVCGDYLLSRMDVTSCISYRNF ASCMGDSRLLNKVDAYIQEHLLQISEEEEFLKLPRLKLEVMLEDNVCLPSNGKLYTKVINWVQRSIWENG DSLEELMGEVQTLYYSADHKLLDGNLLDGQAEVFGSDDDHIQFVQKKPPRENGHKQISSSSTGCLSSPNA TVQSPKHEWKIVASEKTSNNTYLCLAVLDGIFCVIFLHGRNSPQSSPTSTPKLSKSLSFEMQQDELIEKP MSPMQYARSGLGTAEMNGKLIAAGGYNREECLRTVECYNPHTDHWSFLAPMRTPRARFQMAVLMGQLYVV GGSNGHSDDLSCGEMYDSNIDDWIPVPELRTNRCNAGVCALNGKLYIVGGSDPYGQKGLKNCDVFDPVTK LWTSCAPLNIRRHQSAVCELGGYLYIIGGAESWNCLNTVERYNPENNTWTLIAPMNVARRGAGVAVLNGK LFVCGGFDGSHAISCVEMYDPTRNEWKMMGNMTSPRSNAGIATVGNTIYAVGGFDGNEFLNTVEVYNLES NEWSPYTKIFQF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006469 |
| ORF Size | 1929 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006469.5 |
| RefSeq Size | 4113 bp |
| RefSeq ORF | 1929 bp |
| Locus ID | 10625 |
| UniProt ID | Q9Y6Y0 |
| Domains | BTB, Kelch |
| Protein Families | Transcription Factors |
| MW | 71.7 kDa |
| Gene Summary | Involved in many cell functions, including pre-mRNA splicing, the aryl hydrocarbon receptor (AHR) pathway, F-actin organization and protein ubiquitination. Plays a role in the dynamic organization of the actin skeleton as a stabilizer of actin filaments by association with F-actin through Kelch repeats (By similarity). Protects cells from cell death induced by actin destabilization (By similarity). Functions as modifier of the AHR/Aryl hydrocarbon receptor pathway increasing the concentration of AHR available to activate transcription (PubMed:16582008). In addition, functions as a negative regulator of BCR(KLHL20) E3 ubiquitin ligase complex to prevent ubiquitin-mediated proteolysis of PML and DAPK1, two tumor suppressors (PubMed:25619834). Inhibits pre-mRNA splicing (in vitro) (PubMed:9696811).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209701L3 | Lenti ORF clone of Human influenza virus NS1A binding protein (IVNS1ABP), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209701L4 | Lenti ORF clone of Human influenza virus NS1A binding protein (IVNS1ABP), mGFP tagged |
CNY 5,890.00 |
|
| RG209701 | IVNS1ABP (tGFP-tagged) - Human influenza virus NS1A binding protein (IVNS1ABP) |
CNY 4,370.00 |
|
| SC315818 | IVNS1ABP (untagged)-Human influenza virus NS1A binding protein (IVNS1ABP) |
CNY 7,220.00 |
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