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F8A2 (NM_001007523) Human Tagged ORF Clone
CAT＃: RC207473
F8A2 (Myc-DDK-tagged)-Human coagulation factor VIII-associated 2 (F8A2)
ORF Plasmid: tGFP
"NM_001007523" in other vectors (4)
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CNY 3,656.00
CNY 4,370.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HAP40 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207473 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCAGCGGCTGCAGGCCTGGGCGGCGGCGGCGCCGGCCCGGGACCCGAGGCCGGGGACTTCCTGG CCCGCTACCGGCTGGTATCGAACAAGCTGAAGAAGCGGTTCCTGCGGAAGCCGAACGTGGCGGAGGCCGG CGAGCAGTTCGGACAGCTGGGCCGGGAGCTGCGCGCCCAGGAGTGTCTGCCCTACGCGGCCTGGTGCCAG CTGGCGGTGGCGCGCTGCCAGCAGGCGCTCTTCCACGGGCCCGGGGAGGCGCTGGCCCTCACCGAGGCCG CCCGCCTCTTCCTGCGGCAGGAGCGCGACGCGCGCCAGCGCCTGGTCTGCCCCGCCGCCTACGGGGAGCC GCTGCAGGCCGCCGCCAGCGCCCTGGGCGCCGCGGTGCGTCTGCACCTCGAGCTGGGCCAGCCGGCCGCC GCCGCCGCCCTCTGCCTCGAGCTGGCCGCCGCCCTGCGCGACCTGGGCCAGCCGGCCGCCGCCGCCGGTC ACTTCCAGCGCGCCGCCCAGCTCCAGCTGCCCCAGCTGCCCCTGGCCGCGCTGCAGGCGCTTGGCGAGGC CGCCTCCTGCCAGCTGCTGGCGCGCGACTACACCGGCGCCCTGGCGGTCTTCACGCGCATGCAGCGCCTG GCGCGGGAGCACGGCAGCCACCCGGTGCAGTCACTGCCGCCGCCCCCGCCGCCGGCACCCCAGCCCGGGC CCGGGGCGACGCCCGCCCTACCGGCCGCGCTGCTTCCTCCGAACTCCGGCTCGGCGGCGCCCTCTCCCGC CGCCCTGGGCGCCTTCTCGGACGTGCTGGTCCGCTGCGAGGTGTCCCGCGTGCTGCTGCTGCTCCTCCTG CAACCACCGCCCGCCAAGCTGCTGCCGGAGCACGCCCAGACCCTGGAGAAGTACTCCTGGGAGGCTTTTG ACAGCCACGGGCAGGAGAGCAGCGGCCAGCTTCCCGAGGAGCTCTTTCTGCTGCTCCAGTCTTTGGTCAT GGCTACCCACGAAAAGGACACGGAAGCCATCAAGTCGCTGCAGGTGGAGATGTGGCCACTGTTGACTGCT GAGCAGAACCACCTCCTTCACCTCGTTCTGCAAGAAACCATCTCCCCCTCAGGACAGGGAGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207473 protein sequence
Red=Cloning site Green=Tags(s) MAAAAAGLGGGGAGPGPEAGDFLARYRLVSNKLKKRFLRKPNVAEAGEQFGQLGRELRAQECLPYAAWCQ LAVARCQQALFHGPGEALALTEAARLFLRQERDARQRLVCPAAYGEPLQAAASALGAAVRLHLELGQPAA AAALCLELAAALRDLGQPAAAAGHFQRAAQLQLPQLPLAALQALGEAASCQLLARDYTGALAVFTRMQRL AREHGSHPVQSLPPPPPPAPQPGPGATPALPAALLPPNSGSAAPSPAALGAFSDVLVRCEVSRVLLLLLL QPPPAKLLPEHAQTLEKYSWEAFDSHGQESSGQLPEELFLLLQSLVMATHEKDTEAIKSLQVEMWPLLTA EQNHLLHLVLQETISPSGQGV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001007523 |
| ORF Size | 1113 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001007523.1, NP_001007524.1 |
| RefSeq Size | 1116 bp |
| RefSeq ORF | 1116 bp |
| Locus ID | 474383 |
| UniProt ID | P23610 |
| MW | 39.1 kDa |
| Gene Summary | This gene is part of a region that is repeated three times on chromosome X, once in intron 22 of the F8 gene and twice closer to the Xq telomere. This record represents the middle copy. Although its function is unknown, the observation that this gene is conserved in the mouse implies it has some function. Unlike factor VIII, this gene is transcribed abundantly in a wide variety of cell types. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207473L3 | Lenti ORF clone of Human coagulation factor VIII-associated 2 (F8A2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207473L4 | Lenti ORF clone of Human coagulation factor VIII-associated 2 (F8A2), mGFP tagged |
CNY 5,890.00 |
|
| RG207473 | F8A2 (tGFP-tagged) - Human coagulation factor VIII-associated 2 (F8A2) |
CNY 4,370.00 |
|
| SC301219 | F8A2 (untagged)-Human coagulation factor VIII-associated 2 (F8A2) |
CNY 6,080.00 |
