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Estrogen Sulfotransferase (SULT1E1) (NM_005420) Human Tagged ORF Clone
CAT＃: RC206535
SULT1E1 (Myc-DDK-tagged)-Human sulfotransferase family 1E, estrogen-preferring, member 1 (SULT1E1)
ORF Plasmid: tGFP
"NM_005420" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EST; EST-1; ST1E1; STE |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206535 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTCTGAACTTGACTATTATGAAAAGTTTGAAGAAGTCCATGGGATTCTAATGTATAAAGATTTTG TCAAATATTGGGATAATGTGGAAGCGTTCCAGGCAAGACCAGATGATCTTGTCATTGCCACCTACCCTAA ATCTGGTACAACCTGGGTTAGTGAAATTGTGTATATGATCTATAAAGAGGGTGATGTGGAAAAGTGCAAA GAAGATGTAATTTTTAATCGAATACCTTTCCTGGAATGCAGAAAAGAAAACCTCATGAATGGAGTAAAAC AATTAGATGAGATGAATTCTCCTAGAATTGTGAAGACTCATTTGCCACCTGAACTTCTTCCTGCCTCATT TTGGGAAAAGGATTGTAAGATAATCTATCTTTGCCGGAATGCAAAGGATGTGGCTGTTTCCTTTTATTAT TTCTTTCTAATGGTGGCTGGTCATCCAAATCCTGGATCCCTTCCAGAGTTTGTGGAGAAATTCATGCAAG GACAGGTTCCTTATGGTTCCTGGTATAAACATGTAAAATCTTGGTGGGAAAAGGGAAAGAGTCCACGTGT ACTATTTCTTTTCTACGAAGACCTGAAAGAGGATATCAGAAAAGAGGTGATAAAATTGATACATTTCCTG GAAAGGAAGCCATCAGAGGAGCTTGTGGACAGGATTATACATCATACTTCGTTCCAAGAGATGAAGAACA ATCCATCCACAAATTACACAACACTGCCAGACGAAATTATGAACCAGAAATTGTCGCCCTTCATGAGAAA GGGAATTACAGGAGACTGGAAAAATCACTTTACAGTAGCCCTGAATGAAAAATTTGATAAACATTATGAG CAGCAAATGAAGGAATCTACACTGAAGTTTCGAACTGAGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206535 protein sequence
Red=Cloning site Green=Tags(s) MNSELDYYEKFEEVHGILMYKDFVKYWDNVEAFQARPDDLVIATYPKSGTTWVSEIVYMIYKEGDVEKCK EDVIFNRIPFLECRKENLMNGVKQLDEMNSPRIVKTHLPPELLPASFWEKDCKIIYLCRNAKDVAVSFYY FFLMVAGHPNPGSLPEFVEKFMQGQVPYGSWYKHVKSWWEKGKSPRVLFLFYEDLKEDIRKEVIKLIHFL ERKPSEELVDRIIHHTSFQEMKNNPSTNYTTLPDEIMNQKLSPFMRKGITGDWKNHFTVALNEKFDKHYE QQMKESTLKFRTEI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005420 |
| ORF Size | 882 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005420.3 |
| RefSeq Size | 1805 bp |
| RefSeq ORF | 885 bp |
| Locus ID | 6783 |
| UniProt ID | P49888 |
| Protein Pathways | Androgen and estrogen metabolism, Sulfur metabolism |
| MW | 35.1 kDa |
| Gene Summary | Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a protein that transfers a sulfo moiety to and from estrone, which may control levels of estrogen receptors. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Overexpression of steroid sulfotransferase genes is associated with worsened prognosis and with immune exclusion in clear cell-renal cell carcinoma
,Li, Y;Ding, Q;Xiong, Z;Wen, H;Feng, C;,
Aging (Albany NY) 2019
,PubMed ID 31655797
[Estrogen Sulfotransferase]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206535L3 | Lenti ORF clone of Human sulfotransferase family 1E, estrogen-preferring, member 1 (SULT1E1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206535L4 | Lenti ORF clone of Human sulfotransferase family 1E, estrogen-preferring, member 1 (SULT1E1), mGFP tagged |
CNY 5,890.00 |
|
| RG206535 | SULT1E1 (tGFP-tagged) - Human sulfotransferase family 1E, estrogen-preferring, member 1 (SULT1E1) |
CNY 4,000.00 |
|
| SC122715 | SULT1E1 (untagged)-Human sulfotransferase family 1E, estrogen-preferring, member 1 (SULT1E1) |
CNY 2,400.00 |
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