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ACCN4 (ASIC4) (NM_182847) Human Tagged ORF Clone
CAT＃: RC203827
ASIC4 (Myc-DDK-tagged)-Human amiloride-sensitive cation channel 4, pituitary (ACCN4), transcript variant 2
ORF Plasmid: tGFP
"NM_182847" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ACCN4; BNAC4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203827 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAGCGGAGCGGCTGGGGCTGCGCGGCGTGGCGGAGCAGCGCTCGCTCCCTCGCTCACTCGCTCGC TCGCAGGGACACACGCAGGGGCTGACAGCTGTGCTGGTGCTGATAAGGGAAGCCACAAGGAGACGATCGA GGAGAGAGACAAGCGGCAGCAGAGGCAGCAGCGGCAGAGGCAGCACCAGGGCTGCGGAGCTGCTGGGAGT GGGAGTGACTCCCCCACCTCGGGCCCCCACCCTGTCCCTGTCCTCTTCCCGCTTGCCCTGAGTTTAGAAG AGCAGCCGCTGCCACCACTGCCACTCGGGAGGGCACCAGGGCTGCTGGCTAGGGAGGGACAGGGCAGGGA GGCTCTGGCCAGTCCCAGCAGCCGGGGACAGATGCCGATCGAGATTGTGTGCAAAATCAAATTTGCTGAG GAGGATGCGAAACCCAAGGAGAAGGAGGCAGGGGATGAGCAGAGCCTCCTCGGGGCTGTTGCCCCTGGAG CAGCCCCCCGAGACCTGGCCACCTTTGCCAGCACCAGCACCCTGCATGGACTGGGCCGGGCCTGTGGCCC AGGCCCCCACGGACTGCGCAGAACCCTGTGGGCACTGGCCCTACTCACCTCGCTGGCTGCCTTCCTGTAC CAGGCGGCTGGCCTGGCCCGGGGCTACCTGACCCGGCCTCACCTGGTGGCAATGGACCCCGCTGCCCCAG CCCCAGTGGCGGGCTTCCCGGCTGTCACCCTCTGCAATATCAACCGCTTCCGGCATTCGGCACTCAGCGA TGCCGACATCTTCCACCTGGCCAATCTGACAGGGCTGCCCCCCAAAGACCGGGATGGGCACCGTGCGGCT GGCCTGCGCTACCCAGAGCCTGACATGGTAGACATCCTCAACCGCACTGGCCACCAGCTCGCCGACATGC TTAAGAGCTGCAACTTCAGTGGGCATCACTGCTCCGCCAGCAACTTCTCTGTGGTCTATACTCGCTATGG GAAGTGTTACACCTTCAACGCGGACCCGCGGAGCTCGCTGCCCAGCCGGGCAGGGGGCATGGGCAGTGGC CTGGAGATCATGCTGGACATCCAGCAGGAGGAGTACCTGCCCATCTGGAGGGAGACAAATGAGACGTCGT TTGAGGCAGGTATTCGGGTGCAGATCCACAGCCAGGAGGAGCCGCCCTACATCCACCAGCTGGGGTTCGG GGTGTCCCCAGGCTTCCAGACCTTTGTGTCCTGCCAGGAACAGCGGCTGACCTACCTGCCCCAGCCCTGG GGCAACTGCCGCGCAGAGAGTGAGCTCAGGGAGCCTGAGCTTCAGGGCTACTCGGCCTACAGTGTGTCTG CCTGCCGGCTGCGCTGTGAAAAGGAGGCCGTGCTTCAGCGCTGCCACTGCCGGATGGTGCACATGCCAGA CTCCCTGGGTGGGGGCCCTGAGGGCCCGTGCTTCTGCCCCACCCCCTGCAACCTGACACGCTATGGGAAA GAGATCTCCATGGTCAGGATCCCCAACAGGGGCTCAGCCCGGTACCTGGCGAGGAAGTACAACCGCAACG AGACCTACATACGGGAGAACTTCCTGGTCCTAGATGTCTTCTTTGAGGCCCTGACCTCTGAAGCCATGGA GCAGCGAGCAGCCTATGGCCTGTCAGCCCTGCTGGGAGACCTCGGGGGACAGATGGGCCTGTTCATTGGG GCCAGCATCCTCACGTTGCTGGAGATCCTCGACTACATCTATGAGGTGTCCTGGGATCGACTGAAGCGGG TATGGAGGCGTCCCAAGACCCCCCTGCGGACCTCCACTGGGGGCATCTCCACTTTGGGGCTTCAGGAGCT GAAGGAACAGAGTCCCTGCCCGAGCCTGGGCCGAGCGGAGGGTGGGGGGGTCAGCAGTCTGCTCCCCAAT CACCACCACCCCCACGGTCCCCCAGGAGGTCTCTTTGAAGATTTTGCTTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203827 protein sequence
Red=Cloning site Green=Tags(s) MLSGAAGAARRGGAALAPSLTRSLAGTHAGADSCAGADKGSHKETIEERDKRQQRQQRQRQHQGCGAAGS GSDSPTSGPHPVPVLFPLALSLEEQPLPPLPLGRAPGLLAREGQGREALASPSSRGQMPIEIVCKIKFAE EDAKPKEKEAGDEQSLLGAVAPGAAPRDLATFASTSTLHGLGRACGPGPHGLRRTLWALALLTSLAAFLY QAAGLARGYLTRPHLVAMDPAAPAPVAGFPAVTLCNINRFRHSALSDADIFHLANLTGLPPKDRDGHRAA GLRYPEPDMVDILNRTGHQLADMLKSCNFSGHHCSASNFSVVYTRYGKCYTFNADPRSSLPSRAGGMGSG LEIMLDIQQEEYLPIWRETNETSFEAGIRVQIHSQEEPPYIHQLGFGVSPGFQTFVSCQEQRLTYLPQPW GNCRAESELREPELQGYSAYSVSACRLRCEKEAVLQRCHCRMVHMPDSLGGGPEGPCFCPTPCNLTRYGK EISMVRIPNRGSARYLARKYNRNETYIRENFLVLDVFFEALTSEAMEQRAAYGLSALLGDLGGQMGLFIG ASILTLLEILDYIYEVSWDRLKRVWRRPKTPLRTSTGGISTLGLQELKEQSPCPSLGRAEGGGVSSLLPN HHHPHGPPGGLFEDFAC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_182847 |
| ORF Size | 1941 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_182847.1, NP_878267.1 |
| RefSeq Size | 2857 bp |
| RefSeq ORF | 1563 bp |
| Locus ID | 55515 |
| UniProt ID | Q96FT7 |
| Protein Families | Druggable Genome, Ion Channels: Other |
| MW | 70 kDa |
| Gene Summary | This gene belongs to the superfamily of acid-sensing ion channels, which are proton-gated, amiloride-sensitive sodium channels. These channels have been implicated in synaptic transmission, pain perception as well as mechanoperception. This gene is predominantly expressed in the pituitary gland, and was considered a candidate for paroxysmal dystonic choreoathetosis (PDC), a movement disorder, however, no correlation was found between mutations in this gene and PDC. [provided by RefSeq, Feb 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203827L3 | Lenti ORF clone of Human amiloride-sensitive cation channel 4, pituitary (ACCN4), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203827L4 | Lenti ORF clone of Human amiloride-sensitive cation channel 4, pituitary (ACCN4), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG203827 | ASIC4 (tGFP-tagged) - Human amiloride-sensitive cation channel 4, pituitary (ACCN4), transcript variant 2 |
CNY 4,370.00 |
|
| SC324144 | ASIC4 (untagged)-Human amiloride-sensitive cation channel 4, pituitary (ACCN4), transcript variant 2 |
CNY 4,824.00 |
