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PAAF1 (NM_025155) Human Tagged ORF Clone
CAT＃: RC202994
PAAF1 (Myc-DDK-tagged)-Human proteasomal ATPase-associated factor 1 (PAAF1)
ORF Plasmid: tGFP
"NM_025155" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PAAF; Rpn14; WDR71 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC202994 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCCTTTGAGGATTCAGAGCGACTGGGCGCAAGCCCTCAGGAAGGATGAAGGGGAGGCCTGGC TGAGCTGTCATCCCCCAGGGAAACCATCTTTGTATGGCAGCCTGACTTGTCAAGGAATTGGCCTAGATGG CATCCCAGAGGTTACAGCTTCAGAAGGATTTACTGTGAATGAAATAAACAAGAAAAGCATTCATATTTCA TGTCCAAAGGAAAATGCATCTTCTAAGTTTTTGGCACCATATACTACTTTTTCCAGAATTCATACAAAGA GTATAACATGCCTGGACATTTCCAGCAGAGGAGGTCTTGGTGTGTCTTCTAGTACTGACGGGACCATGAA AATCTGGCAGGCTTCCAATGGAGAACTCAGGAGAGTATTGGAAGGACATGTGTTTGATGTGAATTGTTGC AGGTTTTTCCCATCAGGCCTTGTGGTCCTGAGTGGGGGAATGGATGCCCAGCTGAAGATATGGTCAGCTG AAGATGCTAGCTGCGTGGTGACCTTCAAAGGTCACAAAGGAGGTATCCTGGATACAGCCATCGTTGATCG GGGGAGGAATGTGGTGTCTGCTTCTCGAGATGGGACAGCACGACTTTGGGATTGTGGGCGCTCAGGCTGC TTGGGAGTCCTTGCAGATTGTGGTTCTTCTATCAATGGAGTGGCGGTGGGTGCTGCTGACAACTCCATAA ACCTTGGCTCCCCTGAGCAGATGCCCAGTGAACGGGAGGTTGGAACAGAGGCCAAAATGCTGCTCTTGGC CCGGGAAGATAAGAAACTTCAGTGCTTGGGACTACAGAGCAGGCAGCTGGTGTTCCTCTTTATTGGCTCA GACGCTTTCAACTGCTGTACTTTTCTCTCTGGCTTCTTGCTATTGGCTGGGACTCAAGATGGAAACATTT ATCAGCTGGATGTGAGGAGTCCAAGGGCTCCGGTACAAGTCATCCACAGATCAGGAGCACCAGTTCTATC CCTGCTAAGTGTCAGAGATGGATTCATTGCTAGCCAAGGTGATGGAAGCTGTTTTATTGTCCAGCAAGAC TTAGACTATGTCACTGAGCTCACTGGGGCTGACTGTGACCCTGTGTACAAGGTAGCCACATGGGAGAAGC AGATCTACACATGCTGTCGAGACGGTCTTGTACGACGCTACCAGCTTTCTGACCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202994 protein sequence
Red=Cloning site Green=Tags(s) MAAPLRIQSDWAQALRKDEGEAWLSCHPPGKPSLYGSLTCQGIGLDGIPEVTASEGFTVNEINKKSIHIS CPKENASSKFLAPYTTFSRIHTKSITCLDISSRGGLGVSSSTDGTMKIWQASNGELRRVLEGHVFDVNCC RFFPSGLVVLSGGMDAQLKIWSAEDASCVVTFKGHKGGILDTAIVDRGRNVVSASRDGTARLWDCGRSGC LGVLADCGSSINGVAVGAADNSINLGSPEQMPSEREVGTEAKMLLLAREDKKLQCLGLQSRQLVFLFIGS DAFNCCTFLSGFLLLAGTQDGNIYQLDVRSPRAPVQVIHRSGAPVLSLLSVRDGFIASQGDGSCFIVQQD LDYVTELTGADCDPVYKVATWEKQIYTCCRDGLVRRYQLSDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025155 |
| ORF Size | 1176 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025155.3 |
| RefSeq Size | 1626 bp |
| RefSeq ORF | 1179 bp |
| Locus ID | 80227 |
| UniProt ID | Q9BRP4 |
| Domains | WD40 |
| MW | 42.2 kDa |
| Gene Summary | This gene encodes a WD repeat-containing protein involved in regulation of association of proteasome components. During HIV infection, the encoded protein is thought to promote provirus transcription through recruitment of the 19S regulatory complex. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jun 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202994L1 | Lenti ORF clone of Human proteasomal ATPase-associated factor 1 (PAAF1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202994L2 | Lenti ORF clone of Human proteasomal ATPase-associated factor 1 (PAAF1), mGFP tagged |
CNY 5,890.00 |
|
| RC202994L3 | Lenti ORF clone of Human proteasomal ATPase-associated factor 1 (PAAF1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202994L4 | Lenti ORF clone of Human proteasomal ATPase-associated factor 1 (PAAF1), mGFP tagged |
CNY 5,890.00 |
|
| RG202994 | PAAF1 (tGFP-tagged) - Human proteasomal ATPase-associated factor 1 (PAAF1) |
CNY 4,370.00 |
|
| SC110591 | PAAF1 (untagged)-Human proteasomal ATPase-associated factor 1 (PAAF1) |
CNY 7,220.00 |
