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SGT1 (ECD) (NM_007265) Human Tagged ORF Clone
CAT＃: RC200384
ECD (Myc-DDK-tagged)-Human ecdysoneless homolog (Drosophila) (ECD), transcript variant 1
ORF Plasmid: tGFP
"NM_007265" in other vectors (4)
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CNY 4,792.00
CNY 5,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GCR2; HSGT1; SGT1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC200384 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAAACCATGAAGCTTGCTACGATGGAAGACACAGTGGAGTACTGCCTGTTCCTGATACCAGATG AGTCAAGGGACTCAGATAAACATAAAGAGATTCTTCAGAAGTACATTGAGAGAATAATCACTCGGTTTGC ACCTATGCTGGTCCCCTACATCTGGCAGAATCAGCCTTTCAATCTTAAATATAAACCTGGGAAAGGAGGT GTTCCTGCTCATATGTTTGGCGTGACAAAGTTTGGGGATAACATTGAGGATGAATGGTTTATTGTTTATG TAATAAAGCAGATCACAAAGGAATTTCCAGAGTTAGTAGCAAGGATTGAAGACAATGATGGTGAATTCTT GTTAATAGAAGCTGCTGACTTTCTCCCTAAATGGCTGGATCCTGAAAATAGCACCAATAGGGTATTTTTC TGCCATGGGGAATTGTGTATTATCCCTGCACCAAGAAAATCTGGAGCAGAATCTTGGTTACCCACCACAC CCCCAACAATTCCACAAGCATTGAATATAATCACAGCACATTCAGAAAAAATACTTGCTTCAGAATCTAT ACGAGCTGCTGTGAATAGGCGCATCAGAGGGTACCCAGAAAAAATTCAGGCCTCACTTCATCGAGCACAC TGCTTCCTTCCAGCTGGCATTGTGGCAGTGCTAAAGCAGCGCCCCAGATTGGTGGCTGCAGCAGTCCAGG CATTTTACCTACGAGACCCTATTGACCTGCGAGCTTGTCGTGTTTTCAAGACATTCTTGCCTGAAACACG AATAATGACATCGGTCACATTCACTAAATGTCTATATGCACAATTGGTGCAACAAAGGTTTGTGCCAGAC CGGCGGAGTGGATACAGGCTGCCTCCTCCATCTGATCCCCAGTACCGAGCCCATGAATTGGGCATGAAAT TGGCTCATGGATTTGAGATCTTATGCTCCAAATGTAGCCCACATTTTTCTGACTGCAAGAAATCCCTTGT GACTGCCTCACCACTCTGGGCCAGTTTCCTTGAAAGTCTGAAAAAGAATGATTACTTTAAGGGACTGATA GAAGGTTCTGCTCAGTACCGGGAAAGGCTAGAAATGGCAGAGAATTACTTCCAGCTCTCAGTAGACTGGC CAGAAAGTTCTCTTGCTATGAGCCCTGGTGAAGAAATCTTAACCTTATTACAGACAATACCATTTGATAT AGAAGACCTTAAGAAAGAAGCAGCTAATCTTCCCCCAGAGGATGATGACCAGTGGTTAGATCTCTCACCA GATCAGCTGGACCAGCTGCTGCAGGAAGCTGTTGGCAAAAAAGAATCCGAGTCTGTTTCCAAGGAGGAGA AGGAGCAGAACTATGACTTAACTGAAGTCTCAGAGAGCATGAAAGCTTTCATATCCAAAGTCTCAACCCA CAAGGGAGCAGAGCTGCCTCGAGAACCTTCTGAGGCTCCAATCACTTTTGATGCAGATTCTTTTCTTAAT TATTTTGATAAGATTTTAGGGCCAAGGCCTAATGAGTCAGATTCTGATGATCTGGATGATGAAGACTTTG AATGTTTAGATAGTGATGATGACTTGGACTTTGAAACACACGAACCTGGCGAAGAGGCTTCCCTGAAAGG AACACTTGATAATCTCAAGTCATACATGGCCCAGATGGACCAGGAACTAGCACACACCTGCATCAGCAAA AGTTTCACCACTAGGAACCAAGTGGAACCTGTATCCCAGACTACCGATAACAATTCAGATGAGGAAGATT CTGGTACGGGAGAATCTGTTATGGCACCAGTAGATGTAGACCTGAACCTGGTTTCAAATATATTGGAATC CTATAGCTCCCAAGCTGGACTGGCAGGACCTGCTTCCAATCTTTTACAAAGCATGGGAGTGCAGCTGCCT GACAACACCGATCACAGACCAACAAGTAAGCCAACAAAAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC200384 protein sequence
Red=Cloning site Green=Tags(s) MEETMKLATMEDTVEYCLFLIPDESRDSDKHKEILQKYIERIITRFAPMLVPYIWQNQPFNLKYKPGKGG VPAHMFGVTKFGDNIEDEWFIVYVIKQITKEFPELVARIEDNDGEFLLIEAADFLPKWLDPENSTNRVFF CHGELCIIPAPRKSGAESWLPTTPPTIPQALNIITAHSEKILASESIRAAVNRRIRGYPEKIQASLHRAH CFLPAGIVAVLKQRPRLVAAAVQAFYLRDPIDLRACRVFKTFLPETRIMTSVTFTKCLYAQLVQQRFVPD RRSGYRLPPPSDPQYRAHELGMKLAHGFEILCSKCSPHFSDCKKSLVTASPLWASFLESLKKNDYFKGLI EGSAQYRERLEMAENYFQLSVDWPESSLAMSPGEEILTLLQTIPFDIEDLKKEAANLPPEDDDQWLDLSP DQLDQLLQEAVGKKESESVSKEEKEQNYDLTEVSESMKAFISKVSTHKGAELPREPSEAPITFDADSFLN YFDKILGPRPNESDSDDLDDEDFECLDSDDDLDFETHEPGEEASLKGTLDNLKSYMAQMDQELAHTCISK SFTTRNQVEPVSQTTDNNSDEEDSGTGESVMAPVDVDLNLVSNILESYSSQAGLAGPASNLLQSMGVQLP DNTDHRPTSKPTKN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007265 |
| ORF Size | 1932 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007265.3 |
| RefSeq Size | 2280 bp |
| RefSeq ORF | 1935 bp |
| Locus ID | 11319 |
| UniProt ID | O95905 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 72.8 kDa |
| Gene Summary | Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed:16849563, PubMed:23880345). May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed:19919181, PubMed:9928932). Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed:26711270). May play a role in regulation of pre-mRNA splicing (PubMed:24722212).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200384L3 | Lenti ORF clone of Human ecdysoneless homolog (Drosophila) (ECD), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200384L4 | Lenti ORF clone of Human ecdysoneless homolog (Drosophila) (ECD), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG200384 | ECD (tGFP-tagged) - Human ecdysoneless homolog (Drosophila) (ECD), transcript variant 1 |
CNY 4,370.00 |
|
| SC321385 | ECD (untagged)-Human ecdysoneless homolog (Drosophila) (ECD), transcript variant 1 |
CNY 2,950.00 |
