Sytl2 (NM_001289583) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI266830; mKIAA1597; Slp2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231415 representing NM_001289583 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCGACTTAAGTTTCCTGACAGAGGAGGAGCAAGACGCCATCTTAAAGGTTCTGCAGAGGGATGCTG CCCTGAAGAGGGCTGAAGAGGAGAGAGTCAGACATCTGCCTGAAAAAATTAAGGATGACCAACAACTAAA GAATATGAGTGGCCAATGGTTTTATGAAGCTAAGGCAAAAAGACACAGGGACAAAATCCACGGTGCTGAC ATCATCAGAGCATCCATGAGAAGGAAGAAGCTCCCAGCGGCAGCTGAGCAGAATAAGGACACAGCAATGA GGGCAAAGGAGAGCTGGGTGAATAATGTTAACAAAGATGCTGTCCTTCCTCCAGAGATAGCTGTTGTGGA AGAGCCAGAAGATGACACAGATCCCGCAGGCCCAAGTTCCAGTTTGGTGGATCCAGCTTCCAGTGTGATT GATATGTCCCAGGAAAGTACGAGGACTCCAGCTGTGTCTCTGCCCAAGCAAAGGAAGAATCCATTCAACA GCCCCAAGCTGCCGGAAGATCACTCCTTGCAACAAACCAAACCCGAGCAGTCAAAAACTGGAAAAGCTGG TTTATTTCAGATTTCAAAAGAGGGTGAGTTGTCAGAATCAAAAGAAAAGTCATCTATCCCAGATATGCCA CGTCAGCAGTTAGAGAAACCAAAGCAAACTGTATCCACAGAGCCTGAAAATGCGTCTCATACCAAAGCTC CAATCCCCAAAGCCAGAAAACTGATCTACAAATCAAATGACTTAGAGAAAGATGATAACCAGTCTTTTCC CAGACAACGGAGGGACTCCCTGAATGCGAGAGGGGCTCCAAGAGGGATCCTAAAGCGCAACTCCAGTTCC AGCAGCACGGACTCAGAGACCCTCCGTTTAAATTACAACCTTGATCCGAAAAGCAAAATCCTATCACCTG GCCTAACCATCCACGAGAGAATCTCTGAGAAGGAATTTTCTTTAGAAGACGACTCTTCCACAAGCTCACT AGAGCCGTTAAAGCACGTGAGATTCTCTGCCGTGAAGAATGAGCTTCCACAAAGTCCTAGACCCGTCCTT GGCCAGGAAGTGGGAGAATTTACTGTTTTAGAATCTGACCAGTTGCAAAATGGAACTGAAGATGCAGGGG ACATAGAGGAGTTTCAGAATCACCCAGAGCTCTCCCACAAAACACCTTTGTCTCATTATCAGTTAGTGTC AAGCCCAAGCGATTCAGGAAGGGAAAGAGAACAGCTGATGTCTTCTGGGTCTGCTCCAAGAGATGAGATC CCTTGTCATTCAGACATTCTACCCACAGGACCTCAGTGTGTTGAGAGTTCATCCGTCATCAATGGGCAAC AAGAGAAATCATCACATTTTACAAAGCTTCCATCAGAATTGTCCAAAAGCCCTTCCGATGAACTGACTCA GTGTGGTGAGCCTGAGCCATCACAGACAGCAGACCACAGTTTTAGAGACCATCGGCAAGGTTCAGAAGAA GAGCACAGCCCTGTTTTGAAAACTCTGGAAAGAAGAGCTGCTAGGAAATTGCCTTCCAAAAGTCTAGAAG ACATTCCATCAGATTCATCAAATCAAGCAAAAGTAGATAATCTGCCTGAAGAATTAGTGCGTAGTGCTGA AGATGATCAAAAAGCCGACCAAGAACCAGATACAAATGAATGTATACCAGGAATTTCCACAGTGCCTTCA CTGCCTGATAATCAGTTTTCCCACCCTGACAAACTCAAAAGGATGAGCAAGTCCGTGCCAGCATTTCTTC AAGATGAGAGCGATGACAGAGAAACAGACACAGCATCAGAGAGCAGCTACCAGCTCAGGAGATACAAGAA GAGCCCCAGCTCATTAACCAATCTTAGCAGCTCCTCTGGCATGACGTCCTTGTCCTCTGCGAGTGGCAGT GTGATGAGCGTTTACAGTGGAGACTTTGGCAACCTAGAAGTGAAAGGAAGCGTGCAGTTTGCACTCGACT ACGTGGAGTCCCTGAAAGAGCTGCATGTGTTTGTGGCCCAGTGTAAGGATTTAGCAGCAGCAGATGTTAA GAAACAGCGCTCAGATCCGTATGTAAAGACCTATCTGCTACCAGACAAAGGCAAAATGGGCAAGAAGAAG ACACTCGTAGTGAAGAAGACCTTGAATCCTGTATACAACGAGATATTGCGGTATAAAATTGAAAGGCAAT TCTTAAAGACGCAGAAGTTGAACCTGTCCGTTTGGCATCGGGATACATTTAAGCGCAACAGCTTTCTGGG GGAGGTGGAGCTCGACCTGGAAACGTGGGATTGGGACAGCAAACAGAACAAACAGCTGAAGTGGTACCCA CTGAAGAGGAAGACAGCACCAGTTGCCCTCGAGACAGAAAACAGAGGTGAAATGAAACTAGCTCTCCAGT ATGTTCCGGAACCAAGCCCTGGCAAAAAGCTTCCTACAACTGGAGAAGTCCACATCTGGGTGAAGGAATG CCTTGACCTCCCACTGTTGAGGGGCAGCCACCTAAATTCTTTTGTTAAATGTACCATCCTTCCAGATACC AGTAGAAAAAGTCGCCAGAAGACAAGAGCTGTAGGGAAAACCACCAACCCCGTCTTCAACCATACCATGG TGTATGATGGGTTCAGGCCTGAAGATCTGATGGAAGCCTGTGTAGAACTCACAGTCTGGGACCATTATAA ACTAACCAACCAGTTTCTGGGAGGTCTCCGGATCGGCTTTGGAACAGGAAAAAGCTACGGGACTGAAGTG GATTGGATGGATTCTACTTCTGAGGAAGTTGCTCTCTGGGAGAAGATGGTAAACTCTCCCAACACTTGGG TTGAAGCGACGCTGCCCCTCCGGATGCTTCTGATTGCCAAGCTTTCCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231415 representing NM_001289583 Red=Cloning site Green=Tags(s) MIDLSFLTEEEQDAILKVLQRDAALKRAEEERVRHLPEKIKDDQQLKNMSGQWFYEAKAKRHRDKIHGAD IIRASMRRKKLPAAAEQNKDTAMRAKESWVNNVNKDAVLPPEIAVVEEPEDDTDPAGPSSSLVDPASSVI DMSQESTRTPAVSLPKQRKNPFNSPKLPEDHSLQQTKPEQSKTGKAGLFQISKEGELSESKEKSSIPDMP RQQLEKPKQTVSTEPENASHTKAPIPKARKLIYKSNDLEKDDNQSFPRQRRDSLNARGAPRGILKRNSSS SSTDSETLRLNYNLDPKSKILSPGLTIHERISEKEFSLEDDSSTSSLEPLKHVRFSAVKNELPQSPRPVL GQEVGEFTVLESDQLQNGTEDAGDIEEFQNHPELSHKTPLSHYQLVSSPSDSGREREQLMSSGSAPRDEI PCHSDILPTGPQCVESSSVINGQQEKSSHFTKLPSELSKSPSDELTQCGEPEPSQTADHSFRDHRQGSEE EHSPVLKTLERRAARKLPSKSLEDIPSDSSNQAKVDNLPEELVRSAEDDQKADQEPDTNECIPGISTVPS LPDNQFSHPDKLKRMSKSVPAFLQDESDDRETDTASESSYQLRRYKKSPSSLTNLSSSSGMTSLSSASGS VMSVYSGDFGNLEVKGSVQFALDYVESLKELHVFVAQCKDLAAADVKKQRSDPYVKTYLLPDKGKMGKKK TLVVKKTLNPVYNEILRYKIERQFLKTQKLNLSVWHRDTFKRNSFLGEVELDLETWDWDSKQNKQLKWYP LKRKTAPVALETENRGEMKLALQYVPEPSPGKKLPTTGEVHIWVKECLDLPLLRGSHLNSFVKCTILPDT SRKSRQKTRAVGKTTNPVFNHTMVYDGFRPEDLMEACVELTVWDHYKLTNQFLGGLRIGFGTGKSYGTEV DWMDSTSEEVALWEKMVNSPNTWVEATLPLRMLLIAKLSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289583
ORF Size	2850 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289583.1, NP_001276512.1
RefSeq Size	4779 bp
RefSeq ORF	2853 bp
Locus ID	83671
UniProt ID	Q99N50
MW	107.3 kDa
Gene Summary	Isoform 11 acts as a RAB27A effector protein and plays a role in cytotoxic granule exocytosis in lymphocytes. Required for cytotoxic granule docking at the immunologic synapse. Isoform 1 may play a role in melanosome transport and vesicle trafficking. It controls melanosome distribution in the cell periphery and regulates melanocyte morphology. Isoform 1 acts as a positive mediator of mucus secretion by the surface mucus cells of the stomach. Mediates basal mucus secretion by gastric surface cells by promoting the proper granule biognesis and docking of mucus granules with the apical plasma membrane.[UniProtKB/Swiss-Prot Function]

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