Pacsin3 (NM_001289677) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4921507A02Rik; 6330413E15Rik; AW413130
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229838 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCAGAGGAGGACGCCGGAGGGGAGGTCCTAGGGGGCAGTTTCTGGGAGGCTGGCAACTACAGAC GGACGGTGCAGAGGGTTGAGGATGGGCACAGGCTGTGTGGGGACCTGGTTAGCTGCTTCCAAGAACGTGC CCGCATTGAGAAGGCCTACGCCCAGCAGCTGGCTGACTGGGCCCGCAAGTGGAGGGGTGCTGTGGAGAAG GGCCCACAGTACGGCACCCTGGAGAAGGCCTGGCATGCCTTCTTCACTGCGGCTGAGAGGCTGAGCGAGC TGCACTTGGAGGTGAGGGAGAAGTTGCACGGTCCAGACAGCGAGCGAGTGCGGACCTGGCAGCGAGGGGC TTTCCACCGGCCAGTGCTGGGGGGCTTTCGGGAAAGCCGGGCTGCAGAGGATGGTTTCCGTAAAGCTCAG AAGCCCTGGCTAAAGAGGCTGAAGGAGGTTGAGGCTTCTAAGAAGAGCTACCACACAGCCCGCAAGGATG AGAAGACAGCCCAGACCCGGGAGAGCCACGCGAAGGCAGACAGCTCCATGTCCCAGGAGCAGCTTCGAAA ATTACAGGAGCGGGTGGGCCGCTGCACCAAGGAGGCAGAGAAGATGAAAACCCAGTATGAGCAGACCCTG GCCGAGCTAAATCGCTACACCCCACGCTACATGGAGGACATGGAGCAGGCTTTTGAGAGCTGCCAGGCTG CTGAGCGCCAGCGGCTTCTCTTCTTCAAGGATGTCTTGCTCACCCTGCATCAACACCTCGACCTCTCCAG TAGCGACAAGTTCCATGAACTCCATCGAGACCTGCAGCAGAGCATTGAGGCTGCCAGTGATGAAGAGGAT CTGCGCTGGTGGCGGAGCACACACGGGCCTGGCATGGCCATGAACTGGCCACAGTTTGAGGAATGGTCGT TAGACACACAGAGAGCCATCAGCCGCAAGGAGAAGGGTGGCCGGAGCCCAGATGAGGTTACTCTGACCAG CATTGTACCGACAAGAGATGGCACCGCACCCCCACCCCAGTCCCCATCGTCCCCGGGTAGCGGGCAGGAC GAGGACTGGTCAGATGAGGAGAGTCCCCGGAAAGTTGCCACTGGGGTGAGGGTACGGGCGCTTTATGACT ATGCTGGCCAGGAGGCTGATGAGCTGAGCTTCCGAGCAGGGGAAGAGTTGCTGAAGATGAGCGAGGAGGA TGAGCAGGGCTGGTGCCAGGGCCAGCTGCAGAGTGGCCGCATTGGTCTGTACCCTGCCAATTACGTAGAA TGTGTGGGCGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229838 protein sequence Red=Cloning site Green=Tags(s) MAPEEDAGGEVLGGSFWEAGNYRRTVQRVEDGHRLCGDLVSCFQERARIEKAYAQQLADWARKWRGAVEK GPQYGTLEKAWHAFFTAAERLSELHLEVREKLHGPDSERVRTWQRGAFHRPVLGGFRESRAAEDGFRKAQ KPWLKRLKEVEASKKSYHTARKDEKTAQTRESHAKADSSMSQEQLRKLQERVGRCTKEAEKMKTQYEQTL AELNRYTPRYMEDMEQAFESCQAAERQRLLFFKDVLLTLHQHLDLSSSDKFHELHRDLQQSIEAASDEED LRWWRSTHGPGMAMNWPQFEEWSLDTQRAISRKEKGGRSPDEVTLTSIVPTRDGTAPPPQSPSSPGSGQD EDWSDEESPRKVATGVRVRALYDYAGQEADELSFRAGEELLKMSEEDEQGWCQGQLQSGRIGLYPANYVE CVGA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289677
ORF Size	1275 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289677.1, NP_001276606.1
RefSeq Size	1865 bp
RefSeq ORF	1275 bp
Locus ID	80708
UniProt ID	Q99JB8
MW	48.6 kDa
Gene Summary	Plays a role in endocytosis and regulates internalization of plasma membrane proteins. Overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. Inhibits the TRPV4 calcium channel activity.[UniProtKB/Swiss-Prot Function]

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