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Zap70 (NM_009539) Mouse Tagged Lenti ORF Clone
CAT＃: MR226660L4
- LentiORF®
Lenti ORF clone of Zap70 (mGFP-tagged) - Mouse zeta-chain (TCR) associated protein kinase (Zap70)
"NM_009539" in other vectors (4)
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CNY 6,460.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | mrtle; mur; S; Srk; ZAP-; ZAP-70 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR226660).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009539 |
| ORF Size | 1854 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009539.2, NP_033565.2 |
| RefSeq Size | 2180 bp |
| RefSeq ORF | 1857 bp |
| Locus ID | 22637 |
| UniProt ID | P43404 |
| Gene Summary | This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219715 | Zap70 (untagged) - Mouse zeta-chain (TCR) associated protein kinase (Zap70), (10ug) |
CNY 7,890.00 |
|
| MG226660 | Zap70 (tGFP-tagged) - Mouse zeta-chain (TCR) associated protein kinase (Zap70), (10ug) |
CNY 5,040.00 |
|
| MR226660 | Zap70 (Myc-DDK-tagged) - Mouse zeta-chain (TCR) associated protein kinase (Zap70) |
CNY 4,560.00 |
|
| MR226660L3 | Lenti ORF clone of Zap70 (Myc-DDK-tagged) - Mouse zeta-chain (TCR) associated protein kinase (Zap70) |
CNY 6,460.00 |
