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Xrcc2 (NM_020570) Mouse Tagged ORF Clone
CAT＃: MR223996
- TrueORF®
Xrcc2 (Myc-DDK-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 2 (Xrcc2)
ORF Plasmid: tGFP
"NM_020570" in other vectors (4)
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CNY 2,081.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4921524O04Rik; 8030409M04Rik; RAD51; RecA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223996 representing NM_020570
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTAGCGACTTTCGCAGAGCGGAATCCGGGACAGAGCTCCTTGCCCGACTTGAAGGCAGAAGCTCGT TGAAAGAACTAGAACCCAACCTGTTTGCTGATGAAGATTCACCAGTGCACGGTGATATTTTTGAATTTCA TGGTCCAGAAGGAACGGGGAAAACAGAAATGCTTTATCATTTAACAGCCCGATGTATACTTCCAAAATCA GAGGGTGGACTGCAAATAGAAGTCTTATTTATTGATACAGATTACCACTTTGACATGCTACGGCTCGTGA CAGTTCTTGAACACAGACTCTCGCAAAGCTCCGAGGAAGCCATGAAGCTGTGCCTAGCCAGGTTGTTCCT GGCGTACTGCAGCAGCAGCATGCAGTTACTGCTCACGCTGCACTCCCTGGAAGCCCTGCTCTGCAGTCGC CCCTCTCTCTGCCTTCTCATTGTGGACAGCCTGTCGTCTTTTTACTGGATAGACCGAGTCAGTGGAGGAG AGAGTGTGGCCCTGCAGGAGTCAACGCTGCAGAAGTGCTCTCAGCTCCTAGAGCGGCTTGTCACTGAGTA CCGCTTGCTGCTTTTTGCGACAACGCAGAGTCTAATGCAGAAAGGCTCAGACTCTGCAGACGGGCCTTCT TCCTCCAAGCACCCATGCGATGGAGACATGGGCTACAGAGCCTATCTCTGCAAGGCCTGGCAGAGGGTCG TGAAGCACAGGGTCATCTTCTCCAGGGACGACGAGGCCAAGAGCAGCCGGTTCTCATTAGTTTCACGTCA TTTAAAAAGTAACAGTTTAAAAAAACACAGTTTTATGGTCAGAGAAAGTGGGGTGGAGTTTTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223996 representing NM_020570
Red=Cloning site Green=Tags(s) MCSDFRRAESGTELLARLEGRSSLKELEPNLFADEDSPVHGDIFEFHGPEGTGKTEMLYHLTARCILPKS EGGLQIEVLFIDTDYHFDMLRLVTVLEHRLSQSSEEAMKLCLARLFLAYCSSSMQLLLTLHSLEALLCSR PSLCLLIVDSLSSFYWIDRVSGGESVALQESTLQKCSQLLERLVTEYRLLLFATTQSLMQKGSDSADGPS SSKHPCDGDMGYRAYLCKAWQRVVKHRVIFSRDDEAKSSRFSLVSRHLKSNSLKKHSFMVRESGVEFC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020570 |
| ORF Size | 834 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020570.2, NP_065595.2 |
| RefSeq Size | 3230 bp |
| RefSeq ORF | 837 bp |
| Locus ID | 57434 |
| UniProt ID | Q9CX47 |
| MW | 31.4 kDa |
| Gene Summary | Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210148 | Xrcc2 (untagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 2 (Xrcc2), (10ug) |
CNY 3,990.00 |
|
| MG223996 | Xrcc2 (tGFP-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 2 (Xrcc2), (10ug) |
CNY 2,850.00 |
|
| MR223996L3 | Lenti ORF clone of Xrcc2 (Myc-DDK-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 2 (Xrcc2) |
CNY 4,750.00 |
|
| MR223996L4 | Lenti ORF clone of Xrcc2 (mGFP-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 2 (Xrcc2) |
CNY 4,750.00 |
