Cacna1f (NM_019582) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	A930034B14; Cav1.4; nerg1; nob2; Sfc17
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR222850 representing NM_019582 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGAATCTGAAGTCGGGAAAGATACAACCCCAGAGCCCAGTCCAGCCAATGGGACTGGCCCTGGCC CTGAATGGGGGCTCTGTCCTGGGCCTCCAACTGTGGGGACTGATACCAGCGGGGCGTCAGGCCTGGGGAC CCCAAGAAGAAGGACCCAGCACAACAAACACAAGACTGTGGCGGTGGCCAGTGCTCAGAGATCACCTCGA GCGCTCTTCTGCCTCACCCTTACTAATCCCATTCGTCGGTCCTGCATCAGCATTGTAGAGTGGAAGCCTT TTGATATTCTCATCCTCCTGACAATCTTTGCCAACTGCGTGGCATTGGGGGTATATATCCCCTTCCCTGA GGACGACTCCAACACTGCTAACCACAACTTGGAACAGGTAGAATACGTGTTCCTGGTGATTTTCACCGTG GAGACAGTGCTCAAGATCGTAGCCTATGGGCTGGTGCTCCATCCCAGCGCCTATATTCGCAATGGCTGGA ACCTGCTCGACTTCATCATCGTCGTGGTCGGGCTGTTCAGCGTGCTGCTGGAACAAGGACCTGGGCGGCC AGGAGATGCCCCGCATACTGGAGGAAAGCCAGGAGGCTTCGATGTAAAGGCACTGCGGGCATTTAGGGTG CTACGACCTCTAAGGCTAGTGTCTGGGGTCCCGAGTCTGCACATAGTGCTCAATTCCATCATGAAGGCGC TTGTGCCGCTGCTGCACATTGCCCTGTTGGTGCTCTTCGTCATTATCATTTACGCCATCATCGGACTCGA GCTATTCCTCGGACGAATGCACAAGACATGCTACTTCCTGGGATCTGATATGGAAGCAGAGGAGGACCCA TCACCTTGTGCATCTTCTGGCTCTGGGCGTTCATGCACACTGAACCATACCGAGTGCCGCGGGCGCTGGC CAGGACCCAACGGTGGCATCACGAACTTCGACAATTTTTTCTTTGCCATGCTAACTGTGTTCCAGTGTAT TACCATGGAAGGCTGGACAGACGTCCTCTACTGGATGCAGGATGCCATGGGGTATGAGCTGCCTTGGGTG TACTTTGTGAGCCTTGTCATCTTTGGGTCCTTCTTTGTCCTCAACCTTGTGCTTGGAGTCCTAAGCGGGG AGTTCTCCAAGGAAAGAGAAAAGGCAAAAGCACGAGGTGACTTTCAGAAGCTTCGGGAGAAGCAGCAGAT GGAAGAAGACCTTCGGGGCTACCTGGACTGGATCACACAGGCTGAGGAGTTAGACCTTCATGACCCCTCA GTAGACGGCAACTTGGCTTCTCTTGCTGAAGAGGGACGGGCGGGCCATCGGCCACAACTGTCAGAGCTGA CCAATAGGAGGCGCGGACGGCTGCGATGGTTCAGCCACTCTACTCGCTCCACACACTCCACCAGCAGCCA CGCCAGCCTCCCAGCCAGTGACACTGGCTCCATGACAGACACCCCTGGAGATGAGGATGAAGAAGAGGGG ACCATGGCTAGCTGTACACGCTGCCTAAACAAGATTATGAAAACAAGGATCTGCCGCCACTTCCGCCGAG CCAACCGGGGTCTCCGTGCACGCTGCCGCCGGGCCGTCAAGTCCAACGCCTGCTACTGGGCTGTACTGTT GCTCGTCTTCCTCAACACGTTGACCATAGCTTCAGAGCACCATGGGCAGCCTTTGTGGCTCACCCAGACC CAAGAGTATGCCAACAAAGTTCTGCTCTGCCTCTTCACTGTGGAGATGCTCCTCAAACTGTACGGCCTGG GCCCCTCTGTCTACGTTGCCTCCTTTTTCAACCGCTTTGACTGCTTCGTGGTCTGTGGGGGCATCCTAGA AACCACTTTGGTGGAGGTGGGGGCCATGCAGCCTCTTGGCATCTCAGTGCTCCGATGTGTACGTCTCCTC AGGATCTTCAAGGTCACCAGGCACTGGGCATCCCTGAGCAATCTGGTGGCATCTTTGCTCAATTCCATGA AGTCCATCGCCTCCTTGCTGCTTCTCCTCTTTCTCTTCATCATCATCTTCTCCCTGCTTGGCATGCAGCT GTTTGGGGGCAAGTTCAACTTTGACCAGACCCACACCAAGAGGAGCACCTTTGATACCTTCCCCCAAGCC CTCCTCACTGTCTTTCAGATCCTGACTGGTGAGGATTGGAACGTTGTCATGTATGATGGTATCATGGCCT ACGGTGGGCCCTTCTTCCCAGGGATGCTGGTGTGTGTTTATTTCATCATCCTCTTCATCTGTGGCAACTA CATCCTGCTGAACGTGTTTCTTGCCATTGCCGTGGATAACCTAGCCAGCGGGGATGCAGGCACTGCCAAA GACAAGGGCAGAGAGAAGAGCAGTGAAGGAAACCCTCCAAAGGAGAACAAAGTATTGGTGCCTGGTGGAG AGAATGAGGACGCAAAGGGTGCAAGAAGTGAAGGAGCAGCACCAGGCATGGAGGAGGAGGAGGAGGAGGA AGAAGAAGAAGAGGAGGAGGAGGAAGAGGAAAATGGTGCAGGACATGTGGAACTCTTGCAGGAAGTAGTA CCCAAGGAGAAGGTGGTACCCATCCCTGAAGGCAGTGCCTTCTTCTGCCTTAGCCAAACCAACCCGCTTC GGAAGGCCTGCCACACACTCATACATCACCATATCTTCACCAGTCTCATCCTAGTGTTCATCATCCTCAG TAGTGTGTCCCTGGCTGCTGAGGACCCCATCCGAGCTCACTCCTTCCGAAACCATATTCTGGGATATTTT GATTATGCCTTCACCTCCATATTCACTGTGGAGATTCTACTCAAGATGACAGTGTTTGGGGCCTTCCTGC ACCGAGGCTCTTTCTGCCGTAGCTGGTTCAATCTGTTGGATCTCCTTGTGGTCAGTGTGTCCCTCATCTC CTTCGGCATCCACTCCAGTGCCATCTCAGTTGTGAAGATTCTCCGAGTCCTCCGAGTCCTGCGGCCTCTC CGAGCCATCAACAGAGCCAAGGGACTCAAGCATGTGGTGCAGTGTGTGTTCGTGGCCATCCGGACCATCG GAAACATCATGATTGTCACCACCCTCTTGCAGTTCATGTTCGCCTGCATTGGTGTTCAGCTGTTCAAGGG AAAATTCTACAGTTGCACTGATGAGGCCAAACACACCCTGAAAGAATGCAAGGGCTCCTTCCTCATCTAC CCTGATGGAGATGTGTCACGACCTTTGGTCCGGGAGCGGCTCTGGGTCAACAGTGATTTTAACTTTGACA ACGTCCTTTCAGCCATGATGGCCCTGTTCACTGTCTCTACCTTTGAAGGCTGGCCTGCGCTACTATACAA GGCCATAGATGCAAACGCAGAAGATGAGGGCCCTATCTACAATTACCATGTGGAGATATCAGTATTCTTC ATTGTCTACATCATCATCATCGCCTTCTTCATGATGAACATCTTTGTGGGCTTTGTTATCATCACATTCC GTGCCCAGGGAGAGCAGGAGTACCAAAACTGTGAACTGGACAAGAACCAGCGCCAGTGTGTGGAATATGC CCTCAAAGCTCAGCCACTCCGCCGATACATCCCTAAGAATCCTCATCAGTACCGCGTGTGGGCCACTGTG AACTCTGCTGCCTTTGAGTACCTCATGTTTCTGCTCATCCTGCTCAACACGGTGGCCCTAGCCATGCAGC ACTATGAACAGACTGCTCCCTTTAACTATGCCATGGACATCCTCAACATGGTCTTCACTGGCCTCTTCAC CATTGAGATGGTGCTCAAAATCATCGCCTTTAAACCCAAGCATTACTTTGCAGATGCCTGGAATACGTTT GATGCTCTCATTGTAGTGGGCAGTGTAGTCGACATCGCCGTCACAGAAGTCAATAACGGAGGCCATCTTG GCGAGAGTTCAGAGGACAGCTCCCGCATATCTATCACGTTCTTTCGCCTCTTCCGAGTCATGAGGCTGGT CAAGCTTCTGAGTAAGGGTGAGGGGATCCGCACACTGCTCTGGACATTCATCAAGTCTTTCCAGGCCTTG CCCTATGTGGCACTTCTCATAGCAATGATATTCTTCATCTATGCAGTCATTGGCATGCAGATGTTTGGCA AGGTGGCTCTTCAGGACGGCACGCAGATAAATCGAAACAACAATTTCCAGACCTTTCCGCAGGCTGTGCT GCTTCTGTTCAGGTGTGCCACTGGTGAGGCCTGGCAAGAGATAATGCTAGCCAGCCTTCCAGGAAATCGA TGTGACCCTGAGTCTGACTTTGGCCCAGGCGAGGAATTTACCTGTGGTAGCAGTTTTGCCATCGTCTACT TCATCAGCTTCTTTATGCTCTGTGCCTTCCTGATTATAAATCTCTTTGTGGCTGTAATCATGGATAACTT TGATTACCTAACCAGAGATTGGTCTATCCTGGGACCCCACCACCTTGATGAATTCAAGAGGATCTGGTCT GAATATGACCCCGGAGCCAAGGGCCGCATCAAGCACTTGGATGTGGTTGCCCTGCTGAGACGCATCCAGC CCCCATTGGGATTTGGAAAGCTATGCCCACACCGAGTGGCCTGCAAGAGACTCGTGGCAATGAATGTGCC CCTCAACTCAGATGGAACAGTGACATTCAACGCTACACTCTTTGCCCTGGTGCGGACATCCCTGAAGATC AAGACAGAAGGGAACCTGGATCAAGCCAACCAGGAGCTTCGGATGGTCATCAAAAAGATCTGGAAGCGGA TAAAGCAGAAATTGTTGGATGAGGTCATCCCTCCTCCCGATGAGGAGGAGGTCACTGTGGGAAAATTCTA TGCCACATTCCTGATCCAAGATTATTTCCGAAAATTCCGGAGAAGGAAAGAAAAGGGGCTACTAGGAAGA GAGGCCCCAACAAGCACATCCTCTGCCCTCCAGGCTGGTCTAAGGAGCCTGCAGGACTTGGGTCCTGAGA TCCGTCAAGCCCTCACCTATGACACTGAGGAAGAAGAGGAAGAGGAAGAGGCAGTGGGTCAGGAGGCTGA GGAAGAGGAAGCTGAGAACAACCCAGAACCATACAAAGACTCCATAGACTCCCAGCCCCAATCTCGATGG AACTCTAGGATTTCGGTGTCTCTACCTGTTAAGGAGAAACTTCCAGATTCTCTCTCAACTGGGCCGAGTG ATGATGATGGGCTGGCTCCCAACTCCAGGCAGCCCAGTGTGATACAGGCTGGCTCCCAACCACACAGGAG AAGCTCTGGGGTTTTCATGTTCACTATCCCGGAAGAAGGAAGTATTCAGCTCAAGGGAACTCAAGGGCAG GACAATCAGAATGAGGAACAGGAAGTCCCTGACTGGACTCCTGACCTGGATGAGCAGGCCGGGACTCCTT CGAACCCAGTCCTTTTACCACCTCACTGGTCCCAGCAACACGTAAACGGGCACCATGTGCCACGCCGACG TTTGCTGCCCCCCACGCCTGCAGGTCGGAAGCCCTCCTTCACCATCCAGTGTCTGCAACGCCAGGGCAGT TGTGAAGATTTACCTATCCCAGGCACCTACCATCGTGGACGGACCTCAGGACCAAGCAGGGCTCAGGGTT CCTGGGCAGCCCCTCCTCAGAAGGGTCGACTGCTATATGCCCCCCTGTTGTTGGTGGAGGAATCTACAGT GGGTGAAGGATACCTTGGCAAACTTGGCGGCCCACTGCGTACCTTCACCTGTCTGCAAGTGCCTGGAGCT CATCCGAATCCCAGCCACCGCAAGAGGGGCAGTGCTGACAGTTTGGTGGAGGCTGTGCTCATCTCCGAAG GCCTAGGTCTCTTTGCCCAAGACCCACGATTTGTGGCCCTGGCCAAGCAGGAGATTGCAGATGCATGTCA CCTGACCCTGGATGAGATGGACAGTGCTGCCAGTGACCTGCTGGCACAGAGAACCACCTCCCTTTACAGT GATGAGGAGTCTATTCTTTCCCGCTTTGATGAAGAGGACCTGGGAGATGAGATGGCCTGTGTCCATGCCC TC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR222850 representing NM_019582 Red=Cloning site Green=Tags(s) MSESEVGKDTTPEPSPANGTGPGPEWGLCPGPPTVGTDTSGASGLGTPRRRTQHNKHKTVAVASAQRSPR ALFCLTLTNPIRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTV ETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRV LRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDP SPCASSGSGRSCTLNHTECRGRWPGPNGGITNFDNFFFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWV YFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQMEEDLRGYLDWITQAEELDLHDPS VDGNLASLAEEGRAGHRPQLSELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSMTDTPGDEDEEEG TMASCTRCLNKIMKTRICRHFRRANRGLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPLWLTQT QEYANKVLLCLFTVEMLLKLYGLGPSVYVASFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLL RIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQA LLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCVYFIILFICGNYILLNVFLAIAVDNLASGDAGTAK DKGREKSSEGNPPKENKVLVPGGENEDAKGARSEGAAPGMEEEEEEEEEEEEEEEEENGAGHVELLQEVV PKEKVVPIPEGSAFFCLSQTNPLRKACHTLIHHHIFTSLILVFIILSSVSLAAEDPIRAHSFRNHILGYF DYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPL RAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTLKECKGSFLIY PDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDANAEDEGPIYNYHVEISVFF IVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATV NSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFADAWNTF DALIVVGSVVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQAL PYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNR CDPESDFGPGEEFTCGSSFAIVYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWS EYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNVPLNSDGTVTFNATLFALVRTSLKI KTEGNLDQANQELRMVIKKIWKRIKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGR EAPTSTSSALQAGLRSLQDLGPEIRQALTYDTEEEEEEEEAVGQEAEEEEAENNPEPYKDSIDSQPQSRW NSRISVSLPVKEKLPDSLSTGPSDDDGLAPNSRQPSVIQAGSQPHRRSSGVFMFTIPEEGSIQLKGTQGQ DNQNEEQEVPDWTPDLDEQAGTPSNPVLLPPHWSQQHVNGHHVPRRRLLPPTPAGRKPSFTIQCLQRQGS CEDLPIPGTYHRGRTSGPSRAQGSWAAPPQKGRLLYAPLLLVEESTVGEGYLGKLGGPLRTFTCLQVPGA HPNPSHRKRGSADSLVEAVLISEGLGLFAQDPRFVALAKQEIADACHLTLDEMDSAASDLLAQRTTSLYS DEESILSRFDEEDLGDEMACVHAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_019582
ORF Size	5952 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019582.2, NP_062528.2
RefSeq Size	6075 bp
RefSeq ORF	5955 bp
Locus ID	54652
MW	222.3 kDa

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