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Aph1a (NM_146134) Mouse Tagged Lenti ORF Clone
CAT＃: MR220937L3
- LentiORF®
Lenti ORF clone of Aph1a (Myc-DDK-tagged) - Mouse anterior pharynx defective 1a homolog (C. elegans) (Aph1a), transcript variant 2
"NM_146134" in other vectors (3)
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CNY 4,750.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6530402N02Rik; APH-1a |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR220937).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_146134 |
| ORF Size | 795 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_146134.2, NP_666246.1 |
| RefSeq Size | 2882 bp |
| RefSeq ORF | 798 bp |
| Locus ID | 226548 |
| UniProt ID | Q8BVF7 |
| Gene Summary | This gene encodes a subunit of the gamma-secretase complex, which is localized to the endoplasmic reticulum and golgi apparatus. Gamma-secretase is a multi-protein enzyme that catalyzes intramembraneous proteolysis of type I transmembrane proteins and is essential for many signaling pathways, including the Notch signaling pathway. Studies suggest that the protein encoded by this locus binds directly to substrates of the gamma-secretase complex, including the beta-amyloid precursor protein which is associated with Alzheimer disease progression. This gene is required for normal embryonic development and survival, and disruption is associated with defects in the yolk sack angiogenesis, neural tube formation, and somitogenesis. A pseudogene of this gene is located on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG220937 | Aph1a (tGFP-tagged) - Mouse anterior pharynx defective 1a homolog (C. elegans) (Aph1a) transcript variant 2, (10ug) |
CNY 2,850.00 |
|
| MR220937 | Aph1a (Myc-DDK-tagged) - Mouse anterior pharynx defective 1a homolog (C. elegans) (Aph1a), transcript variant 2 |
CNY 2,081.00 |
|
| MR220937L4 | Lenti ORF clone of Aph1a (mGFP-tagged) - Mouse anterior pharynx defective 1a homolog (C. elegans) (Aph1a), transcript variant 2 |
CNY 4,750.00 |
