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Nt5c1a (NM_001085502) Mouse Tagged ORF Clone
CAT＃: MR217369
- TrueORF®
Nt5c1a (Myc-DDK-tagged) - Mouse 5'-nucleotidase, cytosolic IA (Nt5c1a)
ORF Plasmid: tGFP
"NM_001085502" in other vectors (4)
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CNY 5,488.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cn1a; Gm49 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217369 representing NM_001085502
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCAGGGCAGCCCCGGGAGGCCCGCGAACCCGGGCCAGGGGCAGAGACCGCCGCGGTACCGCGCT GGGAGGAAGCCAAGACTTTCTATGACAACCTCTCGTCCAAGAAGAAACCCAAATCGCCAAAGCCTCAGAA TGCGGTCACCATCGCGGTGTCCTCCAGAGCCTTGTTCCGAATGGATGAGGAACAGAGGATCTACACGGAG CAGGGTGTCGAGGAGTACGTGCGATACCAGCTGGAGCACGAGAATGAGCCCTTCAGCCCCGGGCCTGCCT TCCCCTTTGTGAAGGCCCTGGAGGCTGTGAACAAGCGACTTCGGGAGTTGTACCCTGATAGCGAAGATAT CTTTGACATTGTCCTCATGACCAACAACCATGCTCAGGTGGGAGTTCGTCTCATCAACAGTATCAACCAT TATGACCTGTTCATTGAGCGATTCTGCATGACAGGTGGGAATAGCCCCATCTGCTACCTCAAGGCCTACC ACACCAACCTCTACTTGTCAGCCGATGCAGACAAAGTGCGAGAAGCCATCGATGAGGGGATTGCAGCTGC CACCATCTTCAGCCCCAGCAGGGATGTGGTTGTGTCCCAGAGTCAGCTTCGAGTGGCCTTCGATGGGGAC GCAGTACTCTTCTCCGATGAGTCAGAGCGCATCGTCAAGGCCCATGGGCTGGACAGATTCTTTGAGCATG AGAAGGCCCACGAGAACAAACCTTTGGCGCAGGGCCCCCTAAAGGGCTTCCTGGAAGCACTGGGTAGACT GCAAAAGAAATTCTACTCCAAGGGCCTGCGTCTGGAGTGCCCGATTCGCACGTATTTGGTGACCGCGCGA AGTGCAGCCAGTTCCGGAGCCCGGGCCCTCAAGACCCTGCGCAGCTGGGGCCTGGAGACGGATGAGGCCT TATTCCTAGCTGGAGCACCCAAGGGTCCCCTTCTTGAGAAGATCCGTCCACACATCTTCTTTGATGACCA GATGTTCCACGTGGCTGGAGCTCAGGAGATGGGCACGGTGGCCGCCCATGTGCCTTATGGTGTGGCTCAG AATCCCCGGCGGGCTGCCGCTGCGAAGCAGTCATTAGGTGCACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217369 representing NM_001085502
Red=Cloning site Green=Tags(s) MEPGQPREAREPGPGAETAAVPRWEEAKTFYDNLSSKKKPKSPKPQNAVTIAVSSRALFRMDEEQRIYTE QGVEEYVRYQLEHENEPFSPGPAFPFVKALEAVNKRLRELYPDSEDIFDIVLMTNNHAQVGVRLINSINH YDLFIERFCMTGGNSPICYLKAYHTNLYLSADADKVREAIDEGIAAATIFSPSRDVVVSQSQLRVAFDGD AVLFSDESERIVKAHGLDRFFEHEKAHENKPLAQGPLKGFLEALGRLQKKFYSKGLRLECPIRTYLVTAR SAASSGARALKTLRSWGLETDEALFLAGAPKGPLLEKIRPHIFFDDQMFHVAGAQEMGTVAAHVPYGVAQ NPRRAAAAKQSLGAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001085502 |
| ORF Size | 1095 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001085502.2 |
| RefSeq Size | 1103 bp |
| RefSeq ORF | 1098 bp |
| Locus ID | 230718 |
| UniProt ID | A3KFX0 |
| MW | 41.1 kDa |
| Gene Summary | Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides and has a broad substrate specificity. Helps to regulate adenosine levels in heart during ischemia and hypoxia (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Dynamic compartmentalization of purine nucleotide metabolic enzymes at leading edge in highly motile renal cell carcinoma
,Wolfe, K;Kofuji, S;Yoshino, H;Sasaki, M;Okumura, K;Sasaki, AT;,
Biochem. Biophys. Res. Commun.
,PubMed ID 31196624
[NT5C1A]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212891 | Nt5c1a (untagged) - Mouse 5'-nucleotidase, cytosolic IA (Nt5c1a), (10ug) |
CNY 3,990.00 |
|
| MG217369 | Nt5c1a (tGFP-tagged) - Mouse 5'-nucleotidase cytosolic IA (Nt5c1a), (10ug) |
CNY 4,370.00 |
|
| MR217369L3 | Lenti ORF clone of Nt5c1a (Myc-DDK-tagged) - Mouse 5'-nucleotidase, cytosolic IA (Nt5c1a) |
CNY 5,890.00 |
|
| MR217369L4 | Lenti ORF clone of Nt5c1a (mGFP-tagged) - Mouse 5'-nucleotidase, cytosolic IA (Nt5c1a) |
CNY 5,890.00 |
