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Pak4 (NM_027470) Mouse Tagged ORF Clone
CAT＃: MR209234
- TrueORF®
Pak4 (Myc-DDK-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 4 (Pak4)
ORF Plasmid: tGFP
"NM_027470" in other vectors (4)
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CNY 4,424.00
CNY 4,560.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5730488L07Rik; AW555722; mKIAA1142 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209234 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGGAAAGAAGAAGAAGCGGGTGGAGATCTCAGCCCCATCCAACTTCGAGCACCGTGTACACACAG GCTTTGATCAGCATGAGCAGAAGTTCACAGGGCTGCCCCGCCAGTGGCAGAGCCTGATTGAGGAATCAGC ACGCAGACCCAAGCCCCTGATCGACCCTGCCTGCATCACGTCCATCCAGCCTGGAGCCCCCAAGACCATC GTTCGGGGCAGCAAAGGTGCCAAGGATGGGGCTCTCACTCTGCTGCTTGACGAATTTGAGAACATGTCGG TGACGCGCTCCAACTCCCTACGCAGAGAGAGTCCACCACCACCTGCCCGTGCCCACCAGGAGAATGGAAT GCTGGAGGAGCGGGCAGCCCCGGCCAGAATGGCCCCTGACAAAGCTGGAAGCAGAGCCCGGGCCACAGGC CACAGTGAGGCAGGCAGTGGCAGTGGTGACAGACGGCGGGTGGGGCCAGAGAAAAGGCCCAAATCTTCGA GGGATGGTCCAGGAGGACCCCAGGAGGCCTCCCGCGATAAGCGCCCACTCTCTGGGCCTGATGTCAGCAC TCCTCAGCCTGGCAGTCTGACCAGCGGGACAAAACTAGCAGCTGGCAGGCCCTTTAACACATACCCACGG GCTGACACGGACCACCCACCCCGCGGTGCCCAGGGGGAGCCACATACCATGGCCCCTAATGGGCCTTCAG CCACAGGCCTGGCCGCTCCTCAGTCTTCCTCCTCCTCCCGGCCTCCCACCCGAGCCCGTGGTGCTCCCAG CCCAGGAGTTCTGGGTCCCCATGCCTCTGAGCCCCAGTTGGCCCCCCCAGCACGTGCCCTTGCTGCCCCT GCTGTACCTCCTGCCCCTGGGCCCCCTGGGCCTCGCTCACCACAGCGGGAGCCCCAACGAGTGTCCCATG AGCAGTTCCGGGCTGCCCTGCAGCTGGTGGTGGACCCAGGGGACCCTCGTTCCTATCTAGACAACTTCAT CAAGATTGGTGAGGGCTCTACAGGTATTGTGTGCATTGCCACTGTACGCAGCTCAGGCAAACTGGTGGCC GTCAAGAAGATGGACTTGCGCAAGCAGCAAAGACGTGAACTGCTCTTCAATGAGGTGGTGATCATGCGGG ACTACCGGCACGAGAACGTGGTGGAGATGTACAACAGCTACCTGGTGGGTGACGAACTCTGGGTCGTCAT GGAGTTCCTGGAAGGCGGCGCCCTCACGGATATTGTCACCCACACCAGGATGAACGAGGAACAGATCGCC GCCGTGTGCCTGGCTGTGCTTCAGGCGCTGGCTGTGCTCCACGCCCAGGGTGTCATCCACCGTGACATCA AGAGTGACTCTATCTTGCTGACCCATGATGGCCGGGTGAAGCTGTCCGACTTCGGGTTTTGTGCCCAGGT GAGCAAGGAGGTGCCTCGGAGGAAGTCGCTGGTGGGCACCCCGTATTGGATGGCCCCGGAGCTCATCTCC CGCCTTCCCTATGGGCCAGAGGTGGATATCTGGTCACTGGGGGTGATGGTGATCGAGATGGTGGATGGGG AGCCCCCTTACTTCAACGAGCCACCCCTCAAAGCTATGAAGATGATCCGGGACAACCTCCCGCCCCGATT GAAGAACCTGCACAAGGCGTCACCGTCTCTGAAAGGCTTCTTGGATCGCCTGCTAGTGCGGGACCCGGCC CAGCGGGCCACTGCTGCCGAGCTGCTGAAGCACCCGTTCCTCACCAAGGCGGGGCCACCAGCCAGCATCG TGCCCCTGATGCGCCAGCACCGAACCAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209234 protein sequence
Red=Cloning site Green=Tags(s) MFGKKKKRVEISAPSNFEHRVHTGFDQHEQKFTGLPRQWQSLIEESARRPKPLIDPACITSIQPGAPKTI VRGSKGAKDGALTLLLDEFENMSVTRSNSLRRESPPPPARAHQENGMLEERAAPARMAPDKAGSRARATG HSEAGSGSGDRRRVGPEKRPKSSRDGPGGPQEASRDKRPLSGPDVSTPQPGSLTSGTKLAAGRPFNTYPR ADTDHPPRGAQGEPHTMAPNGPSATGLAAPQSSSSSRPPTRARGAPSPGVLGPHASEPQLAPPARALAAP AVPPAPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKLVA VKKMDLRKQQRRELLFNEVVIMRDYRHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIA AVCLAVLQALAVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELIS RLPYGPEVDIWSLGVMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKASPSLKGFLDRLLVRDPA QRATAAELLKHPFLTKAGPPASIVPLMRQHRTR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027470 |
| ORF Size | 1782 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027470.2 |
| RefSeq Size | 2898 bp |
| RefSeq ORF | 1782 bp |
| Locus ID | 70584 |
| UniProt ID | Q8BTW9 |
| MW | 64.6 kDa |
| Gene Summary | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
PAK4 suppresses motor neuron degeneration in hSOD1G93A -linked amyotrophic lateral sclerosis cell and rat models
,Cong, C;Liang, W;Zhang, C;Wang, Y;Yang, Y;Wang, X;Wang, S;Huo, D;Wang, H;Wang, D;Feng, H;,
Cell proliferation
,PubMed ID 33615605
[PAK4]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219418 | Pak4 (untagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 4 (Pak4), (10ug) |
CNY 7,510.00 |
|
| MG209234 | Pak4 (tGFP-tagged) - Mouse p21 (CDKN1A)-activated kinase 4 (Pak4) |
CNY 5,040.00 |
|
| MR209234L3 | Lenti ORF clone of Pak4 (Myc-DDK-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 4 (Pak4) |
CNY 6,460.00 |
|
| MR209234L4 | Lenti ORF clone of Pak4 (mGFP-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 4 (Pak4) |
CNY 6,460.00 |
