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Plod1 (BC010268) Mouse Tagged ORF Clone
CAT＃: MR205599
- TrueORF®
Plod1 (Myc-DDK-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (cDNA clone MGC:8124 IMAGE:3589182)
ORF Plasmid: tGFP
"BC010268" in other vectors (3)
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CNY 2,090.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Plod, LH1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205599 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGCAGACCTGTGCCGTCAGGACCAAACTTGCACCTACTACTTCAGTGTGGATGCTGATGTGGCTC TGACGGAGCCGAACAGCCTAAGGCTCCTGATTGAACAAAACAAGAATGTCATTGCGCCACTCATGACCCG CCACGGGAGGCTCTGGTCCAACTTTTGGGGGGGTCTGAGTGCAGATGGGTACTACGCCCGCTCAGAGGAC TACGTGGACATTGTGCAGGGCCGTCGTGTTGGCGTCTGGAACGTGCCTTACATCTCAAACATCTACCTGA TCAAGGGCAGTGCCCTGCGGGCTGAACTGCAGAACGTAGACCTTTTCCACTACAGCAAGCTGGATTCCGA CATGAGTTTCTGCGCCAATGTCCGACAGCAGGAGGTGTTCATGTTCCTGACCAACCGGCACACCTTTGGC CACCTGCTTTCCCTGGATAACTACCAGACCACCCACCTACATAATGATCTCTGGGAGGTGTTCAGCAACC CTGAGGACTGGAAAGAAAAGTACATCCATGAGAATTACACCAAGGCCCTGGCGGGGAAGCTGGTGGAGAC GCCTTGTCCGGATGTCTACTGGTTCCCCATCTTCACGGAGGCGGCCTGTGATGAGCTGGTGGAGGAGATG GAACACTATGGCCAGTGGTCTCTGGGTGATAATAAGGACAACCGGATCCAGGGTGGCTACGAAAACGTGC CCACTATCGACATCCATATGAACCAGATCACCTTCGAGCGGGAGTGGCACAAGTTCCTGGTGGAGTACAT CGCCCCCATGACAGAGAAGCTGTACCCTGGCTACTACACTAGGGCCCAGTTTGATCTAGCCTTTGTCGTC CGCTATAAGCCTGATGAGCAGCCTTCCTTGATGCCCCACCATGACGCCTCTACCTTCACCGTCAACATAG CCCTGAACAGGGTTGGGGAAGATTATGAGGGCGGAGGTTGCCGATTTCTGCGCTACAACTGCTCCGTGAG GGCACCAAGGAAGGGCTGGGCCCTCCTGCACCCCGGGCGGCTCACACACTATCATGAGGGGCTTCCTACT ACCAAGGGCACGCGCTACATTGCTGTGTCTTTCGTCGATCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205599 protein sequence
Red=Cloning site Green=Tags(s) MGADLCRQDQTCTYYFSVDADVALTEPNSLRLLIEQNKNVIAPLMTRHGRLWSNFWGGLSADGYYARSED YVDIVQGRRVGVWNVPYISNIYLIKGSALRAELQNVDLFHYSKLDSDMSFCANVRQQEVFMFLTNRHTFG HLLSLDNYQTTHLHNDLWEVFSNPEDWKEKYIHENYTKALAGKLVETPCPDVYWFPIFTEAACDELVEEM EHYGQWSLGDNKDNRIQGGYENVPTIDIHMNQITFEREWHKFLVEYIAPMTEKLYPGYYTRAQFDLAFVV RYKPDEQPSLMPHHDASTFTVNIALNRVGEDYEGGGCRFLRYNCSVRAPRKGWALLHPGRLTHYHEGLPT TKGTRYIAVSFVDP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC010268 |
| ORF Size | 1092 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC010268, AAH10268 |
| RefSeq Size | 1903 bp |
| RefSeq ORF | 1094 bp |
| Locus ID | 18822 |
| MW | 42.3 kDa |
| Gene Summary | Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (PubMed:27119146). Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens (By similarity). These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links (PubMed:27119146).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205599 | Plod1 (tGFP-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (cDNA clone MGC:8124 IMAGE:3589182) |
CNY 2,280.00 |
|
| MR205599L3 | Lenti ORF clone of Plod1 (Myc-DDK-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (cDNA clone MGC:8124 IMAGE:3589182) |
CNY 3,990.00 |
|
| MR205599L4 | Lenti ORF clone of Plod1 (mGFP-tagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (cDNA clone MGC:8124 IMAGE:3589182) |
CNY 3,990.00 |
