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Mfng (NM_008595) Mouse Tagged ORF Clone
CAT＃: MR204632
- TrueORF®
Mfng (Myc-DDK-tagged) - Mouse MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng)
"NM_008595" in other vectors (3)
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CNY 1,416.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW546563 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR204632 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTGCCGACTTTTTCGGGGCATGGCGGGAGCCCTCTTTACCCTCCTGTGCGTGGGGCTCCTGTCTC TACGATACCACTCAAGTTTGTCCCAGAGGATGATACAGGGCGCGCTCAGGCTGAACCAACGGAACCCAGG ACCCCTGGAGCTGCAGCTAGGCGACATCTTCATCGCAGTCAAGACTACCTGGGCCTTCCATCGCTCCCGC CTGGACCTGCTACTAGACACGTGGGTCTCCAGGATCAGGCAACAGACATTCATCTTCACTGACAGCCCAG ATGAACGCCTCCAGGAGAGACTAGGCCCGCACCTCGTGGTCACCAACTGTTCTGCAGAGCACAGTCATCC TGCTCTGTCCTGCAAGATGGCTGCAGAGTTCGATGCCTTCTTGGTCAGTGGCCTCAGGTGGTTCTGCCAC GTGGATGATGACAACTATGTGAACCCCAAGGCTCTGCTGCAGCTGTTGAAAACATTCCCGCAGGACCGTG ATGTCTATGTGGGCAAGCCCAGCCTGAACCGGCCCATCCACGCCTCTGAGCTGCAGTCAAAAAACCGCAC GAAGCTGGTGCGGTTCTGGTTTGCCACAGGGGGTGCTGGTTTCTGCATCAACCGCCAACTGGCTTTGAAG ATGGTGCCATGGGCCAGCGGCTCCCACTTTGTGGACACTTCTGCTCTCATCCGGCTCCCCGATGACTGCA CTGTGGGCTACATCATCGAGTGCAAGCTGGGGGGTCGCCTGCAGCCCAGCCCCCTCTTCCACTCACACCT GGAAACCCTGCAGCTGCTGGGGGCTGCCCAGCTTCCGGAGCAGGTCACCCTCAGCTACGGTGTCTTTGAG GGGAAACTGAATGTCATCAAGCTACCGGGCCCCTTCTCCCATGAAGAGGACCCCTCCAGATTCCGCTCCC TCCATTGTCTCCTCTACCCAGACACACCCTGGTGTCCGCTGCTGGCAGCGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204632 protein sequence
Red=Cloning site Green=Tags(s) MHCRLFRGMAGALFTLLCVGLLSLRYHSSLSQRMIQGALRLNQRNPGPLELQLGDIFIAVKTTWAFHRSR LDLLLDTWVSRIRQQTFIFTDSPDERLQERLGPHLVVTNCSAEHSHPALSCKMAAEFDAFLVSGLRWFCH VDDDNYVNPKALLQLLKTFPQDRDVYVGKPSLNRPIHASELQSKNRTKLVRFWFATGGAGFCINRQLALK MVPWASGSHFVDTSALIRLPDDCTVGYIIECKLGGRLQPSPLFHSHLETLQLLGAAQLPEQVTLSYGVFE GKLNVIKLPGPFSHEEDPSRFRSLHCLLYPDTPWCPLLAAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008595 |
| ORF Size | 966 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008595.1, NM_008595.2, NP_032621.1 |
| RefSeq Size | 1834 bp |
| RefSeq ORF | 966 bp |
| Locus ID | 17305 |
| UniProt ID | O09008 |
| MW | 36.2 kDa |
| Gene Summary | Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in inhibition of NOTCH1 activation by JAG1 and enhancement of NOTCH1 activation by DLL1 via an increase in its binding to DLL1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200915 | Mfng (untagged) - Mouse MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), (10ug) |
CNY 2,000.00 |
|
| MR204632L3 | Lenti ORF clone of Mfng (Myc-DDK-tagged) - Mouse MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng) |
CNY 4,750.00 |
|
| MR204632L4 | Lenti ORF clone of Mfng (mGFP-tagged) - Mouse MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng) |
CNY 4,750.00 |
