United States
Germany
Japan
United Kingdom
China
Drosha (NM_001130149) Mouse Tagged ORF Clone
CAT＃: MG227001
- TrueORF®
Drosha (tGFP-tagged) - Mouse ribonuclease III nuclear (Rnasen) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001130149" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 13,300.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1110013A17Rik; AI874853; Etohi2; Rn3; Rnasen |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227001 representing NM_001130149
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAGGCAATACGTGTCATAGAATGTCGTACCACCCAGGACGAGGGTGTCCCCGGGGCCGAGGAGGAC ACGGAGCCAGACCTTCAGCACCAGCTTTCAGACCCCAAAACCTGCGACTTCTTCATCCCCAGCAGCCGCC TGCGCAGTATCAATATGAGCCTCCGAGCGCCCCCTCTTCCTCCTACTCGAACTCTCAGGCCCCCAGCTTT ATGCCCCCACGGCCAGACTTTGTCCCTTACCCTCCCCCAGCGGCGCCGTCTGCCCAAGGGCCTCTCCCTC CCTGCCCAGTGAGGCCGCCTTACCCCAACCACCAGATGAGACACCCTTTCCCGGTGCCTCCCTGTTTTCC ACCCATGCCCCCTCCGATGCCTTGTCCCAATAACCCGCCTGCCTCCGGAGCACCTCCCGGACAAGGCACT TTCCCCTTCATGGTGCCCCCTCCTTCCATGCCCCACCCTCCGCCCCCGCCCGTCATGCCGCAGCAGGTTA ATTACCAGTACCCCCCTGGGTACTCGCACAGCTTCCCACCGCCCGGCTTCAACAGTTACCAGAACAACTC CAGCTCTTTCCCACCCAGTGCTAACAGCAGCAGCACTCCTCATTTTCGACACCTCCCACCATACTCACTC CCAAAGGCTCAGAATGAGAGGCGGTCCCCAGAAAGGCTCAAGCACTACGACGACCACAGGCACCGAGATC ACAGTCACGGGCGAGGCGAGAGGCATCGGTCCCTGGAGCGCAGGGAGCGCGGCCGCAGCCCTGAAAGGAG AAGACCTGAGAGCCGCTACCGCTCAGACTATGATCGGGGGAGAACGCCACCGCCTCGCCACCGCAGCTAT GAAAGGAGCAGAGAGCGGGATCGAGAGAGACACAGGCACCGGGAGGCCCGCAGATCACCGTCTCTAGAAA GGTCCTACAAGAAAGAGTATAAGAGATCTGGAAGGAGTTACGCTTTACCAGTTGCTCCTGAGCCCGCTGG GTGCACACCAGAGTTGCCTGGGGAGATGATTAAAACTACAGAGTCTTGGGCCCCGCCCCCGGAGAATGTG AATCATCGTTCTCCAAGCAGGGAGAAGAAGAGAGCTCGTTGGGAGGAGGAAAAAGACAGATGGAGCGACA GCCAGGGCTCTGGCAAAGAGAAGAACTACACGTCCATCAAAGAGAAAGAGGCAGAGGAGGTGCCTCCAGA GAAGACGGAGGAGGAGGAGGAAGAGCTCCTTAAGCCTGTGTGGATTCGCTGTACACATTCGGAAAGCTAT TACTCCAGTGACCCAATGGATCAGGTGGGAGACTCGACTGTCGTAGGGACAAGCAGGCTCCGTGATTTGT ATGACAAATTTGAGGAAGAATTGGGGAATAGGCAAGAGAAGGCCAAAGCTGCCCGGCCTCCGTGGGAGCC TCCGAAGACAAAGCTGGATGAAGATTTAGAGAGTTCCAGTGAGTCTGAATGTGAGACTGACGACGACAGC ACCTGTTCGAGCAGCTCGGACTCGGAGGTGTTCGATGTCATTGCAGAGATTAAACGCAAAAAGGCTCACC CTGACCGGCTTCATGATGAACTCTGGTACAACGACCCAGGCCAGATGAACGATGGACCGCTTTGCAAATG CAGTGCAAAAGCCAGACGCACAGGAATCCGCCACAGCATTTATCCCGGAGAAGAGGCAATCAAGCCCTGC CGTCCGATGACCAACAACGCTGGCCGGCTTTTCCACTATCGGATCACCGTCTCCCCGCCTACCAACTTCT TAACTGACAGGCCAACAGTCATAGAATATGATGACCACGAGTATATTTTTGAAGGATTTTCTATGTTTGC ACATGCTCCTCTGACCAATATTCCACTGTGTAAAGTGATTCGATTCAACATAGACTACACGATTCATTTT ATCGAAGAGATGATGCCTGAGAATTTTTGTGTGAAAGGACTTGAACTGTTTTCATTGTTCCTATTCAGAG ATATTTTGGAATTATATGACTGGAATCTTAAAGGTCCTTTGTTTGAAGACAGCCCTCCCTGCTGTCCGAG ATTTCATTTCATGCCACGTTTTGTAAGATTTCTTCCAGATGGAGGCAAAGAAGTGTTATCCATGCACCAG ATCCTTCTCTACCTGCTGCGCTGCAGCAAGGCTCTGGTGCCCGAGGAGGAGATTGCCAACATGCTCCAGT GGGAGGAGCTCGAGTGGCAGAAATACGCAGAGGAGTGCAAAGGCATGATCGTTACCAACCCTGGGACGAA ACCGAGCTCTGTCCGCATTGATCAGCTGGATCGTGAACAGTTCAACCCCGAAGTGATCACTTTCCCGATT ATCGTTCACTTTGGGATCCGCCCCGCACAGTTGAGTTATGCAGGAGACCCACAGTATCAGAAATTATGGA AAAGCTATGTGAAACTTCGCCACCTCCTAGCAAACAGTCCCAAAGTCAAACAAACTGACAAGCAGAAGCT GGCTCAGAGGGAGGAAGCGCTCCAGAAAATACGGCAGAAGAACACGATGCGGCGAGAAGTCACCGTGGAG CTGAGTAGCCAAGGATTCTGGAAAACTGGCATTCGTTCTGATGTCTGTCAGCATGCAATGATGCTGCCTG TTCTGACCCATCACATCCGGTACCATCAATGCTTAATGCACCTGGACAAGTTGATAGGATATACTTTCCA AGACCGTTGTCTGCTCCAGCTTGCCATGACTCATCCGAGTCACCATTTAAATTTTGGAATGAATCCTGAC CATGCCAGGAATTCTTTGTCTAACTGTGGAATTCGACAACCCAAATATGGAGACAGAAAAGTTCATCACA TGCACATGCGGAAAAAAGGAATTAATACCCTAATAAATATCATGTCACGCCTTGGCCAAGATGATCCAAC TCCTTCAAGGATTAATCACAACGAAAGGTTGGAGTTCCTGGGGGATGCTGTTGTTGAGTTTCTGACCAGT GTCCACCTGTACTACCTGTTTCCTAGCTTGGAGGAAGGGGGCTTGGCGACCTATCGGACCGCCATTGTTC AGAATCAGCACCTCGCCATGCTTGCAAAGAAACTTGAACTGGATCGATTTATGCTATATGCCCATGGGCC CGACCTATGTAGAGAATCAGATCTCCGCCATGCGATGGCCAATTGTTTTGAAGCCTTGATAGGAGCTGTT TACTTGGAGGGGAGCCTAGAGGAAGCCAAACAGTTATTTGGACGCTTACTCTTTAATGATCCGGACCTTC GAGAAGTCTGGCTCAATTATCCTCTCCACCCACTCCAACTACAAGAGCCAAATACGGATCGGCAACTTAT TGAAACTTCCCCAGTTCTACAGAAACTTACGGAGTTTGAAGAAGCAATTGGAGTGATCTTCACTCACGTC CGGCTTCTGGCGAGGGCGTTCACACTGAGAACCGTGGGCTTTAACCACCTGACCCTAGGCCACAATCAGA GGATGGAATTTCTGGGCGACTCCATAATGCAGCTGGTGGCCACAGAGTACTTGTTCATTCATTTCCCGGA CCATCACGAAGGACACTTGACGTTGTTACGAAGTTCCTTAGTGAACAACAGAACGCAAGCCAAGGTAGCA GAGGAACTGGGCATGCAGGAGTATGCCATCACCAACGACAAAACCAAGAGACCCGTGGCCCTAAGAACCA AGACTTTGGCAGACCTTTTGGAATCATTTATCGCAGCGCTGTACATCGACAAGGACCTGGAATATGTCCA CACTTTCATGAACGTCTGCTTCTTTCCCCGGCTGAAGGAGTTCATTCTGAATCAGGATTGGAACGACCCC AAGTCGCAGCTGCAGCAGTGTTGTCTGACCCTGAGGACAGAAGGGAAAGAGCCTGACATCCCCTTATACA AGACTCTGCAGACAGTGGGGCCATCCCATGCTAGAACCTACACTGTGGCTGTTTATTTCAAGGGAGAAAG GATAGGCTGTGGGAAAGGACCAAGCATTCAGCAGGCGGAGATGGGAGCAGCAATGGATGCACTGGAGAAA TATAACTTTCCCCAGATGGCCCATCAGAAGCGGTTCATTGAGCGGAAATACAGACAAGAGTTAAAGGAAA TGAGGTGGGAAAGAGAGCATCAGGAGAGAGAGCCGGAGGAGGCTGAAGACATCAAGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227001 representing NM_001130149
Red=Cloning site Green=Tags(s) MQGNTCHRMSYHPGRGCPRGRGGHGARPSAPAFRPQNLRLLHPQQPPAQYQYEPPSAPSSSYSNSQAPSF MPPRPDFVPYPPPAAPSAQGPLPPCPVRPPYPNHQMRHPFPVPPCFPPMPPPMPCPNNPPASGAPPGQGT FPFMVPPPSMPHPPPPPVMPQQVNYQYPPGYSHSFPPPGFNSYQNNSSSFPPSANSSSTPHFRHLPPYSL PKAQNERRSPERLKHYDDHRHRDHSHGRGERHRSLERRERGRSPERRRPESRYRSDYDRGRTPPPRHRSY ERSRERDRERHRHREARRSPSLERSYKKEYKRSGRSYALPVAPEPAGCTPELPGEMIKTTESWAPPPENV NHRSPSREKKRARWEEEKDRWSDSQGSGKEKNYTSIKEKEAEEVPPEKTEEEEEELLKPVWIRCTHSESY YSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGNRQEKAKAARPPWEPPKTKLDEDLESSSESECETDDDS TCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHSIYPGEEAIKPC RPMTNNAGRLFHYRITVSPPTNFLTDRPTVIEYDDHEYIFEGFSMFAHAPLTNIPLCKVIRFNIDYTIHF IEEMMPENFCVKGLELFSLFLFRDILELYDWNLKGPLFEDSPPCCPRFHFMPRFVRFLPDGGKEVLSMHQ ILLYLLRCSKALVPEEEIANMLQWEELEWQKYAEECKGMIVTNPGTKPSSVRIDQLDREQFNPEVITFPI IVHFGIRPAQLSYAGDPQYQKLWKSYVKLRHLLANSPKVKQTDKQKLAQREEALQKIRQKNTMRREVTVE LSSQGFWKTGIRSDVCQHAMMLPVLTHHIRYHQCLMHLDKLIGYTFQDRCLLQLAMTHPSHHLNFGMNPD HARNSLSNCGIRQPKYGDRKVHHMHMRKKGINTLINIMSRLGQDDPTPSRINHNERLEFLGDAVVEFLTS VHLYYLFPSLEEGGLATYRTAIVQNQHLAMLAKKLELDRFMLYAHGPDLCRESDLRHAMANCFEALIGAV YLEGSLEEAKQLFGRLLFNDPDLREVWLNYPLHPLQLQEPNTDRQLIETSPVLQKLTEFEEAIGVIFTHV RLLARAFTLRTVGFNHLTLGHNQRMEFLGDSIMQLVATEYLFIHFPDHHEGHLTLLRSSLVNNRTQAKVA EELGMQEYAITNDKTKRPVALRTKTLADLLESFIAALYIDKDLEYVHTFMNVCFFPRLKEFILNQDWNDP KSQLQQCCLTLRTEGKEPDIPLYKTLQTVGPSHARTYTVAVYFKGERIGCGKGPSIQQAEMGAAMDALEK YNFPQMAHQKRFIERKYRQELKEMRWEREHQEREPEEAEDIKK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001130149 |
| ORF Size | 4119 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001130149.1, NP_001123621.1 |
| RefSeq Size | 4584 bp |
| RefSeq ORF | 4122 bp |
| Locus ID | 14000 |
| UniProt ID | Q5HZJ0 |
| Gene Summary | Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224355 | Drosha (untagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 1, (10ug) |
CNY 17,580.00 |
|
| MR227001 | Drosha (Myc-DDK-tagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 1 |
CNY 9,392.00 |
|
| MR227001L3 | Lenti ORF clone of Drosha (Myc-DDK-tagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 1 |
CNY 13,970.00 |
|
| MR227001L4 | Lenti ORF clone of Drosha (mGFP-tagged) - Mouse drosha, ribonuclease type III (Drosha), transcript variant 1 |
CNY 13,970.00 |
