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Auts2 (NM_177047) Mouse Tagged ORF Clone
CAT＃: MG225787
- TrueORF®
Auts2 (tGFP-tagged) - Mouse autism susceptibility candidate 2 (Auts2), (10ug)
ORF Plasmid: DDK
"NM_177047" in other vectors (4)
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CNY 14,768.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2700063G02Rik; A730011F23Rik; D830032G16Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225787 representing NM_177047
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGGCCCGACGCGGGGACATGGACTCCGGAAAAAGCGGAGGTCTCGATCACAGCGAGACCGGGAGA GACGCTCCCGGGCCGGGTTAGGAACCGGTGCGGCGGGTGGTATAGGAGCCGGCCGGACCCGGGCTCCCTC GCTGGCTTCATCGTCGGGCTCCGACAAGGAAGACAATGGAAAGCCCCCATCCTCCGCCCCGTCCCGGCCC AGACCCCCGCGGAGGAAGCGCAGAGAGTCCACCTCAGCCGAAGAGGACATCATTGATGGATTTGCCATGA CCAGCTTTGTTACTTTTGAAGCGCTGGAGAAAGATGTAGCAGTTAAGCCGCAGGAACGTGCGGAGAAACG ACAGACTCCCCTGACCAAGAAAAAACGAGAAGCTCTTACCAATGGTTTGTCTTTTCACTCAAAGAAGAGC CGGCTCAGCCACTCACACCACTACAGCTCAGATCGAGAGAATGACCGCAATCTCTGCCAGCACCTTGGGA AGAGAAAGAAGATGCCTAAGGGACTCAGACAGCTCAAGCCAGGACAGAACAGCTGCAGGGACAGTGACAG CGAAAGCGCTAGCGGAGAATCCAAGGGCTTCCAGCGGAGCAGCTCTCGGGAGAGGCTCAGTGATAGTTCA GCTCCTTCCAGCTTGGGGACAGGCTACTTCTGCGACAGTGACAGCGACCAGGAAGAGAAGGCTTCAGATG CTAGCTCTGAAAAACTCTTCAACACTGTTCTTGTAAACAAAGATCCAGAGTTAGGTGTTGGCGCACTACC TGAACACAACCAAGATGCGGGACCAATTGTCCCCAAGATCTCGGGTCTAGAGAGAAGCCAGGAGAAGAGC CAGGACTGTTGCAAAGAGCCCGTCTTCGAACCCGTGGTGCTCAAAGACCCACATCCTCAGCTCCCTCAGC TACCATCCCAGGCCCAGGCCGAACCTCAGCTTCAGATTCCATCTCCAGGCCCAGACTTGGTACCGCGCAC TGAAGCTCCGCCCCAGTTTCCTCCTCCAAGTACACAGCCAGCCCAAGGACCCCCAGAAGCCCAGCTGCAG CCTGCCCCGCTGCCTCAGGTGCAGCAGCGGCCACCCCGGCCCCAGTCCCCCAGCCACCTGCTCCAGCAGA CTCTTCCGCCTGTGCAGTCACATCCCTCCTCCCAGAGCCTCTCACAGCCACTGTCGGCCTACAACAGCAG TAGCCTGAGCCTCAACAGCTTAAGCAGCAGAAGCAGTACCCCGGCAAAAACCCAGCCCGCCCCTCCTCAC ATCTCCCACCACCCGTCCGCCTCTCCCTTCCCCCTCTCCCTGCCCAACCACAGCCCTCTGCACAGCTTCA CACCCACCCTCCAGCCCCCTGCCCACTCACATCACCCCAATATGTTCGCCCCTCCCACTGCTCTGCCTCC TCCCCCGCCGCTGACGTCTGGAAGTCTGCAGGTGCCAGGGCATCCCGCAGGGAGCACGTACTCAGAACAA GATATCCTGAGGCAGGAACTGAACACGCGTTTCCTGGCCTCTCAGAGTGCCGACCGAGGGGCCTCCCTGG GCCCTCCACCCTACCTGCGGACCGAGTTCCATCAGCACCAGCATCAGCACCAGCACACCCATCAGCACAC GCACCAGCATACCTTCACACCGTTCCCCCACGCCATCCCGCCCACTGCCATCATGCCGACGCCAGCACCT CCCATGTTTGACAAATACCCCACCAAAGTTGACCCATTCTACCGGCATAGTCTCTTCCACTCCTATCCTC CTGCGGTCTCGGGTATACCCCCCATGATCCCACCCACGGGCCCTTTCGGGTCACTCCAAGGAGCATTCCA GCCGAAGACATCCAACCCTATCGACGTGGCTGCCCGGCCTGGAACAGTCCCACATACTCTGCTCCAGAAG GACCCAAGGTTGACAGATCCTTTCAGACCTATGCTAAGGAAACCAGGAAAGTGGTGCGCCATGCATGTGC ATATCGCCTGGCAGATTTATCACCATCAACAAAAAGTCAAGAAACAGATGCAGTCAGACCCACACAAGCT GGACTTTGGGCTGAAGCCTGAGTTCCTGAGCCGTCCTCCAGGCCCTAGTCTCTTCGGAGCCATCCATCAC CCCCACGATCTGGCACGACCTTCCACTTTATTCTCTGCCGCTGGTGCTGCACATCCTACTGGGACCCCTT TTGGGCCACCACCTCATCACAGTAACTTTCTCAACCCTGCCGCTCACCTAGAGCCTTTTAACCGGCCGTC TACGTTCACGGGCCTCGCTGCAGTTGGTGGCAATGCCTTCGGGGGACTTGGAAACCCTTCAGTTACACCC AACTCAGTGTTCGGCCACAAGGATAGCCCCAGTGTGCAGAACTTTAGCAATCCTCACGAACCCTGGAACC GGCTGCACCGAACGCCTCCGTCGTTCCCGACCCCTCCGCCCTGGCTGAAGCCAGGGGAATTGGAGCGCAG TGCGTCCGCTGCAGCCCATGACAGAGATAGAGATGTAGATAAACGAGACTCCTCCGTTAGTAAAGATGAC AAGGAAAGGGAAAGCGTTGAGAAAAGACACCCCAGCCATCCTTCCCCAGCACCTCCCGTCCCGGTGAGCG CCCTGGGCCATAACCGCAGCTCCACCGATCCGACGACCAGGGGTCATTTGAATACTGAGGCTCGTGAGAA AGATAAACCAAAAGAGAAGGAGAGAGACCACTCGGGATCCCGAAAGGACCTGACCACCGAAGAGCACAAA GCCAAGGAAAGCCATCTGCCCGAGAGAGATGGGCACAGCCACGAGGGACGTGCAGCAGGCGAGGAACCCA AGCAGCTGTCCCGGGTGCCATCGCCCTACGTGAGGACACCAGGCGTGGATAGCACCAGGCCTAACAGCAC CTCTAGTCGGGAAGCCGAGCCACGCAAGGGTGAGCCGGCCTACGAGAACCCCAAGAAGAACGCCGAGGTC AAGGTCAAAGAGGAACGCAAGGAAGATCACGACCTACCTACTGAGGCCCCCCAGGCCCACAGGACCTCAG AGGCACCTCCGCCCAGCTCCTCAGCCAGTGCCAGCGTGCACCCAGGGCCTCTGGCATCCATGCCCATGAC CGTGGGAGTGACTGGCATCCATGCCATGAACAGTATCGGTAGTCTGGACAGGACACGTATGGTGACCCCA TTTATGGGCCTCAGTCCGATCCCAGGAGGAGAGCGTTTCCCATACCCCTCTTTCCACTGGGACCCTATGC GGGACCCCTTGAGGGATCCTTACCGTGACTTGGACATGCACCGAAGAGACCCTCTGGGCAGGGACTTTCT GCTCAGGAATGACCCCCTGCACCGGCTCTCCACACCCCGGTTGTATGAAGCCGACCGATCGTTCCGGGAC AGGGAGCCTCATGATTACAGCCACCACCACCACCACCACCATCACCCGCTGGCCGTGGACCCTCGACGGG AGCACGAACGAGGAGGACACCTGGATGAACGCGAGCGGCTGCACGTGCTTCGGGAGGACTATGAGCACCC GCGGTTGCACCCTGTGCACCCCGCTTCTCTAGATGGACACCTCCCACACCCGAGCCTGCTCACACCAGGC CTGCCTAGTATGCACTACCCTCGCATCAGCCCTACTGCGGGCCACCAGAATGGACTGCTTAACAAGACCC CGCCCACTGCGGCGCTTAGCGCCCCACCTCCGCTCATCTCCACATTGGGGGGCCGCCCGGGATCCCCCCG GAGGACTACGCCTCTGTCGGCTGAAATCCGAGAGAGGCCACCGTCCCACACACTGAAGGACATTGAAGCT CGG ACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225787 representing NM_177047
Red=Cloning site Green=Tags(s) MDGPTRGHGLRKKRRSRSQRDRERRSRAGLGTGAAGGIGAGRTRAPSLASSSGSDKEDNGKPPSSAPSRP RPPRRKRRESTSAEEDIIDGFAMTSFVTFEALEKDVAVKPQERAEKRQTPLTKKKREALTNGLSFHSKKS RLSHSHHYSSDRENDRNLCQHLGKRKKMPKGLRQLKPGQNSCRDSDSESASGESKGFQRSSSRERLSDSS APSSLGTGYFCDSDSDQEEKASDASSEKLFNTVLVNKDPELGVGALPEHNQDAGPIVPKISGLERSQEKS QDCCKEPVFEPVVLKDPHPQLPQLPSQAQAEPQLQIPSPGPDLVPRTEAPPQFPPPSTQPAQGPPEAQLQ PAPLPQVQQRPPRPQSPSHLLQQTLPPVQSHPSSQSLSQPLSAYNSSSLSLNSLSSRSSTPAKTQPAPPH ISHHPSASPFPLSLPNHSPLHSFTPTLQPPAHSHHPNMFAPPTALPPPPPLTSGSLQVPGHPAGSTYSEQ DILRQELNTRFLASQSADRGASLGPPPYLRTEFHQHQHQHQHTHQHTHQHTFTPFPHAIPPTAIMPTPAP PMFDKYPTKVDPFYRHSLFHSYPPAVSGIPPMIPPTGPFGSLQGAFQPKTSNPIDVAARPGTVPHTLLQK DPRLTDPFRPMLRKPGKWCAMHVHIAWQIYHHQQKVKKQMQSDPHKLDFGLKPEFLSRPPGPSLFGAIHH PHDLARPSTLFSAAGAAHPTGTPFGPPPHHSNFLNPAAHLEPFNRPSTFTGLAAVGGNAFGGLGNPSVTP NSVFGHKDSPSVQNFSNPHEPWNRLHRTPPSFPTPPPWLKPGELERSASAAAHDRDRDVDKRDSSVSKDD KERESVEKRHPSHPSPAPPVPVSALGHNRSSTDPTTRGHLNTEAREKDKPKEKERDHSGSRKDLTTEEHK AKESHLPERDGHSHEGRAAGEEPKQLSRVPSPYVRTPGVDSTRPNSTSSREAEPRKGEPAYENPKKNAEV KVKEERKEDHDLPTEAPQAHRTSEAPPPSSSASASVHPGPLASMPMTVGVTGIHAMNSIGSLDRTRMVTP FMGLSPIPGGERFPYPSFHWDPMRDPLRDPYRDLDMHRRDPLGRDFLLRNDPLHRLSTPRLYEADRSFRD REPHDYSHHHHHHHHPLAVDPRREHERGGHLDERERLHVLREDYEHPRLHPVHPASLDGHLPHPSLLTPG LPSMHYPRISPTAGHQNGLLNKTPPTAALSAPPPLISTLGGRPGSPRRTTPLSAEIRERPPSHTLKDIEA R TRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-NotI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177047 |
| ORF Size | 3783 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177047.3, NP_796021.2 |
| RefSeq Size | 6060 bp |
| RefSeq ORF | 3786 bp |
| Locus ID | 319974 |
| UniProt ID | A0A087WPF7 |
| Gene Summary | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The PRC1-like complex that contains PCGF5, RNF2, CSNK2B, RYBP and AUTS2 has decreased histone H2A ubiquitination activity, due to the phosphorylation of RNF2 by CSNK2B. As a consequence, the complex mediates transcriptional activation (By similarity). In the cytoplasm, plays a role in axon and dendrite elongation and in neuronal migration during embryonic brain development. Promotes reorganization of the actin cytoskeleton, lamellipodia formation and neurite elongation via its interaction with RAC guanine nucleotide exchange factors, which then leads to the activation of RAC1 (PubMed:25533347).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224100 | Auts2 (untagged) - Mouse autism susceptibility candidate 2 (Auts2), (10ug) |
CNY 16,250.00 |
|
| MR225787 | Auts2 (Myc-DDK-tagged) - Mouse autism susceptibility candidate 2 (Auts2) |
CNY 13,168.00 |
|
| MR225787L3 | Lenti ORF clone of Auts2 (Myc-DDK-tagged) - Mouse autism susceptibility candidate 2 (Auts2) |
CNY 14,440.00 |
|
| MR225787L4 | Lenti ORF clone of Auts2 (mGFP-tagged) - Mouse autism susceptibility candidate 2 (Auts2) |
CNY 14,440.00 |
