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Inpp5f (NM_178641) Mouse Tagged ORF Clone
CAT＃: MG224434
- TrueORF®
Inpp5f (tGFP-tagged) - Mouse inositol polyphosphate-5-phosphatase F (Inpp5f), (10ug)
ORF Plasmid: DDK
"NM_178641" in other vectors (4)
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CNY 10,830.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 5830435P03Rik; AI115354; AW561896; cI-27; hSAC2; mKIAA0966; SAC2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224434 representing NM_178641
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTCTTTCAGGCTAAGGACCACTACATCCTGCAGCAGGGCGAGCGTGCCCTGTGGTGCAGCCGGC GCGACGGCGGCCTGCAGCTCCGACCGGCCACGGATCTGCTTCTTGCCTGGAACCCCATTTGTTTGGGATT AGTGGAAGGTGTAATTGGGAAAATTCAACTTCATTCAGATCTTCCATGGTGGCTTATTTTAATTCGGCAG AAAGCGTTGGTAGGCAAACTCCCAGGAGACCATGAGGTCTGTAAAGTCACCAAGATTGCTGTATTGTCAC TTTCTGAAATGGAACCTCAGGAGCTTGAGCTGGAGCTCTGTAAGAAGCATCATTTTGGAATTAACAAGCC TGAGAAGATTATACCATCTCCTGACGACTCCAAGTTTCTACTGAAGACATTTACAAATATCAAATCCAAC GTGTCTGCTCCCAACAAAAAGAAAGTTAAAGAAAGTAAAGAAAAGGAGAAACTGGAGCGGCGTTTGCTTG AGGAGTTGCTGAAGATGTTCATGGACTCAGAGTCCTTCTATTATAGCTTGACCTATGACCTGACCAACTC GGTGCAGAGACAGAGCACTGGGGAGAGGGATGGCCGTCCCCTGTGGCAGAAGGTTGATGACCGATTTTTC TGGAATAAGTACATGATCCAAGCTCTCACTGAGATTGGTACTCCGGATGTGGACTTCTGGATCATCCCCA TTATCCAGGGTTTTGTGCAGATTGAAGAACTTGTGGTTAATTACAATGAGTCCTCGGATGATGACAAGAG CAGCCCAGAGACGCCCCCTCAGGACTCCACTTGTGTAGATGACATTCACCCACGATTTCTAGTGGCTCTT ATATCTCGAAGAAGTAGGCACAGAGCAGGAATGCGGTATAAACGAAGAGGAGTGGATAAAAATGGAAATG TTGCGAATTATGTGGAGACTGAGCAGTTAATCCATGTTCATCATCATACCCTGTCATTTATCCAAACTCG AGGCTCTGTGCCGGTCTTTTGGAGCCAGGTTGGGTATCGCTATAATCCAAGACCTCGGCTAGACAAAAGT GAGAAGGAAACGGTTGACTGTTTCTGTGCCCATTTTGAAGAACAACTGAAAATTTACAAAAAACAGGTTA TTGTTAACTTGGTAGACCAGGCTGGAAGGGAGAAGATTATTGGTGATGCTTACCTGAAGCAGGTGCTGCT CTTCAACAACCCCAAGCTCACCTATGTCTCCTTCGATTTCCATGAGCACTGCCGAGGAATGAAGTTCGAG AATGTTCAAACCCTCACGGATGCCATTCATGACATCATCATTGACATGAAGTGGTGTTGGGTTGATCAAG CTGGTGTGATATGTAAGCAAGAAGGGATTTTTCGAGTTAATTGCATGGACTGCCTGGATCGCACCAACGT GGTCCAGGCTGCCATAGCGCGCGTGGTCATGGAACAGCAGCTTAAAAAACTAGGTGTGATGCCTCCAGAG CAGCCACTACCTGTGAAATGTAATCGGACCTACCAGATAATGTGGGCCAACAACGGTGACTCCATCAGCA GGCAGTATGCTGGGACTGCTGCTCTAAAGGGTGACTTTACAAGAACAGGAGAAAGGAAGTTGGCAGGAGT TATGAAAGATGGCGTTAACTCGGCAAATAGGTACTACCTCAGTCGGTTCAAGGATGCCTACAGGCAAGCC GTCATAGATCTGATGCAAGGCGTCCCAGTGACAGAAGACCTTTACTCCATATTTACCAAGGAGAAGGAGC ATGAAGCTTTGCATAAGGAGAGCCAGAGAAGCCACCAGGAGCTCATCAGCCAGCTCTTACAGAGTTATAT GCAGTTGCTGCTGCCGGGTGACGAGAAGTTCCACGGGGGCTGGGCCCTGGTTGACTGCGACCCCAGCCTC ACTGACGCTGCGCACAGAGATGTGGAAGTGCTGCTGCTGCTGTCTAACGCTGCCTACTATGTGGCCTATT ATGATGACGAAGTTGATAAAGTAAACCAGTATCAACGACTGGGCCTAGAAGACCTGGAAAGAATAGAAAT AGGTCCTGAACCTACTCTTTTCGGCAAACCCAAGTTCTCGTGCATGCGGCTGCACTACAGGTGTAAAGAA GCAGGCGGTTACTTCCATACGCTAAGAGCTGTGCCACGTAGTCCTGAAGAAGATGGCAAAGACACTCTTC AGTGCATCGCAGAGATGCTGCAGATCACCAAGCAAGCCATGGGACTGGACGTTCCTATCATTGAGAAGAA ACTTGAGAGGAAAAGCAGTAAGCCTCATGAAGACATCATTGGTATCAGATCTCAAAACCAAGGCTCTCTT GCCCAGGGGAAAAGCTTTTTAATGAGCAAATTTTCATCTCTAAATCAAAAAGTAAAGCAGACCAAATCCA ATGTGAACATCGGCAACCTACGAAAATTAGGAAACTTTACAAAGCCTGAAATGAAAGTTAACTTTCTAAA ACCAAACTTAAAAGTCAATCTTTGGAAGTCAGATAGTAGTCTTGAGACCATGGAAAACCCAGGAGTGATG GGTAATAAGGTCCAGGGGGAGTCTGATGGGGACATTTCTTCAGATAACGACTCGTACCACTCTGATGAAT TCCTTACAAATTCTAAGTCGGAGGAAGACAAGCAGCTAGCCAATTCTTTAGAGAGCGTAGGGCCAATAGA TTACATTCTCCCAAGCTGTGGGATTATTGTGTCAGCACCTCGATTAGGCAGTCGGTCTCAGTCTGCCAGC AGTATCGACGTCAGCACTCATGCCCCCTCAGAAGCTGCTGCTGGTCCTGGAAGTGAACTTGGGAAAGGCC TGGAGTCTCCCCTGAAGAAAAGCCCTTCTGCTGACAGCATACACACACGGACTGGCTTCACAAAGCCCAT GGATGTTTACTGCCAGAGATTTGTGCAGGATGCACAGAACAAAATGAATGACCTGTCAGAGATCAGGTCT GTAGCTCAGAAGAGTGAGGAGGGAAGTCACAAGACTAACCGTGTGTCTAATGAAGAAACCCAGTCAGAAC CAATGGGACAGACGCCTCCTCGACCCTCTCAGTTAAACGTCTCTTGTTCTGTGGCAGGCCCACCATTCTT ATCAGTTGAACCAGTACATTCAGTGCTATCTCAGAAGACGCCCAGCTCCGGGTCCAGCCTTCTGGAACTT GAGGCAGGGCTCTGCGTAACTCCCTCTTCAGAGAGCAGCAGCAGCAGGGCAGTCTCTCCCTTTGCAAAGA TCCGCAGCTCCATGGTCCAAGTCGCTAATATTACCCAAGCTGGGCTAACTCATGGGATAAACTTGGCGGT TGCCAAAGTTCAGAAGAGTCCAGCAGAACCCGAAGCGGTTAACGAAATTCAACAAAATGAACTTAAAAAT ATGTTTACACAATGCCAGACACGAATAATTCAGATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224434 representing NM_178641
Red=Cloning site Green=Tags(s) MELFQAKDHYILQQGERALWCSRRDGGLQLRPATDLLLAWNPICLGLVEGVIGKIQLHSDLPWWLILIRQ KALVGKLPGDHEVCKVTKIAVLSLSEMEPQELELELCKKHHFGINKPEKIIPSPDDSKFLLKTFTNIKSN VSAPNKKKVKESKEKEKLERRLLEELLKMFMDSESFYYSLTYDLTNSVQRQSTGERDGRPLWQKVDDRFF WNKYMIQALTEIGTPDVDFWIIPIIQGFVQIEELVVNYNESSDDDKSSPETPPQDSTCVDDIHPRFLVAL ISRRSRHRAGMRYKRRGVDKNGNVANYVETEQLIHVHHHTLSFIQTRGSVPVFWSQVGYRYNPRPRLDKS EKETVDCFCAHFEEQLKIYKKQVIVNLVDQAGREKIIGDAYLKQVLLFNNPKLTYVSFDFHEHCRGMKFE NVQTLTDAIHDIIIDMKWCWVDQAGVICKQEGIFRVNCMDCLDRTNVVQAAIARVVMEQQLKKLGVMPPE QPLPVKCNRTYQIMWANNGDSISRQYAGTAALKGDFTRTGERKLAGVMKDGVNSANRYYLSRFKDAYRQA VIDLMQGVPVTEDLYSIFTKEKEHEALHKESQRSHQELISQLLQSYMQLLLPGDEKFHGGWALVDCDPSL TDAAHRDVEVLLLLSNAAYYVAYYDDEVDKVNQYQRLGLEDLERIEIGPEPTLFGKPKFSCMRLHYRCKE AGGYFHTLRAVPRSPEEDGKDTLQCIAEMLQITKQAMGLDVPIIEKKLERKSSKPHEDIIGIRSQNQGSL AQGKSFLMSKFSSLNQKVKQTKSNVNIGNLRKLGNFTKPEMKVNFLKPNLKVNLWKSDSSLETMENPGVM GNKVQGESDGDISSDNDSYHSDEFLTNSKSEEDKQLANSLESVGPIDYILPSCGIIVSAPRLGSRSQSAS SIDVSTHAPSEAAAGPGSELGKGLESPLKKSPSADSIHTRTGFTKPMDVYCQRFVQDAQNKMNDLSEIRS VAQKSEEGSHKTNRVSNEETQSEPMGQTPPRPSQLNVSCSVAGPPFLSVEPVHSVLSQKTPSSGSSLLEL EAGLCVTPSSESSSSRAVSPFAKIRSSMVQVANITQAGLTHGINLAVAKVQKSPAEPEAVNEIQQNELKN MFTQCQTRIIQI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178641 |
| ORF Size | 3396 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178641.5, NP_848756.2 |
| RefSeq Size | 4740 bp |
| RefSeq ORF | 3399 bp |
| Locus ID | 101490 |
| UniProt ID | Q8CDA1 |
| Gene Summary | Inositol 4-phosphatase which mainly acts on phosphatidylinositol 4-phosphate. May be functionally linked to OCRL, which converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol, for a sequential dephosphorylation of phosphatidylinositol 4,5-bisphosphate at the 5 and 4 position of inositol, thus playing an important role in the endocytic recycling (PubMed:25869668, PubMed:25869669). Regulator of TF:TFRC and integrins recycling pathway, is also involved in cell migration mechanisms (By similarity). Modulates AKT/GSK3B pathway by decreasing AKT and GSK3B phosphorylation (PubMed:17322895). Negatively regulates STAT3 signaling pathway through inhibition of STAT3 phosphorylation and translocation to the nucleus (By similarity). Functionally important modulator of cardiac myocyte size and of the cardiac response to stress (PubMed:19875726). May play a role as negative regulator of axon regeneration after central nervous system injuries (PubMed:26203138).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223693 | Inpp5f (untagged) - Mouse inositol polyphosphate-5-phosphatase F (Inpp5f), (10ug) |
CNY 14,540.00 |
|
| MR224434 | Inpp5f (Myc-DDK-tagged) - Mouse inositol polyphosphate-5-phosphatase F (Inpp5f) |
CNY 7,888.00 |
|
| MR224434L3 | Lenti ORF clone of Inpp5f (Myc-DDK-tagged) - Mouse inositol polyphosphate-5-phosphatase F (Inpp5f) |
CNY 11,780.00 |
|
| MR224434L4 | Lenti ORF clone of Inpp5f (mGFP-tagged) - Mouse inositol polyphosphate-5-phosphatase F (Inpp5f) |
CNY 11,780.00 |
