United States
Germany
Japan
United Kingdom
China
Flt3 (NM_010229) Mouse Tagged ORF Clone
CAT＃: MG223373
- TrueORF®
Flt3 (tGFP-tagged) - Mouse FMS-like tyrosine kinase 3 (Flt3), (10ug)
ORF Plasmid: DDK
"NM_010229" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 12,584.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | B230315G04; CD135; Flk-2; Flk2; Flt-3; Ly72; wmfl |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223373 representing NM_010229
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGCGTTGGCGCAGCGCAGCGACCGGCGGCTGCTGCTGCTTGTTGTTTTGTCAGTAATGATTCTTG AGACCGTTACAAACCAAGACCTGCCTGTGATCAAGTGTGTTTTAATCAGTCATGAGAACAATGGCTCATC AGCGGGAAAGCCATCATCGTACCGAATGGTGCGAGGATCCCCAGAAGACCTCCAGTGTGCCCCGAGGCGC CAGAGTGAAGGGACGGTATATGAAGCGGCCACCGTGGAGGTGGCCGAGTCTGGGTCCATCACCCTGCAAG TGCAGCTCGCCACCCCAGGGGACCTTTCCTGCCTCTGGGTCTTTAAGCACAGCTCCCTGGGCTGCCAGCC GCACTTTGATTTACAAAACAGAGGAATCGTTTCCATGGCCATCTTGAACGTGACAGAGACCCAGGCAGGA GAATACCTACTCCATATTCAGAGCGAAGCCGCCAACTACACAGTACTGTTCACAGTGAATGTAAGAGATA CACAGCTGTACGTGCTAAGAAGACCTTACTTTAGGAAGATGGAAAACCAGGACGCACTGCTCTGCATCTC CGAGGGTGTTCCAGAGCCCACTGTGGAGTGGGTGCTCTGCAGCTCCCACAGGGAAAGCTGTAAAGAAGAA GGCCCTGCTGTTGTCAGAAAGGAGGAAAAGGTACTTCATGAGTTGTTCGGAACAGACATCAGATGCTGTG CTAGAAATGCACTGGGCCGCGAATGCACCAAGCTGTTCACCATAGATCTAAACCAGGCTCCTCAGAGCAC ACTGCCCCAGTTATTCCTGAAAGTGGGGGAACCCTTGTGGATCAGGTGTAAGGCCATCCATGTGAACCAT GGATTCGGGCTCACCTGGGAGCTGGAAGACAAAGCCCTGGAGGAGGGCAGCTACTTTGAGATGAGTACCT ACTCCACAAACAGGACCATGATTCGGATTCTCTTGGCCTTTGTGTCTTCCGTGGGAAGGAACGACACCGG ATATTACACCTGCTCTTCCTCAAAGCACCCCAGCCAGTCAGCGTTGGTGACCATCCTAGAAAAAGGGTTT ATAAACGCTACCAGCTCGCAAGAAGAGTATGAAATTGACCCGTACGAAAAGTTCTGCTTCTCAGTCAGGT TTAAAGCGTACCCACGAATCCGATGCACGTGGATCTTCTCTCAAGCCTCATTTCCTTGTGAACAGAGAGG CCTGGAGGATGGGTACAGCATATCTAAATTTTGCGATCATAAGAACAAGCCAGGAGAGTACATATTCTAT GCAGAAAATGATGACGCCCAGTTCACCAAAATGTTCACGCTGAATATAAGAAAGAAACCTCAAGTGCTAG CAAATGCCTCAGCCAGCCAGGCGTCCTGTTCCTCTGATGGCTACCCGCTACCCTCTTGGACCTGGAAGAA GTGTTCGGACAAATCTCCCAATTGCACGGAGGAAATCCCAGAAGGAGTTTGGAATAAAAAGGCTAACAGA AAAGTGTTTGGCCAGTGGGTGTCGAGCAGTACTCTAAATATGAGTGAGGCCGGGAAAGGGCTTCTGGTCA AATGCTGTGCGTACAATTCTATGGGCACGTCTTGCGAAACCATCTTTTTAAACTCACCAGGCCCCTTCCC TTTCATCCAAGACAACATCTCCTTCTATGCGACCATTGGGCTCTGTCTCCCCTTCATTGTTGTTCTCATT GTGTTGATCTGCCACAAATACAAAAAGCAATTTAGGTACGAGAGTCAGCTGCAGATGATCCAGGTGACTG GCCCCCTGGATAACGAGTACTTCTACGTTGACTTCAGGGACTATGAATATGACCTTAAGTGGGAGTTCCC GAGAGAGAACTTAGAGTTTGGGAAGGTCCTGGGGTCTGGCGCTTTCGGGAGGGTGATGAACGCCACGGCC TATGGCATTAGTAAAACGGGAGTCTCAATTCAGGTGGCGGTGAAGATGCTAAAAGAGAAAGCTGACAGCT GTGAAAAAGAAGCTCTCATGTCGGAGCTCAAAATGATGACCCACCTGGGACACCATGACAACATCGTGAA TCTGCTGGGGGCATGCACACTGTCAGGGCCAGTGTACTTGATTTTTGAATATTGTTGCTATGGTGACCTC CTCAACTACCTAAGAAGTAAAAGAGAGAAGTTTCACAGGACATGGACAGAGATTTTTAAGGAACATAATT TCAGTTTTTACCCTACTTTCCAGGCACATTCAAATTCCAGCATGCCTGGTTCACGAGAAGTTCAGTTACA CCCGCCCTTGGATCAGCTCTCAGGGTTCAATGGGAATTTAATTCATTCTGAAGATGAGATTGAATATGAA AACCAGAAGAGGCTGGCAGAAGAAGAGGAGGAAGATTTGAACGTGCTGACGTTTGAAGACCTCCTTTGCT TTGCGTACCAAGTGGCCAAAGGCATGGAATTCCTGGAGTTCAAGTCGTGTGTCCACAGAGACCTGGCAGC CAGGAATGTGTTGGTCACCCACGGGAAGGTGGTGAAGATCTGTGACTTTGGACTGGCCCGAGACATCCTG AGCGACTCCAGCTACGTCGTCAGGGGCAACGCACGGCTGCCGGTGAAGTGGATGGCACCTGAGAGCTTAT TTGAAGGGATCTACACAATCAAGAGTGACGTCTGGTCCTACGGCATCCTTCTCTGGGAGATATTTTCACT GGGTGTGAACCCTTACCCTGGCATTCCTGTCGACGCTAACTTCTATAAACTGATTCAGAGTGGATTTAAA ATGGAGCAGCCATTCTATGCCACAGAAGGGATATACTTTGTAATGCAATCCTGCTGGGCTTTTGACTCAA GGAAGCGGCCATCCTTCCCCAACCTGACTTCATTTTTAGGATGTCAGCTGGCAGAGGCAGAAGAAGCGAT GTATCAGAACATGGGTGGCAACGTCCCAGAACATCCATCCATCTACCAAAACAGGCGGCCCCTCAGCAGA GAGGCAGGCTCAGAGCCGCCATCGCCACAGGCCCAGGTGAAGATTCACGGAGAAAGAAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223373 representing NM_010229
Red=Cloning site Green=Tags(s) MRALAQRSDRRLLLLVVLSVMILETVTNQDLPVIKCVLISHENNGSSAGKPSSYRMVRGSPEDLQCAPRR QSEGTVYEAATVEVAESGSITLQVQLATPGDLSCLWVFKHSSLGCQPHFDLQNRGIVSMAILNVTETQAG EYLLHIQSEAANYTVLFTVNVRDTQLYVLRRPYFRKMENQDALLCISEGVPEPTVEWVLCSSHRESCKEE GPAVVRKEEKVLHELFGTDIRCCARNALGRECTKLFTIDLNQAPQSTLPQLFLKVGEPLWIRCKAIHVNH GFGLTWELEDKALEEGSYFEMSTYSTNRTMIRILLAFVSSVGRNDTGYYTCSSSKHPSQSALVTILEKGF INATSSQEEYEIDPYEKFCFSVRFKAYPRIRCTWIFSQASFPCEQRGLEDGYSISKFCDHKNKPGEYIFY AENDDAQFTKMFTLNIRKKPQVLANASASQASCSSDGYPLPSWTWKKCSDKSPNCTEEIPEGVWNKKANR KVFGQWVSSSTLNMSEAGKGLLVKCCAYNSMGTSCETIFLNSPGPFPFIQDNISFYATIGLCLPFIVVLI VLICHKYKKQFRYESQLQMIQVTGPLDNEYFYVDFRDYEYDLKWEFPRENLEFGKVLGSGAFGRVMNATA YGISKTGVSIQVAVKMLKEKADSCEKEALMSELKMMTHLGHHDNIVNLLGACTLSGPVYLIFEYCCYGDL LNYLRSKREKFHRTWTEIFKEHNFSFYPTFQAHSNSSMPGSREVQLHPPLDQLSGFNGNLIHSEDEIEYE NQKRLAEEEEEDLNVLTFEDLLCFAYQVAKGMEFLEFKSCVHRDLAARNVLVTHGKVVKICDFGLARDIL SDSSYVVRGNARLPVKWMAPESLFEGIYTIKSDVWSYGILLWEIFSLGVNPYPGIPVDANFYKLIQSGFK MEQPFYATEGIYFVMQSCWAFDSRKRPSFPNLTSFLGCQLAEAEEAMYQNMGGNVPEHPSIYQNRRPLSR EAGSEPPSPQAQVKIHGERS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010229 |
| ORF Size | 3000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010229.2, NP_034359.2 |
| RefSeq Size | 3664 bp |
| RefSeq ORF | 3003 bp |
| Locus ID | 14255 |
| UniProt ID | Q00342 |
| Gene Summary | Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223148 | Flt3 (untagged) - Mouse FMS-like tyrosine kinase 3 (Flt3), (10ug) |
CNY 6,968.00 |
|
| MR223373 | Flt3 (Myc-DDK-tagged) - Mouse FMS-like tyrosine kinase 3 (Flt3) |
CNY 10,984.00 |
|
| MR223373L3 | Lenti ORF clone of Flt3 (Myc-DDK-tagged) - Mouse FMS-like tyrosine kinase 3 (Flt3) |
CNY 11,880.00 |
|
| MR223373L4 | Lenti ORF clone of Flt3 (mGFP-tagged) - Mouse FMS-like tyrosine kinase 3 (Flt3) |
CNY 11,880.00 |
