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Sf3b3 (NM_133953) Mouse Tagged ORF Clone
CAT＃: MG211816
- TrueORF®
Sf3b3 (tGFP-tagged) - Mouse splicing factor 3b, subunit 3 (Sf3b3)
ORF Plasmid: DDK
"NM_133953" in other vectors (4)
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CNY 10,550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1810061H24Rik; 5730409A01Rik; AA409318; D8Ertd633e; mKIAA0017; RSE1; SAP130 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211816 representing NM_133953
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCTGTACAACCTGACCTTACAGCGGGCCACTGGCATCAGCTTTGCCATCCATGGCAACTTCTCTG GAACCAAGCAACAGGAAATTGTTGTTTCCCGTGGGAAGATTTTGGAGTTACTACGCCCAGACCCTAACAC TGGCAAAGTACATACCCTCCTCACTGTTGAAGTATTTGGTGTTATCCGGTCACTTATGGCGTTTAGGCTA ACAGGTGGCACCAAAGACTACATTGTAGTTGGCAGTGACTCAGGCCGGATTGTTATCTTGGAATACCAGC CATCAAAGAATATGTTTGAGAAAATTCACCAAGAAACTTTTGGCAAAAGCGGATGTCGCCGTATTGTTCC TGGCCAGTTCTTAGCTGTGGATCCCAAAGGAAGAGCTGTTATGATTAGTGCAATTGAGAAGCAGAAATTG GTATATATCTTGAACAGAGACGCTGCAGCCCGACTTACCATTTCCTCTCCCCTGGAGGCCCACAAAGCGA ACACTCTGGTGTATCACGTGGTTGGCGTAGACGTGGGATTTGAGAATCCTATGTTTGCTTGTCTGGAAAT GGATTATGAGGAGGCAGACAATGATCCAACAGGGGAAGCAGCAGCCAATACCCAGCAGACACTTACTTTC TATGAACTGGACCTTGGCTTAAATCATGTGGTTCGGAAATACAGTGAACCCTTGGAGGAACATGGCAACT TCCTTATTACAGTTCCTGGAGGGTCAGATGGTCCAAGTGGAGTGCTAATCTGCTCTGAGAATTATATCAC CTACAAGAACTTTGGTGACCAGCCAGACATCCGATGTCCAATTCCCAGGAGACGGAATGACCTGGATGAC CCTGAACGAGGAATGATTTTTGTTTGCTCTGCCACCCATAAGACCAAGTCCATGTTCTTCTTTCTGGCCC AAACGGAGCAGGGAGATATCTTTAAGATCACTTTAGAGACAGATGAAGATATGGTAACCGAGATCCGCCT CAAGTATTTTGATACTGTGCCTGTTGCTGCTGCTATGTGTGTGCTTAAAACAGGCTTCCTTTTTGTGGCG TCAGAATTTGGAAATCATTACTTGTATCAAATTGCCCATCTCGGAGATGATGATGAAGAACCTGAGTTTT CGTCTGCCATGCCTCTAGAAGAAGGAGACACATTCTTCTTTCAGCCGAGACCACTCAAAAATCTCGTGCT GGTGGATGAACTGGACAGCCTCTCTCCTATTCTGTTTTGCCAGATAGCTGATTTGGCCAATGAAGACACC CCACAGCTGTATGTAGCCTGTGGTAGGGGACCCCGGTCCTCCCTGAGAGTCTTAAGGCATGGACTTGAGG TGTCGGAAATGGCTGTTTCTGAGCTCCCTGGTAACCCCAATGCTGTCTGGACAGTCCGTCGGCACATTGA AGATGAGTTTGATGCCTACATCATTGTGTCTTTCGTGAATGCCACACTTGTGCTATCTATCGGAGAGACT GTGGAAGAAGTGACTGACTCTGGCTTCTTGGGCACCACTCCAACCTTGTCCTGCTCCTTGTTAGGAGATG ATGCACTGGTGCAGGTGTATCCAGATGGCATCCGACACATACGAGCAGATAAAAGAGTTAATGAGTGGAA GACTCCCGGCAAGAAAACGATTGTGAAGTGTGCAGTGAACCAGCGACAAGTGGTGATCGCCCTGACTGGG GGAGAGCTGGTCTATTTCGAGATGGATCCTTCAGGACAGTTGAATGAGTACACGGAGCGGAAAGAGATGT CAGCAGATGTGGTGTGCATGAGTCTGGCCAACGTGCCCCCTGGAGAACAGCGATCTCGGTTCCTGGCTGT GGGGCTGGTGGACAACACTGTCAGAATCATCTCCCTCGACCCATCAGACTGTTTACAGCCTCTGAGTATG CAGGCTCTCCCAGCCCAGCCTGAGTCTCTATGTATTGTGGAAATGGGTGGGACTGAGAAACAAGATGAGC TGGGTGAGAGAGGCTCTATTGGCTTCCTGTACCTGAACATTGGACTACAGAACGGTGTACTGCTGAGGAC TGTCCTAGACCCTGTTACTGGGGACCTGTCTGACACCCGCACTAGGTATCTGGGGTCCCGTCCTGTGAAA CTCTTCCGGGTCCGGATGCAAGGCCAGGAGGCTGTTCTGGCTATGTCCAGTCGCTCATGGCTGAGCTACT CTTACCAGTCTCGCTTCCATCTCACCCCTCTGTCTTATGAGACCCTGGAATTTGCATCGGGCTTTGCCTC TGAACAGTGTCCCGAGGGCATTGTTGCCATCTCCACCAACACTTTGAGGATTTTGGCTTTAGAGAAGCTT GGTGCTGTCTTCAATCAAGTAGCCTTCCCATTGCAGTACACACCCAGGAAGTTTGTCATCCACCCTGAGA GTAACAACCTTATCATCATTGAGACGGATCACAATGCCTACACTGAGGCCACAAAAGCTCAGAGAAAGCA GCAGATGGCTGAGGAAATGGTAGAAGCAGCAGGGGAGGATGAGCGGGAGTTGGCTGCAGAAATGGCAGCA GCATTCCTCAATGAAAACCTCCCAGAATCCATTTTTGGAGCTCCCAAGGCTGGCAATGGGCAGTGGGCCT CTGTGATTCGGGTGATGAACCCTATCCAGGGGAATACACTAGACCTTGTCCAGTTGGAGCAAAACGAGGC AGCCTTTAGTGTGGCAGTGTGTAGGTTCTCCAACACTGGTGAAGATTGGTATGTGCTGGTGGGTGTTGCC AAGGACTTGATACTGAGCCCTCGATCTGTGGCAGGGGGCTTTGTGTATACGTACAAGCTTGTGAACAACG GGGAGAAGTTGGAGTTTCTGCACAAGACTCCAGTGGAAGAGGTCCCTGCTGCCATTGCCCCATTCCAGGG GAGGGTACTGATTGGTGTCGGGAAGCTTTTACGAGTTTATGACCTGGGGAAGAAGAAGTTACTTCGAAAG TGTGAGAATAAGCATATTGCCAATTACATATCTGGGATCCAGACAATTGGTCACAGGGTAATTGTGTCTG ATGTCCAAGAAAGTTTCATCTGGGTTCGATACAAGCGTAACGAGAACCAGCTCATTATCTTTGCTGATGA CACCTATCCCAGATGGGTCACCACAGCCAGCCTTCTCGACTATGACACTGTGGCTGGGGCAGACAAGTTT GGCAACATTTGTGTGGTGAGGCTCCCACCTAACACCAACGATGAGGTGGATGAAGATCCCACTGGAAACA AAGCCCTGTGGGACCGGGGCTTGCTCAATGGGGCTTCACAGAAGGCAGAGGTCATCATGAACTACCACGT TGGAGAGACCGTGCTGTCGCTACAGAAGACCACACTGATCCCTGGAGGCTCAGAGTCCCTTGTCTATACC ACTTTATCTGGAGGAATTGGCATCCTTGTCCCTTTCACGTCTCACGAGGACCATGACTTCTTCCAGCATG TTGAAATGCACCTGCGGTCTGAGCATCCCCCTCTTTGTGGACGGGACCACCTGAGCTTTCGTTCTTACTA TTTCCCTGTTAAGAATGTGATTGATGGAGACCTTTGTGAGCAGTTCAACTCCATGGAACCCAACAAGCAG AAGAACGTCTCTGAGGAGCTGGACCGAACCCCACCTGAAGTGTCCAAGAAGCTTGAGGACATTAGGACCC GCTATGCCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211816 representing NM_133953
Red=Cloning site Green=Tags(s) MFLYNLTLQRATGISFAIHGNFSGTKQQEIVVSRGKILELLRPDPNTGKVHTLLTVEVFGVIRSLMAFRL TGGTKDYIVVGSDSGRIVILEYQPSKNMFEKIHQETFGKSGCRRIVPGQFLAVDPKGRAVMISAIEKQKL VYILNRDAAARLTISSPLEAHKANTLVYHVVGVDVGFENPMFACLEMDYEEADNDPTGEAAANTQQTLTF YELDLGLNHVVRKYSEPLEEHGNFLITVPGGSDGPSGVLICSENYITYKNFGDQPDIRCPIPRRRNDLDD PERGMIFVCSATHKTKSMFFFLAQTEQGDIFKITLETDEDMVTEIRLKYFDTVPVAAAMCVLKTGFLFVA SEFGNHYLYQIAHLGDDDEEPEFSSAMPLEEGDTFFFQPRPLKNLVLVDELDSLSPILFCQIADLANEDT PQLYVACGRGPRSSLRVLRHGLEVSEMAVSELPGNPNAVWTVRRHIEDEFDAYIIVSFVNATLVLSIGET VEEVTDSGFLGTTPTLSCSLLGDDALVQVYPDGIRHIRADKRVNEWKTPGKKTIVKCAVNQRQVVIALTG GELVYFEMDPSGQLNEYTERKEMSADVVCMSLANVPPGEQRSRFLAVGLVDNTVRIISLDPSDCLQPLSM QALPAQPESLCIVEMGGTEKQDELGERGSIGFLYLNIGLQNGVLLRTVLDPVTGDLSDTRTRYLGSRPVK LFRVRMQGQEAVLAMSSRSWLSYSYQSRFHLTPLSYETLEFASGFASEQCPEGIVAISTNTLRILALEKL GAVFNQVAFPLQYTPRKFVIHPESNNLIIIETDHNAYTEATKAQRKQQMAEEMVEAAGEDERELAAEMAA AFLNENLPESIFGAPKAGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTGEDWYVLVGVA KDLILSPRSVAGGFVYTYKLVNNGEKLEFLHKTPVEEVPAAIAPFQGRVLIGVGKLLRVYDLGKKKLLRK CENKHIANYISGIQTIGHRVIVSDVQESFIWVRYKRNENQLIIFADDTYPRWVTTASLLDYDTVAGADKF GNICVVRLPPNTNDEVDEDPTGNKALWDRGLLNGASQKAEVIMNYHVGETVLSLQKTTLIPGGSESLVYT TLSGGIGILVPFTSHEDHDFFQHVEMHLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQ KNVSEELDRTPPEVSKKLEDIRTRYAF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133953 |
| ORF Size | 3651 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133953.2, NP_598714.1 |
| RefSeq Size | 4299 bp |
| RefSeq ORF | 3654 bp |
| Locus ID | 101943 |
| UniProt ID | Q921M3 |
| Gene Summary | Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205619 | Sf3b3 (untagged) - Mouse splicing factor 3b, subunit 3 (Sf3b3), (10ug) |
CNY 8,480.00 |
|
| MR211816 | Sf3b3 (Myc-DDK-tagged) - Mouse splicing factor 3b, subunit 3 (Sf3b3) |
CNY 8,472.00 |
|
| MR211816L3 | Lenti ORF clone of Sf3b3 (Myc-DDK-tagged) - Mouse splicing factor 3b, subunit 3 (Sf3b3) |
CNY 11,500.00 |
|
| MR211816L4 | Lenti ORF clone of Sf3b3 (mGFP-tagged) - Mouse splicing factor 3b, subunit 3 (Sf3b3) |
CNY 11,500.00 |
