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Hmgcl (NM_008254) Mouse Tagged ORF Clone
CAT＃: MG204737
- TrueORF®
Hmgcl (tGFP-tagged) - Mouse 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl)
ORF Plasmid: DDK
"NM_008254" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW476067; HL |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204737 representing NM_008254
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCAGTGAGGAAGGCTTTCCCGCGGAGGCTGGTGGGCTTGACGTCCCTCCGGGCTGTGAGCACCT CCTCCATGGGCACTTTGCCAAAGCAGGTGAAGATCGTGGAAGTCGGTCCCCGAGATGGGCTGCAGAATGA AAAGAGTATTGTACCCACCCCAGTGAAGATCAGGCTGATCGACATGCTTTCCGAAGCAGGGCTCCCCGTG ATCGAGGCCACCAGCTTTGTTTCTCCCAAGTGGGTGCCACAGATGGCTGACCACTCTGACGTCTTGAAGG GCATTCAGAAGTTTCCCGGCATCAACTACCCAGTCCTGACTCCAAACATGAAAGGCTTTGAGGAAGCGGT AGCTGCGGGTGCCAAGGAAGTGAGCGTCTTCGGTGCTGTGTCTGAGCTCTTCACCCGGAAGAACGCGAAC TGCTCTATAGAGGAGAGTTTCCAGCGCTTTGCTGGAGTCATGCAGGCGGCCCAGGCCGCCAGCATCTCTG TGAGAGGGTATGTCTCCTGTGCCCTCGGATGCCCCTACGAGGGGAAGGTCTCCCCGGCTAAAGTTGCTGA GGTTGCCAAGAAGTTGTACTCGATGGGCTGCTATGAGATCTCCCTTGGGGACACCATCGGCGTAGGTACC CCAGGACTCATGAAAGACATGCTGACTGCTGTCATGCATGAAGTGCCTGTGACAGCATTGGCTGTCCACT GCCATGATACCTATGGTCAAGCTCTGGCCAACACCTTGGTGGCCCTGCAGATGGGTGTGAGTGTTGTGGA CTCCTCTGTGGCAGGACTTGGAGGCTGTCCCTATGCAAAAGGGGCATCAGGGAACTTGGCTACTGAGGAC CTGGTCTACATGCTGAATGGCTTAGGGATTCACACGGGTGTGAACCTCCAGAAACTTCTAGAAGCTGGGG ACTTCATCTGTCAAGCCCTTAACAGAAAAACTAGTTCCAAAGTGGCACAGGCCACCTGCAAGCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204737 representing NM_008254
Red=Cloning site Green=Tags(s) MASVRKAFPRRLVGLTSLRAVSTSSMGTLPKQVKIVEVGPRDGLQNEKSIVPTPVKIRLIDMLSEAGLPV IEATSFVSPKWVPQMADHSDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGAKEVSVFGAVSELFTRKNAN CSIEESFQRFAGVMQAAQAASISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGVGT PGLMKDMLTAVMHEVPVTALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPYAKGASGNLATED LVYMLNGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008254 |
| ORF Size | 975 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008254.3 |
| RefSeq Size | 1440 bp |
| RefSeq ORF | 978 bp |
| Locus ID | 15356 |
| UniProt ID | P38060 |
| Gene Summary | Mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis. Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202939 | Hmgcl (untagged) - Mouse 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl), (10ug) |
CNY 2,000.00 |
|
| MR204737 | Hmgcl (Myc-DDK-tagged) - Mouse 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl) |
CNY 2,081.00 |
|
| MR204737L3 | Lenti ORF clone of Hmgcl (Myc-DDK-tagged) - Mouse 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl) |
CNY 4,750.00 |
|
| MR204737L4 | Lenti ORF clone of Hmgcl (mGFP-tagged) - Mouse 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl) |
CNY 4,750.00 |
