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Epcam (NM_008532) Mouse Tagged ORF Clone
CAT＃: MG204481
- TrueORF®
Epcam (tGFP-tagged) - Mouse tumor-associated calcium signal transducer 1 (Tacstd1)
ORF Plasmid: DDK
"NM_008532" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CD326; EGP; EGP-2; Egp314; Ep-CAM; EpCAM1; GA733-2; gp40; Ly74; Tacsd1; Tacstd1; TROP1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204481 representing NM_008532
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGTCCCCAGGCCCTCGCGTTCGGGCTCCTGCTCGCGGTGGTCACAGCGACGCTGGCCGCGGCTC AGAGAGACTGTGTCTGTGACAACTACAAGCTGGCAACAAGTTGCTCTCTGAATGAATATGGTGAATGCCA GTGTACTTCCTATGGTACACAGAATACTGTCATTTGCTCCAAACTGGCGTCTAAATGCTTGGCGATGAAA GCAGAAATGACTCACAGCAAGTCTGGGAGGAGGATAAAGCCCGAAGGGGCGATCCAGAACAACGATGGGC TGTACGACCCCGACTGCGACGAGCAGGGGCTCTTCAAAGCCAAGCAGTGCAACGGCACCGCCACGTGCTG GTGTGTCAACACCGCCGGAGTCCGAAGAACCGACAAGGACACGGAGATCACGTGCTCCGAGCGCGTGAGG ACCTACTGGATCATCATTGAACTAAAACACAAAGAAAGAGAAAGCCCCTACGACCATCAGAGCTTGCAGA CTGCGCTTCAAGAGGCGTTCACATCTCGATATAAGCTGAATCAGAAATTTATCAAAAACATTATGTATGA GAATAATGTTATCACCATTGATCTGATGCAAAACTCTTCTCAGAAAACACAAGACGACGTGGACATAGCT GATGTGGCTTACTATTTTGAAAAAGATGTGAAGGGGGAGTCCCTGTTCCATTCTTCTAAGAGCATGGACC TGAGAGTGAACGGAGAGCCGCTCGATCTGGACCCCGGGCAGACTCTGATTTACTACGTTGATGAAAAGGC ACCCGAGTTCTCCATGCAGGGCCTCACGGCCGGGATCATCGCTGTCATTGTGGTGGTGTCATTAGCAGTC ATCGCGGGGATTGTTGTCCTGGTTATATCTACAAGGAAGAAATCAGCAAAATATGAGAAGGCTGAGATAA AGGAGATGGGTGAGATCCACAGAGAGCTTAATGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204481 representing NM_008532
Red=Cloning site Green=Tags(s) MAGPQALAFGLLLAVVTATLAAAQRDCVCDNYKLATSCSLNEYGECQCTSYGTQNTVICSKLASKCLAMK AEMTHSKSGRRIKPEGAIQNNDGLYDPDCDEQGLFKAKQCNGTATCWCVNTAGVRRTDKDTEITCSERVR TYWIIIELKHKERESPYDHQSLQTALQEAFTSRYKLNQKFIKNIMYENNVITIDLMQNSSQKTQDDVDIA DVAYYFEKDVKGESLFHSSKSMDLRVNGEPLDLDPGQTLIYYVDEKAPEFSMQGLTAGIIAVIVVVSLAV IAGIVVLVISTRKKSAKYEKAEIKEMGEIHRELNA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008532 |
| ORF Size | 945 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008532.2, NP_032558.2 |
| RefSeq Size | 2061 bp |
| RefSeq ORF | 948 bp |
| Locus ID | 17075 |
| UniProt ID | Q99JW5 |
| Gene Summary | May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203751 | Epcam (untagged) - Mouse epithelial cell adhesion molecule (Epcam), (10ug) |
CNY 2,000.00 |
|
| MR204481 | Epcam (Myc-DDK-tagged) - Mouse epithelial cell adhesion molecule (Epcam) |
CNY 2,081.00 |
|
| MR204481L3 | Lenti ORF clone of Epcam (Myc-DDK-tagged) - Mouse epithelial cell adhesion molecule (Epcam) |
CNY 4,750.00 |
|
| MR204481L4 | Lenti ORF clone of Epcam (mGFP-tagged) - Mouse epithelial cell adhesion molecule (Epcam) |
CNY 4,750.00 |
