United States
Germany
Japan
United Kingdom
China
Vapa (NM_013933) Mouse Tagged ORF Clone
CAT＃: MG203158
- TrueORF®
Vapa (tGFP-tagged) - Mouse vesicle-associated membrane protein, associated protein A (Vapa)
ORF Plasmid: DDK
"NM_013933" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 33kDa; VAP33 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203158 representing NM_013933
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCCGCCTCCGGGGCCATGGCGAAGCACGAGCAGATCCTGGTCCTCGACCCTCCTTCAGACCTCA AATTCAAAGGCCCCTTCACAGATGTAGTCACTACAAATCTTAAATTGCAAAATCCATCGGATAGAAAAGT GTGTTTCAAAGTGAAGACTACAGCACCTCGCCGGTACTGTGTGCGGCCCAACAGTGGGATTATTGACCCG GGGTCAATTGTGACTGTTTCAGTAATGCTGCAACCCTTTGATTATGATCCGAATGAAAAGAGTAAACATA AGTTCATGGTACAGACAATTTTTGCTCCACCAAACATTTCAGATATGGAAGCTGTGTGGAAAGAAGCAAA ACCTGATGAATTAATGGATTCTAAATTGAGATGTGTGTTTGAAATGCCGAATGAAAATGATAAGCTGAAT GATATGGAACCTAGCAAAGCTGTTCCACTGAATGCATCCAAACAAGACGGACCCCTGCCAAAACCACACA GTGTTTCACTCAATGATACGGAAACAAGGAAACTGATGGAAGAGTGCAAGCGACTCCAGGGAGAAATGAT GAAGCTCTCAGAAGAAAACCGACACCTGAGAGATGAAGGCCTAAGGCTCAGAAAGGTAGCACATTCGGAT AAACCTGGATCCACCTCAGCCGTGTCCTTCAGAGATAATGTCACCAGTCCTCTTCCTTCTCTTCTGGTTG TAATTGCAGCCATTTTCATTGGATTCTTTCTAGGGAAATTCATCTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203158 representing NM_013933
Red=Cloning site Green=Tags(s) MASASGAMAKHEQILVLDPPSDLKFKGPFTDVVTTNLKLQNPSDRKVCFKVKTTAPRRYCVRPNSGIIDP GSIVTVSVMLQPFDYDPNEKSKHKFMVQTIFAPPNISDMEAVWKEAKPDELMDSKLRCVFEMPNENDKLN DMEPSKAVPLNASKQDGPLPKPHSVSLNDTETRKLMEECKRLQGEMMKLSEENRHLRDEGLRLRKVAHSD KPGSTSAVSFRDNVTSPLPSLLVVIAAIFIGFFLGKFIL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013933 |
| ORF Size | 1655 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013933.3, NP_038961.2 |
| RefSeq Size | 1668 bp |
| RefSeq ORF | 750 bp |
| Locus ID | 30960 |
| UniProt ID | Q9WV55 |
| Gene Summary | Binds to OSBPL3, which mediates recruitment of VAPA to plasma membrane sites. The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface. With OSBPL3, may regulate ER morphology. May play a role in vesicle trafficking.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200063 | Vapa (untagged) - Mouse vesicle-associated membrane protein, associated protein A (Vapa), (10ug) |
CNY 2,000.00 |
|
| MR203158 | Vapa (Myc-DDK-tagged) - Mouse vesicle-associated membrane protein, associated protein A (Vapa) |
CNY 2,081.00 |
|
| MR203158L3 | Lenti ORF clone of Vapa (Myc-DDK-tagged) - Mouse vesicle-associated membrane protein, associated protein A (Vapa) |
CNY 4,750.00 |
|
| MR203158L4 | Lenti ORF clone of Vapa (mGFP-tagged) - Mouse vesicle-associated membrane protein, associated protein A (Vapa) |
CNY 4,750.00 |
