Rab22a (NM_024436) Mouse Tagged ORF Clone

CAT#: MG201880

  • TrueORF®

Rab22a (tGFP-tagged) - Mouse RAB22A, member RAS oncogene family (Rab22a)

ORF Plasmid: DDK tGFP



  "NM_024436" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 3732413A17Rik; AI662177; AW319644; AW550514; E130120E14Rik; Rab22
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG201880 representing NM_024436
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCTGAGGGAACTTAAAGTGTGCCTGCTCGGGGATACGGGTGTGGGTAAATCGAGCATCGTGTGGC
GGTTTGTGGAAGACAGTTTTGATCCGAATATCAATCCAACCATAGGGGCATCTTTTATGACCAAGACTGT
CCAGTACCAAAATGAGCTACATAAATTCCTAATCTGGGATACAGCTGGACAAGAACGATTTCGTGCATTG
GCACCAATGTACTATCGAGGGTCAGCAGCAGCCATCATCGTTTACGACATCACAAAAGAAGAGACGTTTT
CAACTCTGAAGAACTGGGTGAGAGAGCTCCGCCAGCACGGCCCCCCCAGCATTGTCGTTGCCATCGCAGG
GAACAAGTGCGATCTTACTGATGTCAGAGAAGTCATGGAGAGAGATGCTAAGGACTACGCAGATTCCATT
CATGCCATCTTTGTAGAGACCAGCGCAAAAAATGCAATAAACATAAATGAACTCTTTATAGAAATTAGTC
GAAGAATTCCATCCACTGATGCCAACCCAGCGTCTGGCGGCAAGGGCTTCAAACTCCGAAGACAGCCATC
GGAGCCAAAGCGAAGCTGCTGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG201880 representing NM_024436
Red=Cloning site Green=Tags(s)

MALRELKVCLLGDTGVGKSSIVWRFVEDSFDPNINPTIGASFMTKTVQYQNELHKFLIWDTAGQERFRAL
APMYYRGSAAAIIVYDITKEETFSTLKNWVRELRQHGPPSIVVAIAGNKCDLTDVREVMERDAKDYADSI
HAIFVETSAKNAININELFIEISRRIPSTDANPASGGKGFKLRRQPSEPKRSCC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_024436
ORF Size 582 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_024436.3
RefSeq Size 4385 bp
RefSeq ORF 585 bp
Locus ID 19334
UniProt ID P35285
Gene Summary Plays a role in endocytosis and intracellular protein transport (PubMed:19759177, PubMed:27718357). Mediates trafficking of TF from early endosomes to recycling endosomes. Required for NGF-mediated endocytosis of NTRK1, and subsequent neurite outgrowth. Binds GTP and GDP and has low GTPase activity. Alternates between a GTP-bound active form and a GDP-bound inactive form (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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