MDA5 (IFIH1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1 - 4 ug/mL, ICC: 5 ug/mL, IF: 20 ug/mL
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	MDA5 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human MDA5.
Formulation	PBS containing 0.02% sodium azide.
Concentration	1ug/ul
Purification	Affinity chromatography purified via peptide column
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	interferon induced with helicase C domain 1
Database Link	NP_071451 Entrez Gene 71586 MouseEntrez Gene 64135 Human UniProt ID Q9BYX4
Background	The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-kB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.
Synonyms	AGS7; Hlcd; IDDM19; MDA-5; MDA5; RLR-2
Reference Data
Protein Pathways	RIG-I-like receptor signaling pathway

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