CD3E Mouse Monoclonal Antibody [Clone ID: MEM-92]

Specifications

Product Data
Clone Name	MEM-92
Applications	FC, FN, IP
Recommend Dilution	Flow Cytometry: 2-10 μg/ml. Immunoprecipitation. Functional Application: The antibody MEM-92 in solution induces early responses of T cell activation (tyrosine phosphorylation, calcium elevation, Erk activation and expression of activation antigens), but it is unable to induce T cell proliferation.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human peripheral blood lymphocytes
Specificity	This antibody reacts with epsilon chain of human CD3 complex, a part of a bigger multisubunit complex of the T cell receptor (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.
Formulation	Azide Free PBS, pH~7.4, 0.2 μm filter sterilized State: Low Endotoxin State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: None
Concentration	lot specific
Purification	Thiophilic Adsorptio Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD3e molecule
Database Link	Entrez Gene 916 Human UniProt ID P07766
Background	CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T-cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Synonyms	T3/Leu-4
Reference Data

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