VAPA Mouse Monoclonal Antibody [Clone ID: OTI10E10]

Specifications

Product Data
Clone Name	OTI10E10
Applications	IHC, WB
Recommend Dilution	WB 1:2000, IHC 1:150
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant protein fragment corresponding to amino acids 2-227 of human VAPA (NP_919415) produced in E.coli.
Formulation	Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method	For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	27.7 kDa
Gene Name	VAMP associated protein A
Database Link	NP_919415 Entrez Gene 30960 MouseEntrez Gene 58857 RatEntrez Gene 9218 Human UniProt ID Q9P0L0
Background	The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
Synonyms	hVAP-33; VAMP-A; VAP-33; VAP-A; VAP33
Reference Data
Protein Families	Transmembrane
Protein Pathways	Tight junction

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