CD95 (FAS) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Sandwich ELISA: To detect Human sFas Receptor by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sFas Receptor (Cat.-No AP03248BT-N or AP03248BT-S) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human sFas Receptor.  Western Blot: To detect Human sFas Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human sFas Receptor is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly purified E.coli derived recombinant Human sFas Receptor (Cat.-No PA222)
Specificity	This antibody recognizes Human sFas Receptor.
Formulation	PBS, pH 7.2 State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml. Centrifuge vial prior to opening.
Purification	Affinity Chromatography employing an immobilized Human sFas Receptor matrix
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	Fas cell surface death receptor
Database Link	Entrez Gene 355 Human UniProt ID P25445
Synonyms	FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6
Reference Data

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