CD95 (FAS) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Human sFas Receptor by Direct ELISA (using 100 ml/well) a concentration of approximately 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2–0.4 ng/well of recombinant Human sFas Receptor.  Sandwich ELISA: To detect Human sFas Receptor by Sandwich ELISA (using 100 ml/well) a concentration of 0.25–1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human sFas Receptor (Cat.-No AP03248PU-N or AP03248PU-S) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of recombinant Human sFas Receptor.  Western Blot: To detect Human sFas Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Human sFas Receptor is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly purified E.coli derived recombinant Human sFas Receptor (Cat.-No PA222)
Specificity	This antibody recognizes Human sFas Receptor.
Formulation	PBS, pH 7.2 Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Centrifuge vial prior to opening.
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	Fas cell surface death receptor
Database Link	Entrez Gene 355 Human UniProt ID P25445
Synonyms	FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6
Reference Data

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